COQ6 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse heart tissue, using COQ6 Antibody.

Product:	COQ6 Antibody
Catalog:	DF12585
Description:	Rabbit polyclonal antibody to COQ6
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	51 kDa; 51kD(Calculated).
Uniprot:	Q9Y2Z9
RRID:	AB_2845547

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(83%)

Clonality:

Polyclonal

Specificity:

COQ6 Antibody detects endogenous levels of total COQ6.

RRID:

AB_2845547
Cite Format: Affinity Biosciences Cat# DF12585, RRID:AB_2845547.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CGI-10; Coenzyme Q6 homolog (yeast); Coenzyme Q6 homolog, monooxygenase (S. cerevisiae); Coenzyme Q6 homolog, monooxygenase (yeast); coq6; COQ6_HUMAN; Ubiquinone biosynthesis monooxygenase COQ6;

Immunogens

Immunogen:

Uniprot:

Q9Y2Z9(COQ6_HUMAN) >>Visit HPA database.

Gene(ID):

COQ6(51004)

Expression:

Q9Y2Z9 COQ6_HUMAN:

Widely expressed.

Sequence:

Q9Y2Z9 COQ6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAARLVSRCGAVRAAPHSGPLVSWRRWSGASTDTVYDVVVSGGGLVGAAMACALGYDIHFHDKKILLLEAGPKKVLEKLSETYSNRVSSISPGSATLLSSFGAWDHICNMRYRAFRRMQVWDACSEALIMFDKDNLDDMGYIVENDVIMHALTKQLEAVSDRVTVLYRSKAIRYTWPCPFPMADSSPWVHITLGDGSTFQTKLLIGADGHNSGVRQAVGIQNVSWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMDEEKFVDAVNSAFWSDADHTDFIDTAGAMLQYAVSLLKPTKVSARQLPPSVARVDAKSRVLFPLGLGHAAEYVRPRVALIGDAAHRVHPLAGQGVNMGFGDISSLAHHLSTAAFNGKDLGSVSHLTGYETERQRHNTALLAATDLLKRLYSTSASPLVLLRTWGLQATNAVSPLKEQIMAFASK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

Bovine

Sheep

Pig

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2Z9 As Substrate

Q9Y2Z9

Site	PTM Type	Source
K74	Acetylation	Uniprot
K78	Ubiquitination	Uniprot
S88	Phosphorylation	Uniprot
S327	Phosphorylation	Uniprot
S334	Phosphorylation	Uniprot
T414	Phosphorylation	Uniprot
K459	Ubiquitination	Uniprot

Research Backgrounds

Q9Y2Z9_COQ6_HUMAN

Function:

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-hexaprenyl-4-hydroxybenzoic acid (HHB) to 3-hexaprenyl-4,5-dihydroxybenzoic acid (DHHB). The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin/ferredoxin reductase system to COQ6 (By similarity). Is able to perform the deamination reaction at C4 of 3-hexaprenyl-4-amino-5-hydroxybenzoic acid (HHAB) to produce DHHB when expressed in yeast cells lacking COQ9, even if utilization of para-aminobenzoic acid (pABA) involving C4-deamination seems not to occur in bacteria, plants and mammals, where only C5 hydroxylation of HHB has been shown.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side. Golgi apparatus. Cell projection.
Note: Localizes to cell processes and Golgi apparatus in podocytes.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9 (By similarity). Interacts with COQ8B and COQ7.

Family&Domains:

Belongs to the UbiH/COQ6 family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Ubiquinone and other terpenoid-quinone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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COQ6 Antibody - #DF12585