ANGPTL2 Antibody - #DF12557

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Product: ANGPTL2 Antibody
Catalog: DF12557
Description: Rabbit polyclonal antibody to ANGPTL2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 57 kDa; 57kD(Calculated).
Uniprot: Q9UKU9
RRID: AB_2845519

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
ANGPTL2 Antibody detects endogenous levels of total ANGPTL2.
RRID:
AB_2845519
Cite Format: Affinity Biosciences Cat# DF12557, RRID:AB_2845519.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI593246; Angiopoietin like 2; Angiopoietin related protein 2; Angiopoietin-like protein 2; Angiopoietin-related protein 2; ANGL2_HUMAN; Angptl2; Arp2; AW260363; HARP; MGC8889; UNQ170/PRO196;

Immunogens

Immunogen:
Uniprot:

Q9UKU9(ANGL2_HUMAN) >>Visit HPA database.

Gene(ID):
ANGPTL2(23452)
Expression:
Q9UKU9 ANGL2_HUMAN:

Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.

Sequence:
MRPLCVTCWWLGLLAAMGAVAGQEDGFEGTEEGSPREFIYLNRYKRAGESQDKCTYTFIVPQQRVTGAICVNSKEPEVLLENRVHKQELELLNNELLKQKRQIETLQQLVEVDGGIVSEVKLLRKESRNMNSRVTQLYMQLLHEIIRKRDNALELSQLENRILNQTADMLQLASKYKDLEHKYQHLATLAHNQSEIIAQLEEHCQRVPSARPVPQPPPAAPPRVYQPPTYNRIINQISTNEIQSDQNLKVLPPPLPTMPTLTSLPSSTDKPSGPWRDCLQALEDGHDTSSIYLVKPENTNRLMQVWCDQRHDPGGWTVIQRRLDGSVNFFRNWETYKQGFGNIDGEYWLGLENIYWLTNQGNYKLLVTMEDWSGRKVFAEYASFRLEPESEYYKLRLGRYHGNAGDSFTWHNGKQFTTLDRDHDVYTGNCAHYQKGGWWYNACAHSNLNGVWYRGGHYRSRYQDGVYWAEFRGGSYSLKKVVMMIRPNPNTFH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
89
Chicken
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKU9 As Substrate

Site PTM Type Enzyme
S73 Phosphorylation
K86 Methylation
T229 O-Glycosylation
Y426 Phosphorylation
Y433 Phosphorylation
Y453 Phosphorylation
Y462 Phosphorylation
Y467 Phosphorylation
Y476 Phosphorylation

Research Backgrounds

Function:

Induces sprouting in endothelial cells through an autocrine and paracrine action.

PTMs:

N-glycosylated.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.

References

1). Anti-inflammatory activity of berberine in non-alcoholic fatty liver disease via the Angptl2 pathway. BMC IMMUNOLOGY, 2020 (PubMed: 32429849) [IF=3.0]

Application: WB    Species: rat    Sample: liver

Fig. 5 |The protein levels of Angptl2, Foxo1, NF-κB, p-NF-κB and CCR2 were measured by Western blot analysis and normalized by GAPDH expression. Data are presented as mean ± SD from eight rats. **P < 0.01,* P < 0.05.n = 8/group

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