Cytohesin2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells, using Cytohesin2 Antibody.

DF12592 at 1/100 staining Human ovarian cancer by IHC-P.

Product:	Cytohesin2 Antibody
Catalog:	DF12592
Description:	Rabbit polyclonal antibody to Cytohesin2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	47 kDa; 47kD(Calculated).
Uniprot:	Q99418
RRID:	AB_2845554

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Cytohesin2 Antibody detects endogenous levels of total Cytohesin2.

RRID:

AB_2845554
Cite Format: Affinity Biosciences Cat# DF12592, RRID:AB_2845554.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ARF exchange factor; ARF nucleotide binding site opener; ARF nucleotide-binding site opener; Arno; ARNO protein; CLM2; CTS18; CTS18.1; CYH2_HUMAN; Cyth2; Cytohesin 2; Cytohesin-2; MGC137537; MGC80440; PH; PH, SEC7 and coiled-coil domain-containing protein 2; Pleckstrin homology Sec7 and coiled coil domains 2; Pleckstrin homology Sec7 and coiled coil domains protein 2; Protein ARNO; PSCD2; PSCD2L; PSCD2L, formerly; SEC7 and coiled-coil domain-containing protein 2; Sec7; SEC7 homolog B; Sec7B; SEC7L; Sec7p L; Sec7p-like; Sec7pL;

Immunogens

Immunogen:

Uniprot:

Q99418(CYH2_HUMAN) >>Visit HPA database.

Gene(ID):

CYTH2(9266)

Expression:

Q99418 CYH2_HUMAN:

Widely expressed.

Sequence:

Q99418 CYH2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99418 As Substrate

Q99418

Site	PTM Type	Enzyme	Source
K26	Ubiquitination		Uniprot
S45	Phosphorylation		Uniprot
S56	Phosphorylation		Uniprot
K57	Ubiquitination		Uniprot
T58	Phosphorylation		Uniprot
K78	Ubiquitination		Uniprot
K102	Ubiquitination		Uniprot
K159	Ubiquitination		Uniprot
Y235	Phosphorylation		Uniprot
K244	Ubiquitination		Uniprot
K268	Ubiquitination		Uniprot
T276	Phosphorylation	P31749 (AKT1)	Uniprot
K319	Ubiquitination		Uniprot
T360	Phosphorylation		Uniprot
K364	Ubiquitination		Uniprot
Y381	Phosphorylation		Uniprot
S392	Phosphorylation	P05771 (PRKCB) , P05129 (PRKCG) , P17252 (PRKCA)	Uniprot

Research Backgrounds

Q99418_CYH2_HUMAN

Function:

Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Activates ARF factors through replacement of GDP with GTP (By similarity). The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Involved in neurite growth (By similarity).

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cytoplasm. Cell projection. Cell projection>Growth cone. Cell junction>Tight junction. Cell junction>Adherens junction.
Note: Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. They require also interaction with phosphoinositides for targeting to plasma membrane (PubMed:17398095). In differentiating neuroblastoma cells, colocalizes with CCDC120 in both neurite shaft and growth cone areas.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Heteromer. Composed of GRASP, CYTH2 and at least one GRM1 (By similarity). Interacts with ARRB1. Interacts with ARL4D; the interaction is direct. Directly interacts with CCDC120 through the coiled coil domain; this interaction stabilizes CCDC120, possibly by preventing its ubiquitination, and is required for neurite growth in neuroblastoma cells. Interacts with ARF1. Interacts with FRMD4A (By similarity). Interacts (via N-terminal domain) with INAVA (via N-terminal domain).

Family&Domains:

Binds via its PH domain to the inositol head group of phosphatidylinositol 1,4,5-trisphosphate. The PH domain is necessary and sufficient for plasma membrane relocalization.

Autoinhibited by its C-terminal basic region.

The coiled coil domain is involved in interaction with CCDC120.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

References

1). Candidate lncRNA-miRNA-mRNA network in predicting hepatocarcinogenesis with cirrhosis: an integrated bioinformatics analysis. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2020 (PubMed: 31758243) [IF=3.6]

Application: WB Species: human Sample: MHCC97H cells

Fig. 4| FOXD2-AS1/miR-139-5p/BLVRA or CYTH2 network contribute to the development of cirrhotic HCC. d Protein expressions of BLVRA, CYTH2, N-cadherin and E-cadherin in MHCC97H cells with altered levels of FOXD2-AS1 and miR-139-5p. e FOXD2-AS1,miR-139-5p, BLVRA and CYTH2 were signifcantly associated with the overall survival in patients with HCC, as detected using the Kaplan–Meier curve and a log-rank test. **P<0.01; *P<0.05

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For Research Use Only.
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Cytohesin2 Antibody - #DF12592