SIKE1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HEK-293 cells, using SIKE1 Antibody.

Product:	SIKE1 Antibody
Catalog:	DF12548
Description:	Rabbit polyclonal antibody to SIKE1
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	24 kDa; 24kD(Calculated).
Uniprot:	Q9BRV8
RRID:	AB_2845510

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

SIKE1 Antibody detects endogenous levels of total SIKE1.

RRID:

AB_2845510
Cite Format: Affinity Biosciences Cat# DF12548, RRID:AB_2845510.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ21168; RGD1311316; RP5-1000E10.4; SIKE; sike1; SIKE1_HUMAN; Suppressor of IKBKE 1; Suppressor of IKK-epsilon;

Immunogens

Immunogen:

Uniprot:

Q9BRV8(SIKE1_HUMAN) >>Visit HPA database.

Gene(ID):

SIKE1(80143)

Expression:

Q9BRV8 SIKE1_HUMAN:

Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level).

Sequence:

Q9BRV8 SIKE1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSCTIEKILTDAKTLLERLREHDAAAESLVDQSAALHRRVAAMREAGTALPDQYQEDASDMKDMSKYKPHILLSQENTQIRDLQQENRELWISLEEHQDALELIMSKYRKQMLQLMVAKKAVDAEPVLKAHQSHSAEIESQIDRICEMGEVMRKAVQVDDDQFCKIQEKLAQLELENKELRELLSISSESLQARKENSMDTASQAIK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BRV8 As Substrate

Q9BRV8

Site	PTM Type	Enzyme	Source
K13	Ubiquitination		Uniprot
K62	Ubiquitination		Uniprot
K68	Ubiquitination		Uniprot
S74	Phosphorylation		Uniprot
K120	Ubiquitination		Uniprot
K129	Ubiquitination		Uniprot
S133	Phosphorylation	Q9UHD2 (TBK1)	Uniprot
K154	Ubiquitination		Uniprot
K165	Ubiquitination		Uniprot
K169	Ubiquitination		Uniprot
K178	Ubiquitination		Uniprot
S185	Phosphorylation	Q9UHD2 (TBK1)	Uniprot
S187	Phosphorylation	Q9UHD2 (TBK1)	Uniprot
S188	Phosphorylation	Q9UHD2 (TBK1)	Uniprot
S190	Phosphorylation	Q9UHD2 (TBK1)	Uniprot
K195	Ubiquitination		Uniprot
S198	Phosphorylation	Q9UHD2 (TBK1)	Uniprot

Research Backgrounds

Q9BRV8_SIKE1_HUMAN

Function:

Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Subunit Structure:

Interacts with IKBKE and TBK1 via its coiled coil region. Interaction with TBK1 is disrupted upon viral infection or TLR3 stimulation. Interacts with CDC42BPB.

Family&Domains:

Belongs to the SIKE family.

Research Fields

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)

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SIKE1 Antibody - #DF12548