CORO1C Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from HeLa cells , using CORO1C Antibody.

Product:	CORO1C Antibody
Catalog:	DF12586
Description:	Rabbit polyclonal antibody to CORO1C
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	57 kDa; 53kD(Calculated).
Uniprot:	Q9ULV4
RRID:	AB_2845548

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

CORO1C Antibody detects endogenous levels of total CORO1C.

RRID:

AB_2845548
Cite Format: Affinity Biosciences Cat# DF12586, RRID:AB_2845548.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

COR1C_HUMAN; CORO 1C; Coro1c; Coronin 1c; Coronin actin binding protein 1C; Coronin-1C; Coronin-3; Coronin1c; Coronin3; CRNN 4; CRNN4; HCRNN 4; hCRNN4;

Immunogens

Immunogen:

Uniprot:

Q9ULV4(COR1C_HUMAN) >>Visit HPA database.

Gene(ID):

CORO1C(23603)

Expression:

Q9ULV4 COR1C_HUMAN:

Ubiquitous.

Sequence:

Q9ULV4 COR1C_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRRVVRQSKFRHVFGQAVKNDQCYDDIRVSRVTWDSSFCAVNPRFVAIIIEASGGGAFLVLPLHKTGRIDKSYPTVCGHTGPVLDIDWCPHNDQVIASGSEDCTVMVWQIPENGLTLSLTEPVVILEGHSKRVGIVAWHPTARNVLLSAGCDNAIIIWNVGTGEALINLDDMHSDMIYNVSWNRNGSLICTASKDKKVRVIDPRKQEIVAEKEKAHEGARPMRAIFLADGNVFTTGFSRMSERQLALWNPKNMQEPIALHEMDTSNGVLLPFYDPDTSIIYLCGKGDSSIRYFEITDESPYVHYLNTFSSKEPQRGMGYMPKRGLDVNKCEIARFFKLHERKCEPIIMTVPRKSDLFQDDLYPDTAGPEAALEAEEWFEGKNADPILISLKHGYIPGKNRDLKVVKKNILDSKPTANKKCDLISIPKKTTDTASVQNEAKLDEILKEIKSIKDTICNQDERISKLEQQMAKIAA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULV4 As Substrate

Q9ULV4

Site	PTM Type	Enzyme	Source
K19	Acetylation		Uniprot
R28	Methylation		Uniprot
S187	Phosphorylation		Uniprot
R291	Methylation		Uniprot
Y292	Phosphorylation		Uniprot
S299	Phosphorylation		Uniprot
Y301	Phosphorylation		Uniprot
Y304	Phosphorylation		Uniprot
K311	Ubiquitination		Uniprot
K322	Ubiquitination		Uniprot
K329	Ubiquitination		Uniprot
K337	Acetylation		Uniprot
K353	Ubiquitination		Uniprot
S389	Phosphorylation		Uniprot
K391	Ubiquitination		Uniprot
Y394	Phosphorylation		Uniprot
K398	Ubiquitination		Uniprot
K407	Ubiquitination		Uniprot
K413	Acetylation		Uniprot
K413	Ubiquitination		Uniprot
T415	Phosphorylation		Uniprot
K418	Acetylation		Uniprot
S424	Phosphorylation		Uniprot
T430	Phosphorylation		Uniprot
S434	Phosphorylation		Uniprot
K440	Acetylation		Uniprot
K446	Acetylation		Uniprot
K446	Ubiquitination		Uniprot
S463	Phosphorylation	P68400 (CSNK2A1)	Uniprot
K464	Ubiquitination		Uniprot
K471	Ubiquitination		Uniprot

Research Backgrounds

Q9ULV4_COR1C_HUMAN

Function:

Plays a role in directed cell migration by regulating the activation and subcellular location of RAC1. Increases the presence of activated RAC1 at the leading edge of migrating cells. Required for normal organization of the cytoskeleton, including the actin cytoskeleton, microtubules and the vimentin intermediate filaments (By similarity). Plays a role in endoplasmic reticulum-associated endosome fission: localizes to endosome membrane tubules and promotes recruitment of TMCC1, leading to recruitment of the endoplasmic reticulum to endosome tubules for fission. Endosome membrane fission of early and late endosomes is essential to separate regions destined for lysosomal degradation from carriers to be recycled to the plasma membrane. Required for normal cell proliferation, cell migration, and normal formation of lamellipodia (By similarity). Required for normal distribution of mitochondria within cells (By similarity).

Involved in myogenic differentiation.

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium. Cell projection>Ruffle membrane. Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Endosome membrane.
Note: All isoforms colocalize with the actin cytoskeleton in the cytosol, and especially in the cell cortex (PubMed:10828594, PubMed:19651142, PubMed:25074804). Colocalizes with F-actin at the leading edge of lamellipodia. Partially colocalizes with microtubules and vimentin intermediate filaments (PubMed:10828594, PubMed:19651142, PubMed:25074804). Localizes to endosome membrane tubules/buds (PubMed:30220460).

Cell membrane>Sarcolemma. Cytoplasm>Myofibril>Sarcomere. Cell junction>Synapse. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex.
Note: Colocalizes with the thin filaments of the sarcomere and with the postsynaptic area and the junctional sarcoplasm of motor end plates. Colocalizes with the actin cytoskeleton in the cytosol, and especially in the cell cortex.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Binds F-actin. Interacts with RCC2. Interacts preferentially with nucleotide-free and GDP-bound RAC1. Interacts with VIM (via head domain) (By similarity). Isoform 1 and isoform 2 appear as homotrimers, while isoform 3 seems to exist as monomers.

Family&Domains:

The C-terminal coiled-coil domain is essential for cortical membrane localization and oligomerization.

Belongs to the WD repeat coronin family.

References

1). Circ_0025039 acts an oncogenic role in the progression of non-small cell lung cancer through miR-636-dependent regulation of CORO1C. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2022 (PubMed: 35034254) [IF=4.3]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CORO1C Antibody - #DF12586