Phospho-SHP2 (Tyr580)[Tyr584] Antibody

IHC
pElisa

1/12

AF3413 at 1/200 staining human kidney tissue sections by IHC-P.

AF3413 at 1/100 staining Human gastric cancer by IHC-P.

AF3413 at 1/100 staining Human normal tissues adjacent to colorectal cancer by IHC-P.

AF3413 at 1/100 staining Human colorectal cancer by IHC-P.

AF3413 at 1/100 staining Human normal tissues adjacent to pancreatic cancer by IHC-P.

AF3413 at 1/100 staining Human pancreatic cancer by IHC-P.

AF3413 at 1/100 staining Human normal tissues adjacent to liver cancer by IHC-P.

AF3413 at 1/100 staining Human liver cancer by IHC-P.

peptide-ELISA analysis of AF3413.

Product:	Phospho-SHP2 (Tyr580)[Tyr584] Antibody
Catalog:	AF3413
Description:	Rabbit polyclonal antibody to Phospho-SHP2 (Tyr580)[Tyr584]
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	72kDa; 68kD(Calculated).
Uniprot:	Q06124
RRID:	AB_2834840

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Phospho-SHP2 (Tyr580) Antibody detects endogenous levels of SHP2 only when phosphorylated at Tyr584, which site historically referenced as Tyr580.

RRID:

AB_2834840
Cite Format: Affinity Biosciences Cat# AF3413, RRID:AB_2834840.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BPTP3; CFC; JMML; METCDS; MGC14433; NS1; OTTHUMP00000166107; OTTHUMP00000166108; Protein tyrosine phosphatase 2; Protein tyrosine phosphatase 2C; Protein tyrosine phosphatase non receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTN11_HUMAN; PTP-1D; PTP-2C; PTP1D; PTP2C; PTPN11; SAP2; SH-PTP2; SH-PTP3; SH2 domain containing protein tyrosine phosphatase 2; SHP 2; SHP-2; Shp2; SHPTP2; SHPTP3; Syp; Tyrosine-protein phosphatase non-receptor type 11;

Immunogens

Immunogen:

Uniprot:

Q06124(PTN11_HUMAN) >>Visit HPA database.

Gene(ID):

PTPN11(5781)

Expression:

Q06124 PTN11_HUMAN:

Widely expressed, with highest levels in heart, brain, and skeletal muscle.

Description:

SHP-2 a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death.

Sequence:

Q06124 PTN11_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Chicken

100

Pig

Bovine

Sheep

Dog

Rabbit

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q06124 As Substrate

Q06124

Site	PTM Type	Enzyme	Source
T2	Acetylation		Uniprot
S28	Phosphorylation		Uniprot
S34	Phosphorylation		Uniprot
S36	Phosphorylation		Uniprot
T59	Phosphorylation		Uniprot
Y62	Phosphorylation		Uniprot
Y63	Phosphorylation		Uniprot
Y66	Phosphorylation		Uniprot
T73	Phosphorylation		Uniprot
Y80	Phosphorylation		Uniprot
K120	Ubiquitination		Uniprot
K124	Ubiquitination		Uniprot
K131	Ubiquitination		Uniprot
S142	Phosphorylation		Uniprot
T153	Phosphorylation		Uniprot
S165	Phosphorylation		Uniprot
K178	Acetylation		Uniprot
Y179	Phosphorylation		Uniprot
S189	Phosphorylation		Uniprot
K198	Acetylation		Uniprot
K198	Ubiquitination		Uniprot
K235	Ubiquitination		Uniprot
K242	Ubiquitination		Uniprot
K244	Ubiquitination		Uniprot
K260	Ubiquitination		Uniprot
Y263	Phosphorylation		Uniprot
S264	Phosphorylation		Uniprot
K274	Acetylation		Uniprot
K276	Acetylation		Uniprot
Y279	Phosphorylation		Uniprot
K280	Acetylation		Uniprot
K280	Ubiquitination		Uniprot
S302	Phosphorylation		Uniprot
Y304	Phosphorylation		Uniprot
K317	Ubiquitination		Uniprot
S326	Phosphorylation		Uniprot
Y327	Phosphorylation		Uniprot
K389	Ubiquitination		Uniprot
T415	Phosphorylation		Uniprot
K486	Acetylation		Uniprot
Y515	Phosphorylation		Uniprot
Y519	Phosphorylation		Uniprot
S539	Phosphorylation		Uniprot
K542	Ubiquitination		Uniprot
Y546	Phosphorylation	P08922 (ROS1) , P09619 (PDGFRB) , P06241 (FYN) , Q9UM73 (ALK)	Uniprot
T547	Phosphorylation		Uniprot
Y551	Phosphorylation		Uniprot
S552	Phosphorylation		Uniprot
T557	Phosphorylation		Uniprot
S558	Phosphorylation		Uniprot
S562	Phosphorylation		Uniprot
T568	Phosphorylation		Uniprot
T570	Phosphorylation		Uniprot
S580	Phosphorylation	P17252 (PRKCA) , P05771 (PRKCB) , P24723 (PRKCH)	Uniprot
Y584	Phosphorylation	P08922 (ROS1) , P00519 (ABL1) , Q9UM73 (ALK) , P42684 (ABL2)	Uniprot
K594	Ubiquitination		Uniprot
S595	Phosphorylation	P05771-2 (PRKCB) , P24723 (PRKCH) , P17252 (PRKCA)	Uniprot

PTMs - Q06124 As Enzyme

Q06124

Substrate	Site	Source
Q14289-2 (PTK2B)	Y864	Uniprot
Q14289-1 (PTK2B)	Y906	Uniprot

Research Backgrounds

Q06124_PTN11_HUMAN

Function:

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73.

PTMs:

Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Widely expressed, with highest levels in heart, brain, and skeletal muscle.

Subunit Structure:

Interacts with phosphorylated LIME1 and BCAR3. Interacts with SHB and INPP5D/SHIP1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated). Interacts with FLT1 (tyrosine-phosphorylated), FLT3 (tyrosine-phosphorylated), FLT4 (tyrosine-phosphorylated), KIT and GRB2. Interacts with PDGFRA (tyrosine phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity). Interacts with PTPNS1 and CD84. Interacts with phosphorylated SIT1 and MPZL1. Interacts with FCRL4, FCRL6 and ANKHD1. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts with GAB2. Interacts with TERT; the interaction retains TERT in the nucleus. Interacts with PECAM1 and FER. Interacts with EPHA2 (activated); participates in PTK2/FAK1 dephosphorylation in EPHA2 downstream signaling. Interacts with ROS1; mediates PTPN11 phosphorylation. Interacts with PDGFRB (tyrosine phosphorylated); this interaction increases the PTPN11 phosphatase activity. Interacts with GAREM1 isoform 1 (tyrosine phosphorylated); the interaction increases MAPK/ERK activity and does not affect the GRB2/SOS complex formation. Interacts with CDC73. Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity). Interacts with MPIG6B (via ITIM motif). Interacts with SIGLEC10 (By similarity). Interacts with FCRL3 (via phosphorylated ITIM motifs).

Family&Domains:

The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

References

1). Isotoosendanin exerts anti-tumor effects in NSCLC by enhancing the stability of SHP-2 and inhibiting the JAK/STAT3 pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38924928) [IF=6.7]

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Phospho-SHP2 (Tyr580)[Tyr584] Antibody - #AF3413