DBNL Antibody - #DF12594

(2)  
Product: DBNL Antibody
Catalog: DF12594
Description: Rabbit polyclonal antibody to DBNL
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 55 kDa; 48kD(Calculated).
Uniprot: Q9UJU6
RRID: AB_2845556

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
DBNL Antibody detects endogenous levels of total DBNL.
RRID:
AB_2845556
Cite Format: Affinity Biosciences Cat# DF12594, RRID:AB_2845556.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABP1; Actin-binding protein 1; Cervical mucin associated protein; Cervical mucin-associated protein; Cervical SH3P7; CMAP; Dbnl; DBNL_HUMAN; Drebrin F; drebrin like; Drebrin like protein; Drebrin-F; Drebrin-like protein; HIP 55; HIP-55; HPK1 interacting protein of 55 kDa; HPK1-interacting protein of 55 kDa; SH3 domain containing protein 7; SH3 domain-containing protein 7; SH3P7; src homology 3 domain containing protein HIP 55;

Immunogens

Immunogen:
Uniprot:

Q9UJU6(DBNL_HUMAN) >>Visit HPA database.

Gene(ID):
DBNL(28988)
Sequence:
MAANLSRNGPALQEAYVRVVTEKSPTDWALFTYEGNSNDIRVAGTGEGGLEEMVEELNSGKVMYAFCRVKDPNSGLPKFVLINWTGEGVNDVRKGACASHVSTMASFLKGAHVTINARAEEDVEPECIMEKVAKASGANYSFHKESGRFQDVGPQAPVGSVYQKTNAVSEIKRVGKDSFWAKAEKEEENRRLEEKRRAEEAQRQLEQERRERELREAARREQRYQEQGGEASPQRTWEQQQEVVSRNRNEQESAVHPREIFKQKERAMSTTSISSPQPGKLRSPFLQKQLTQPETHFGREPAAAISRPRADLPAEEPAPSTPPCLVQAEEEAVYEEPPEQETFYEQPPLVQQQGAGSEHIDHHIQGQGLSGQGLCARALYDYQAADDTEISFDPENLITGIEVIDEGWWRGYGPDGHFGMFPANYVELIE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UJU6 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
Y16 Phosphorylation
T21 Phosphorylation
K23 Ubiquitination
S24 Phosphorylation
T26 Phosphorylation
T45 Phosphorylation
S99 Phosphorylation
S106 Phosphorylation
T114 Phosphorylation
C127 S-Nitrosylation
K131 Acetylation
K131 Ubiquitination
K134 Ubiquitination
S136 Phosphorylation
Y140 Phosphorylation
S141 Phosphorylation
K144 Methylation
K144 Ubiquitination
S146 Phosphorylation
S160 Phosphorylation
Y162 Phosphorylation
K164 Methylation
K164 Ubiquitination
T165 Phosphorylation
S169 Phosphorylation
K172 Methylation
K172 Ubiquitination
K176 Acetylation
K176 Ubiquitination
S178 Phosphorylation
Y224 Phosphorylation
S232 Phosphorylation
T236 Phosphorylation
S245 Phosphorylation
S269 Phosphorylation
T270 Phosphorylation
T271 Phosphorylation
S272 Phosphorylation
S274 Phosphorylation P24941 (CDK2)
S275 Phosphorylation P24941 (CDK2)
K280 Acetylation
K280 Ubiquitination
S283 Phosphorylation
K288 Acetylation
K288 Methylation
K288 Ubiquitination
T291 Phosphorylation
T295 Phosphorylation
S306 Phosphorylation
S320 Phosphorylation
T321 Phosphorylation
Y334 Phosphorylation P43403 (ZAP70)
Y344 Phosphorylation P43403 (ZAP70)

Research Backgrounds

Function:

Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes (By similarity). May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.

PTMs:

Degraded by caspases during apoptosis.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium. Cell projection>Ruffle. Cytoplasm>Cell cortex. Cytoplasm>Cytosol. Cell junction>Synapse. Perikaryon. Cell projection>Neuron projection. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>Clathrin-coated vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Podosome. Early endosome. Cell projection>Dendrite. Cell junction>Synapse>Postsynaptic density.
Note: Associates with lamellipodial actin and membrane ruffles. Colocalizes with actin and cortactin at podosome dots and podosome rosettes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SHANK2, SHANK3 and SYN1. Interacts with FGD1 and DNM1. Interacts with ANKRD54. Interacts with COBL. Interacts with WASL and WIPF1 (By similarity). Interacts with MAP4K1 and PRAM1.

Family&Domains:

The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.

Belongs to the ABP1 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.