M-CSF Antibody | Affinity Biosciences

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Western blot analysis of extracts from U-937 cells, using CSF1 Antibody.

Product:	M-CSF Antibody
Catalog:	DF12536
Description:	Rabbit polyclonal antibody to M-CSF
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Sheep, Rabbit
Mol.Wt.:	70 kDa; 60kD(Calculated).
Uniprot:	P09603
RRID:	AB_2845498

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(92%), Bovine(100%), Sheep(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

M-CSF Antibody detects endogenous levels of total M-CSF.

RRID:

AB_2845498
Cite Format: Affinity Biosciences Cat# DF12536, RRID:AB_2845498.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Colony stimulating factor 1 (macrophage); Colony stimulating factor 1; Colony stimulating factor macrophage specific; CSF 1; CSF-1; CSF1; CSF1_HUMAN; Csfm; Lanimostim; M CSF; M-CSF; Macrophage Colony Stimulating Factor 1; Macrophage colony stimulating factor; MCSF; MGC31930; Processed macrophage colony-stimulating factor 1;

Immunogens

Immunogen:

Uniprot:

P09603(CSF1_HUMAN) >>Visit HPA database.

Gene(ID):

CSF1(1435)

Sequence:

P09603 CSF1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTAPGAAGRCPPTTWLGSLLLLVCLLASRSITEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQDVVTKPDCNCLYPKAIPSSDPASVSPHQPLAPSMAPVAGLTWEDSEGTEGSSLLPGEQPLHTVDPGSAKQRPPRSTCQSFEPPETPVVKDSTIGGSPQPRPSVGAFNPGMEDILDSAMGTNWVPEEASGEASEIPVPQGTELSPSRPGGGSMQTEPARPSNFLSASSPLPASAKGQQPADVTGTALPRVGPVRPTGQDWNHTPQKTDHPSALLRDPPEPGSPRISSLRPQGLSNPSTLSAQPQLSRSHSSGSVLPLGELEGRRSTRDRRSPAEPEGGPASEGAARPLPRFNSVPLTDTGHERQSEGSFSPQLQESVFHLLVPSVILVLLAVGGLLFYRWRRRSHQEPQRADSPLEQPEGSPLTQDDRQVELPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Rabbit

100

Pig

Horse

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09603 As Substrate

P09603

Site	PTM Type	Source
S256	O-Glycosylation	Uniprot
T257	O-Glycosylation	Uniprot
T266	O-Glycosylation	Uniprot
T266	Phosphorylation	Uniprot
S345	Phosphorylation	Uniprot
S348	Phosphorylation	Uniprot
S353	Phosphorylation	Uniprot
T363	O-Glycosylation	Uniprot
T365	O-Glycosylation	Uniprot
T376	O-Glycosylation	Uniprot
T383	O-Glycosylation	Uniprot
T387	O-Glycosylation	Uniprot
S391	O-Glycosylation	Uniprot
S420	Phosphorylation	Uniprot
S426	Phosphorylation	Uniprot
S428	O-Glycosylation	Uniprot
S430	O-Glycosylation	Uniprot
S431	O-Glycosylation	Uniprot
S433	O-Glycosylation	Uniprot
S451	O-Glycosylation	Uniprot
S461	O-Glycosylation	Uniprot
S461	Phosphorylation	Uniprot
S473	O-Glycosylation	Uniprot
T477	O-Glycosylation	Uniprot
S533	Phosphorylation	Uniprot

Research Backgrounds

P09603_CSF1_HUMAN

Function:

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

PTMs:

N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted>Extracellular space.

Subunit Structure:

Homodimer or heterodimer; disulfide-linked. Interacts with CSF1R.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

References

1). Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis. European Journal of Histochemistry, 2023 (PubMed: 37491944) [IF=2.0]

Application: WB Species: Human Sample: SaOS-2 cells

Figure 4. Deletion of osteopontin (OPN) promoted the differentiation and mineralization of osteoblasts. A) Osteocalcin-positive rate of SaOS-2 cells was detected by flow cytometry. B) The expression of osteocalcin in SaOS-2 cells was observed by immunofluorescence staining, and the fluorescence intensity was exhibited on the right. C) Alizarin Red S staining was performed on SaOS-2 cells to evaluate the mineralization. D) The protein expression of RANKL, M-CSF and OPG was detected by Western blot.

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M-CSF Antibody - #DF12536