AMOTL1 Antibody - #DF12556
(1)
| Product: | AMOTL1 Antibody |
| Catalog: | DF12556 |
| Description: | Rabbit polyclonal antibody to AMOTL1 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Rabbit, Dog |
| Mol.Wt.: | 107 kDa; 107kD(Calculated). |
| Uniprot: | Q8IY63 |
| RRID: | AB_2845518 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Horse(91%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
AMOTL1 Antibody detects endogenous levels of total AMOTL1.
RRID:
AB_2845518
Cite Format: Affinity Biosciences Cat# DF12556, RRID:AB_2845518.
Cite Format: Affinity Biosciences Cat# DF12556, RRID:AB_2845518.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
2310010G08Rik; 2310067L22Rik; 4932416D09Rik; AMOL1_HUMAN; Amotl1; Angiomotin-like protein 1; FLJ00155; JEAP; Junction enriched and associated protein; mFLJ00155;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q8IY63 AMOL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MWRAKLRRGTCEPAVKGSPSACYSPSSPVQVLEDSTYFSPDFQLYSGRHETSALTVEATSSIREKVVEDPLCNFHSPNFLRISEVEMRGSEDAAAGTVLQRLIQEQLRYGTPTENMNLLAIQHQATGSAGPAHPTNNFSSTENLTQEDPQMVYQSARQEPQGQEHQVDNTVMEKQVRSTQPQQNNEELPTYEEAKAQSQFFRGQQQQQQQQGAVGHGYYMAGGTSQKSRTEGRPTVNRANSGQAHKDEALKELKQGHVRSLSERIMQLSLERNGAKQHLPGSGNGKGFKVGGGPSPAQPAGKVLDPRGPPPEYPFKTKQMMSPVSKTQEHGLFYGDQHPGMLHEMVKPYPAPQPVRTDVAVLRYQPPPEYGVTSRPCQLPFPSTMQQHSPMSSQTSSASGPLHSVSLPLPLPMALGAPQPPPAASPSQQLGPDAFAIVERAQQMVEILTEENRVLHQELQGYYDNADKLHKFEKELQRISEAYESLVKSTTKRESLDKAMRNKLEGEIRRLHDFNRDLRDRLETANRQLSSREYEGHEDKAAEGHYASQNKEFLKEKEKLEMELAAVRTASEDHRRHIEILDQALSNAQARVIKLEEELREKQAYVEKVEKLQQALTQLQSACEKREQMERRLRTWLERELDALRTQQKHGNGQPANMPEYNAPALLELVREKEERILALEADMTKWEQKYLEESTIRHFAMNAAATAAAERDTTIINHSRNGSYGESSLEAHIWQEEEEVVQANRRCQDMEYTIKNLHAKIIEKDAMIKVLQQRSRKDAGKTDSSSLRPARSVPSIAAATGTHSRQTSLTSSQLAEEKKEEKTWKGSIGLLLGKEHHEHASAPLLPPPPTSALSSIASTTAASSAHAKTGSKDSSTQTDKSAELFWPSMASLPSRGRLSTTPAHSPVLKHPAAKGTAEKLENSPGHGKSPDHRGRVSSLLHKPEFPDGEMMEVLI
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
100
Dog
100
Horse
91
Pig
82
Bovine
73
Sheep
73
Chicken
73
Xenopus
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IY63 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K16 | Ubiquitination | Uniprot | |
| T51 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| Y191 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| Y218 | Phosphorylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K316 | Methylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K318 | Methylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| T373 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| Y546 | Phosphorylation | Uniprot | |
| K551 | Ubiquitination | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| Y661 | Phosphorylation | Uniprot | |
| K686 | Ubiquitination | Uniprot | |
| K690 | Ubiquitination | Uniprot | |
| T707 | Phosphorylation | Uniprot | |
| T714 | Phosphorylation | Uniprot | |
| T715 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S724 | Phosphorylation | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| K761 | Ubiquitination | Uniprot | |
| K765 | Ubiquitination | Uniprot | |
| K782 | Ubiquitination | Uniprot | |
| S793 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S796 | Phosphorylation | Uniprot | |
| T803 | Phosphorylation | Uniprot | |
| S805 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S813 | Phosphorylation | Uniprot | |
| S828 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| T877 | Phosphorylation | Uniprot | |
| T879 | Phosphorylation | Uniprot | |
| S892 | Phosphorylation | Uniprot | |
| S895 | Phosphorylation | Uniprot | |
| S900 | Phosphorylation | Uniprot | |
| T901 | Phosphorylation | Uniprot | |
| T902 | Phosphorylation | Uniprot | |
| S906 | Phosphorylation | Uniprot | |
| T917 | Phosphorylation | Uniprot | |
| S924 | Phosphorylation | Uniprot | |
| S930 | Phosphorylation | Uniprot | |
| S939 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus.
PTMs:
Polyubiquitinated by NEDD4, leading to proteasomal degradation.
Subcellular Location:
Cell junction>Tight junction.
Family&Domains:
Belongs to the angiomotin family.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
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