CNOT3 Antibody - #DF12583
| Product: | CNOT3 Antibody |
| Catalog: | DF12583 |
| Description: | Rabbit polyclonal antibody to CNOT3 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 120 kDa; 82kD(Calculated). |
| Uniprot: | O75175 |
| RRID: | AB_2845545 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12583, RRID:AB_2845545.
Fold/Unfold
CCR4 associated factor 3; CCR4-associated factor 3; CCR4-NOT transcription complex subunit 3; CNOT3; CNOT3_HUMAN; KIAA0691; LENG2; Leukocyte receptor cluster member 2; negative regulator of transcription 3, S. cerevisiae, homolog of; NOT3 (negative regulator of transcription 3, yeast) homolog; NOT3; NOT3H;
Immunogens
Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
- O75175 CNOT3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKKEIKKLQRLRDQIKTWVASNEIKDKRQLIDNRKLIETQMERFKVVERETKTKAYSKEGLGLAQKVDPAQKEKEEVGQWLTNTIDTLNMQVDQFESEVESLSVQTRKKKGDKDKQDRIEGLKRHIEKHRYHVRMLETILRMLDNDSILVDAIRKIKDDVEYYVDSSQDPDFEENEFLYDDLDLEDIPQALVATSPPSHSHMEDEIFNQSSSTPTSTTSSSPIPPSPANCTTENSEDDKKRGRSTDSEVSQSPAKNGSKPVHSNQHPQSPAVPPTYPSGPPPAASALSTTPGNNGVPAPAAPPSALGPKASPAPSHNSGTPAPYAQAVAPPAPSGPSTTQPRPPSVQPSGGGGGGSGGGGSSSSSNSSAGGGAGKQNGATSYSSVVADSPAEVALSSSGGNNASSQALGPPSGPHNPPPSTSKEPSAAAPTGAGGVAPGSGNNSGGPSLLVPLPVNPPSSPTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPVPTLHLTERDIILSSTSAPPASAQPPLQLSEVNIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHHMPHPSDSERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75175 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K41 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| T100 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| T292 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S506 | Phosphorylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| S541 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| R635 | Methylation | Uniprot | |
| T645 | Phosphorylation | Uniprot | |
| Y685 | Phosphorylation | Uniprot | |
| K689 | Ubiquitination | Uniprot | |
| K737 | Ubiquitination | Uniprot |
Research Backgrounds
Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.
Cytoplasm. Nucleus. Cytoplasm>P-body.
Note: NANOS2 promotes its localization to P-body.
Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2.
Belongs to the CNOT2/3/5 family.
Research Fields
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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