FLCN Antibody - #DF12608

(3)  
Product: FLCN Antibody
Catalog: DF12608
Description: Rabbit polyclonal antibody to FLCN
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 45 kDa,70kDa; 64kD(Calculated).
Uniprot: Q8NFG4
RRID: AB_2845570

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Rabbit(90%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FLCN Antibody detects endogenous levels of total FLCN.
RRID:
AB_2845570
Cite Format: Affinity Biosciences Cat# DF12608, RRID:AB_2845570.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BHD; BHD skin lesion fibrofolliculoma protein; Birt Hogg Dube syndrome protein; Birt-Hogg-Dube syndrome protein; DKFZp547A118; FLCL; Flcn; FLCN_HUMAN; FLJ45004; FLJ99377; Folliculin; MGC17998; MGC23445;

Immunogens

Immunogen:
Uniprot:

Q8NFG4(FLCN_HUMAN) >>Visit HPA database.

Gene(ID):
FLCN(201163)
Expression:
Q8NFG4 FLCN_HUMAN:

Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.

Sequence:
MNAIVALCHFCELHGPRTLFCTEVLHAPLPQGDGNEDSPGQGEQAEEEEGGIQMNSRMRAHSPAEGASVESSSPGPKKSDMCEGCRSLAAGHPGYISHDKETSIKYVSHQHPSHPQLFSIVRQACVRSLSCEVCPGREGPIFFGDEQHGFVFSHTFFIKDSLARGFQRWYSIITIMMDRIYLINSWPFLLGKVRGIIDELQGKALKVFEAEQFGCPQRAQRMNTAFTPFLHQRNGNAARSLTSLTSDDNLWACLHTSFAWLLKACGSRLTEKLLEGAPTEDTLVQMEKLADLEEESESWDNSEAEEEEKAPVLPESTEGRELTQGPAESSSLSGCGSWQPRKLPVFKSLRHMRQVLGAPSFRMLAWHVLMGNQVIWKSRDVDLVQSAFEVLRTMLPVGCVRIIPYSSQYEEAYRCNFLGLSPHVQIPPHVLSSEFAVIVEVHAAARSTLHPVGCEDDQSLSKYEFVVTSGSPVAADRVGPTILNKIEAALTNQNLSVDVVDQCLVCLKEEWMNKVKVLFKFTKVDSRPKEDTQKLLSILGASEEDNVKLLKFWMTGLSKTYKSHLMSTVRSPTASESRN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
90
Zebrafish
63
Horse
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NFG4 As Substrate

Site PTM Type Enzyme
S62 Phosphorylation P54646 (PRKAA2)
S68 Phosphorylation
S71 Phosphorylation
S72 Phosphorylation
S73 Phosphorylation
K100 Ubiquitination
K203 Ubiquitination
K206 Ubiquitination
T224 Phosphorylation
K272 Ubiquitination
S298 Phosphorylation
S302 Phosphorylation
S406 Phosphorylation
R477 Methylation
K534 Ubiquitination
S537 Phosphorylation
S542 Phosphorylation
S558 Phosphorylation
K559 Ubiquitination
K562 Ubiquitination
S571 Phosphorylation

Research Backgrounds

Function:

May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.

PTMs:

Phosphorylated. Several different phosphorylated forms exist.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Mainly localized in the nucleus. Colocalizes with FNIP1 and FNIP2 in the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.

Subunit Structure:

Interacts (via C-terminus) with FNIP1 and FNIP2 (via C-terminus). This mediates indirect interaction with the PRKAA1, PRKAB1 and PRKAG1 subunits of 5'-AMP-activated protein kinase. Interacts with HSP90AA1 in the presence of FNIP1. Interacts with HSP70, STUB1, CDC37, AHSA1, CCT2, STIP1, PTGES3 and PPP5C.

Family&Domains:

Belongs to the folliculin family.

Research Fields

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

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