Phospho-SHP2 (Tyr542)[Tyr546] Antibody - #AF3412

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Product: Phospho-SHP2 (Tyr542)[Tyr546] Antibody
Catalog: AF3412
Description: Rabbit polyclonal antibody to Phospho-SHP2 (Tyr542)[Tyr546]
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 70kDa; 68kD(Calculated).
Uniprot: Q06124
RRID: AB_2834839

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-SHP2 (Tyr542) Antibody detects endogenous levels of SHP2 only when phosphorylated at Tyr546, which site historically referenced as Tyr542.
RRID:
AB_2834839
Cite Format: Affinity Biosciences Cat# AF3412, RRID:AB_2834839.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BPTP3; CFC; JMML; METCDS; MGC14433; NS1; OTTHUMP00000166107; OTTHUMP00000166108; Protein tyrosine phosphatase 2; Protein tyrosine phosphatase 2C; Protein tyrosine phosphatase non receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTN11_HUMAN; PTP-1D; PTP-2C; PTP1D; PTP2C; PTPN11; SAP2; SH-PTP2; SH-PTP3; SH2 domain containing protein tyrosine phosphatase 2; SHP 2; SHP-2; Shp2; SHPTP2; SHPTP3; Syp; Tyrosine-protein phosphatase non-receptor type 11;

Immunogens

Immunogen:
Uniprot:

Q06124(PTN11_HUMAN) >>Visit HPA database.

Gene(ID):
PTPN11(5781)
Expression:
Q06124 PTN11_HUMAN:

Widely expressed, with highest levels in heart, brain, and skeletal muscle.

Description:
SHP-2 a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death.
Sequence:
MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q06124 As Substrate

Site PTM Type Enzyme
T2 Acetylation
S28 Phosphorylation
S34 Phosphorylation
S36 Phosphorylation
T59 Phosphorylation
Y62 Phosphorylation
Y63 Phosphorylation
Y66 Phosphorylation
T73 Phosphorylation
Y80 Phosphorylation
K120 Ubiquitination
K124 Ubiquitination
K131 Ubiquitination
S142 Phosphorylation
T153 Phosphorylation
S165 Phosphorylation
K178 Acetylation
Y179 Phosphorylation
S189 Phosphorylation
K198 Acetylation
K198 Ubiquitination
K235 Ubiquitination
K242 Ubiquitination
K244 Ubiquitination
K260 Ubiquitination
Y263 Phosphorylation
S264 Phosphorylation
K274 Acetylation
K276 Acetylation
Y279 Phosphorylation
K280 Acetylation
K280 Ubiquitination
S302 Phosphorylation
Y304 Phosphorylation
K317 Ubiquitination
S326 Phosphorylation
Y327 Phosphorylation
K389 Ubiquitination
T415 Phosphorylation
K486 Acetylation
Y515 Phosphorylation
Y519 Phosphorylation
S539 Phosphorylation
K542 Ubiquitination
Y546 Phosphorylation P08922 (ROS1) , P09619 (PDGFRB) , P06241 (FYN) , Q9UM73 (ALK)
T547 Phosphorylation
Y551 Phosphorylation
S552 Phosphorylation
T557 Phosphorylation
S558 Phosphorylation
S562 Phosphorylation
T568 Phosphorylation
T570 Phosphorylation
S580 Phosphorylation P17252 (PRKCA) , P05771 (PRKCB) , P24723 (PRKCH)
Y584 Phosphorylation P08922 (ROS1) , P00519 (ABL1) , Q9UM73 (ALK) , P42684 (ABL2)
K594 Ubiquitination
S595 Phosphorylation P05771-2 (PRKCB) , P24723 (PRKCH) , P17252 (PRKCA)

PTMs - Q06124 As Enzyme

Substrate Site Source
Q14289-2 (PTK2B) Y864 Uniprot
Q14289-1 (PTK2B) Y906 Uniprot

Research Backgrounds

Function:

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73.

PTMs:

Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with highest levels in heart, brain, and skeletal muscle.

Subunit Structure:

Interacts with phosphorylated LIME1 and BCAR3. Interacts with SHB and INPP5D/SHIP1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated). Interacts with FLT1 (tyrosine-phosphorylated), FLT3 (tyrosine-phosphorylated), FLT4 (tyrosine-phosphorylated), KIT and GRB2. Interacts with PDGFRA (tyrosine phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity). Interacts with PTPNS1 and CD84. Interacts with phosphorylated SIT1 and MPZL1. Interacts with FCRL4, FCRL6 and ANKHD1. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts with GAB2. Interacts with TERT; the interaction retains TERT in the nucleus. Interacts with PECAM1 and FER. Interacts with EPHA2 (activated); participates in PTK2/FAK1 dephosphorylation in EPHA2 downstream signaling. Interacts with ROS1; mediates PTPN11 phosphorylation. Interacts with PDGFRB (tyrosine phosphorylated); this interaction increases the PTPN11 phosphatase activity. Interacts with GAREM1 isoform 1 (tyrosine phosphorylated); the interaction increases MAPK/ERK activity and does not affect the GRB2/SOS complex formation. Interacts with CDC73. Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity). Interacts with MPIG6B (via ITIM motif). Interacts with SIGLEC10 (By similarity). Interacts with FCRL3 (via phosphorylated ITIM motifs).

Family&Domains:

The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

References

1). Acute stimulatory effect of tumor necrosis factor on the basolateral 50 pS K channels in the thick ascending limb of the rat kidney. Molecular Medicine Reports, 2018 (PubMed: 30221721) [IF=3.4]

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