Product: Cytohesin2 Antibody
Catalog: DF12592
Description: Rabbit polyclonal antibody to Cytohesin2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 47 kDa; 47kD(Calculated).
Uniprot: Q99418
RRID: AB_2845554

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Cytohesin2 Antibody detects endogenous levels of total Cytohesin2.
RRID:
AB_2845554
Cite Format: Affinity Biosciences Cat# DF12592, RRID:AB_2845554.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARF exchange factor; ARF nucleotide binding site opener; ARF nucleotide-binding site opener; Arno; ARNO protein; CLM2; CTS18; CTS18.1; CYH2_HUMAN; Cyth2; Cytohesin 2; Cytohesin-2; MGC137537; MGC80440; PH; PH, SEC7 and coiled-coil domain-containing protein 2; Pleckstrin homology Sec7 and coiled coil domains 2; Pleckstrin homology Sec7 and coiled coil domains protein 2; Protein ARNO; PSCD2; PSCD2L; PSCD2L, formerly; SEC7 and coiled-coil domain-containing protein 2; Sec7; SEC7 homolog B; Sec7B; SEC7L; Sec7p L; Sec7p-like; Sec7pL;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99418 CYH2_HUMAN:

Widely expressed.

Sequence:
MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
75
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99418 As Substrate

Site PTM Type Enzyme
K26 Ubiquitination
S45 Phosphorylation
S56 Phosphorylation
K57 Ubiquitination
T58 Phosphorylation
K78 Ubiquitination
K102 Ubiquitination
K159 Ubiquitination
Y235 Phosphorylation
K244 Ubiquitination
K268 Ubiquitination
T276 Phosphorylation P31749 (AKT1)
K319 Ubiquitination
T360 Phosphorylation
K364 Ubiquitination
Y381 Phosphorylation
S392 Phosphorylation P05771 (PRKCB) , P05129 (PRKCG) , P17252 (PRKCA)

Research Backgrounds

Function:

Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Activates ARF factors through replacement of GDP with GTP (By similarity). The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Involved in neurite growth (By similarity).

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cytoplasm. Cell projection. Cell projection>Growth cone. Cell junction>Tight junction. Cell junction>Adherens junction.
Note: Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. They require also interaction with phosphoinositides for targeting to plasma membrane (PubMed:17398095). In differentiating neuroblastoma cells, colocalizes with CCDC120 in both neurite shaft and growth cone areas.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Heteromer. Composed of GRASP, CYTH2 and at least one GRM1 (By similarity). Interacts with ARRB1. Interacts with ARL4D; the interaction is direct. Directly interacts with CCDC120 through the coiled coil domain; this interaction stabilizes CCDC120, possibly by preventing its ubiquitination, and is required for neurite growth in neuroblastoma cells. Interacts with ARF1. Interacts with FRMD4A (By similarity). Interacts (via N-terminal domain) with INAVA (via N-terminal domain).

Family&Domains:

Binds via its PH domain to the inositol head group of phosphatidylinositol 1,4,5-trisphosphate. The PH domain is necessary and sufficient for plasma membrane relocalization.

Autoinhibited by its C-terminal basic region.

The coiled coil domain is involved in interaction with CCDC120.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

References

1). Candidate lncRNA-miRNA-mRNA network in predicting hepatocarcinogenesis with cirrhosis: an integrated bioinformatics analysis. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2020 (PubMed: 31758243) [IF=3.6]

Application: WB    Species: human    Sample: MHCC97H cells

Fig. 4| FOXD2-AS1/miR-139-5p/BLVRA or CYTH2 network contribute to the development of cirrhotic HCC. d Protein expressions of BLVRA, CYTH2, N-cadherin and E-cadherin in MHCC97H cells with altered levels of FOXD2-AS1 and miR-139-5p. e FOXD2-AS1,miR-139-5p, BLVRA and CYTH2 were signifcantly associated with the overall survival in patients with HCC, as detected using the Kaplan–Meier curve and a log-rank test. **P<0.01; *P<0.05

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