GCDH Antibody | Affinity Biosciences

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Western blot analysis of extracts from L02 cells, using GCDH Antibody.

Product:	GCDH Antibody
Catalog:	DF12614
Description:	Rabbit polyclonal antibody to GCDH
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	48 kDa; 48kD(Calculated).
Uniprot:	Q92947
RRID:	AB_2845576

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(83%), Dog(92%), Chicken(83%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

GCDH Antibody detects endogenous levels of total GCDH.

RRID:

AB_2845576
Cite Format: Affinity Biosciences Cat# DF12614, RRID:AB_2845576.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACAD5; EC 1.3.99.7; GCD; Gcdh; GCDH_HUMAN; Glutaryl CoA dehydrogenase; Glutaryl CoA dehydrogenase, mitochondrial; Glutaryl Coenzyme A dehydrogenase; Glutaryl-CoA dehydrogenase; mitochondrial; MS781;

Immunogens

Immunogen:

Uniprot:

Q92947(GCDH_HUMAN) >>Visit HPA database.

Gene(ID):

GCDH(2639)

Expression:

Q92947 GCDH_HUMAN:

Isoform Long and isoform Short are expressed in fibroblasts and liver.

Sequence:

Q92947 GCDH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALRGVSVRLLSRGPGLHVLRTWVSSAAQTEKGGRTQSQLAKSSRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFTASK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Dog

Chicken

Rabbit

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92947 As Substrate

Q92947

Site	PTM Type	Source
K32	Ubiquitination	Uniprot
R88	Methylation	Uniprot
K163	Ubiquitination	Uniprot
K202	Ubiquitination	Uniprot
K240	Ubiquitination	Uniprot
S246	Phosphorylation	Uniprot
S255	Phosphorylation	Uniprot
R319	Methylation	Uniprot
K361	Ubiquitination	Uniprot
K371	Ubiquitination	Uniprot
K377	Ubiquitination	Uniprot
Y398	Phosphorylation	Uniprot
K438	Acetylation	Uniprot

Research Backgrounds

Q92947_GCDH_HUMAN

Function:

Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.

Subcellular Location:

Mitochondrion matrix.

Tissue Specificity:

Isoform Long and isoform Short are expressed in fibroblasts and liver.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the acyl-CoA dehydrogenase family.

Research Fields

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Amino acid metabolism > Lysine degradation.

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GCDH Antibody - #DF12614