CSF2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from RAW264.

Product:	CSF2 Antibody
Catalog:	DF12537
Description:	Rabbit polyclonal antibody to CSF2
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Rabbit, Dog
Mol.Wt.:	22 kDa; 16kD(Calculated).
Uniprot:	P04141
RRID:	AB_2845499

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(88%), Horse(88%), Rabbit(88%), Dog(100%)

Clonality:

Polyclonal

Specificity:

CSF2 Antibody detects endogenous levels of total CSF2.

RRID:

AB_2845499
Cite Format: Affinity Biosciences Cat# DF12537, RRID:AB_2845499.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Colony stimulating factor 2 (granulocyte-macrophage); Colony Stimulating Factor 2; Colony stimulating factor; Colony-stimulating factor; CSF 2; CSF; CSF2; CSF2_HUMAN; GM-CSF; GMCSF; Granulocyte Macrophage Colony Stimulating Factor; Granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGI1GM; Molgramostin; Pluripoietin-a; Sargramostim;

Immunogens

Immunogen:

Uniprot:

P04141(CSF2_HUMAN) >>Visit HPA database.

Gene(ID):

CSF2(1437)

Sequence:

P04141 CSF2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWLQSLLLLGTVACSISAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Horse

Bovine

Rabbit

Sheep

Pig

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04141 As Substrate

P04141

Site	PTM Type	Source
S22	O-Glycosylation	Uniprot
S24	O-Glycosylation	Uniprot
S26	O-Glycosylation	Uniprot
T27	O-Glycosylation	Uniprot
S86	Phosphorylation	Uniprot
T95	Phosphorylation	Uniprot
T119	Phosphorylation	Uniprot

Research Backgrounds

P04141_CSF2_HUMAN

Function:

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subcellular Location:

Secreted.

Subunit Structure:

Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits.

Family&Domains:

Belongs to the GM-CSF family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CSF2 Antibody - #DF12537