BNIP1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HeLa cells, using BNIP1 Antibody.

Product:	BNIP1 Antibody
Catalog:	DF12569
Description:	Rabbit polyclonal antibody to BNIP1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	26 kDa; 26kD(Calculated).
Uniprot:	Q12981
RRID:	AB_2845531

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(83%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

BNIP1 Antibody detects endogenous levels of total BNIP1.

RRID:

AB_2845531
Cite Format: Affinity Biosciences Cat# DF12569, RRID:AB_2845531.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BCL2 adenovirus E1B 19kD interacting protein 1; BCL2/adenovirus E1B 19 kDa protein-interacting protein 1; BNIP1; MGC41600; Nip1; OTTHUMP00000161077; OTTHUMP00000161078; OTTHUMP00000161079; OTTHUMP00000223580; sec20; SEC20_HUMAN; SEC20L; Transformation-related gene 8 protein; TRG-8; TRG8; Vesicle transport protein SEC20;

Immunogens

Immunogen:

Uniprot:

Q12981(SEC20_HUMAN) >>Visit HPA database.

Gene(ID):

BNIP1(662)

Expression:

Q12981 SEC20_HUMAN:

Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine.

Sequence:

Q12981 SEC20_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPQDVHVRICNQEIVKFDLEVKALIQDIRDCSGPLSALTELNTKVKEKFQQLRHRIQDLEQLAKEQDKESEKQLLLQEVENHKKQMLSNQASWRKANLTCKIAIDNLEKAELLQGGDLLRQRKTTKESLAQTSSTITESLMGISRMMAQQVQQSEEAMQSLVTSSRTILDANEEFKSMSGTIQLGRKLITKYNRRELTDKLLIFLALALFLATVLYIVKKRLFPFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Chicken

100

Pig

Horse

Sheep

Dog

Rabbit

Bovine

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12981 As Substrate

Q12981

Site	PTM Type	Source
K46	Acetylation	Uniprot
K46	Ubiquitination	Uniprot
K48	Acetylation	Uniprot
K48	Methylation	Uniprot
K50	Methylation	Uniprot
K50	Ubiquitination	Uniprot
K66	Ubiquitination	Uniprot
K74	Ubiquitination	Uniprot
K86	Ubiquitination	Uniprot
S90	Phosphorylation	Uniprot
S94	Phosphorylation	Uniprot
K103	Ubiquitination	Uniprot
K111	Ubiquitination	Uniprot
T126	Phosphorylation	Uniprot
T127	Phosphorylation	Uniprot
K128	Ubiquitination	Uniprot
K178	Ubiquitination	Uniprot
S181	Phosphorylation	Uniprot
K189	Acetylation	Uniprot
K193	Acetylation	Uniprot
T200	Phosphorylation	Uniprot
T215	Phosphorylation	Uniprot
Y218	Phosphorylation	Uniprot

Research Backgrounds

Q12981_SEC20_HUMAN

Function:

As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Plays also a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable).

PTMs:

Polyubiquitinated. 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1. Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type IV membrane protein. Mitochondrion membrane>Single-pass type IV membrane protein.
Note: Localization to the mitochondrion is regulated by RNF186.

Tissue Specificity:

Subunit Structure:

Component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B. Interacts directly with STX18, RINT1/TIP20L and NAPA. Interacts with ZW10 through RINT1. Interacts with BCL2. Interacts with RNF186. Interacts with RNF185. Interacts with SQSTM1; increased by 'Lys-63'-linked polyubiquitination of BNIP1.

(Microbial infection) Interacts with adenovirus E1B 19K protein; plays a role in the suppression of cell apoptosis by the viral protein.

Family&Domains:

Belongs to the SEC20 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

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BNIP1 Antibody - #DF12569