Product: PERM Antibody
Catalog: DF12528
Description: Rabbit polyclonal antibody to PERM
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 84 kDa; 84kD(Calculated).
Uniprot: P05164
RRID: AB_2845490

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(91%), Bovine(91%), Sheep(91%), Rabbit(91%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
PERM Antibody detects endogenous levels of total PERM.
RRID:
AB_2845490
Cite Format: Affinity Biosciences Cat# DF12528, RRID:AB_2845490.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

84 kDa myeloperoxidase; 89 kDa myeloperoxidase; EC 1.11.1.7; EC1.11.2.2; fj80f04; MPO; mpx; myeloid-specific peroxidase; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain; PERM_HUMAN; wu:fj80f04;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MGVPFFSSLRCMVDLGPCWAGGLTAEMKLLLALAGLLAILATPQPSEGAAPAVLGEVDTSLVLSSMEEAKQLVDKAYKERRESIKQRLRSGSASPMELLSYFKQPVAATRTAVRAADYLHVALDLLERKLRSLWRRPFNVTDVLTPAQLNVLSKSSGCAYQDVGVTCPEQDKYRTITGMCNNRRSPTLGASNRAFVRWLPAEYEDGFSLPYGWTPGVKRNGFPVALARAVSNEIVRFPTDQLTPDQERSLMFMQWGQLLDHDLDFTPEPAARASFVTGVNCETSCVQQPPCFPLKIPPNDPRIKNQADCIPFFRSCPACPGSNITIRNQINALTSFVDASMVYGSEEPLARNLRNMSNQLGLLAVNQRFQDNGRALLPFDNLHDDPCLLTNRSARIPCFLAGDTRSSEMPELTSMHTLLLREHNRLATELKSLNPRWDGERLYQEARKIVGAMVQIITYRDYLPLVLGPTAMRKYLPTYRSYNDSVDPRIANVFTNAFRYGHTLIQPFMFRLDNRYQPMEPNPRVPLSRVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIAVDEIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPETVGQLGTVLRNLKLARKLMEQYGTPNNIDIWMGGVSEPLKRKGRVGPLLACIIGTQFRKLRDGDRFWWENEGVFSMQQRQALAQISLPRIICDNTGITTVSKNNIFMSNSYPRDFVNCSTLPALNLASWREAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
91
Bovine
91
Sheep
91
Rabbit
91
Xenopus
80
Horse
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05164 As Substrate

Site PTM Type Enzyme
K103 Acetylation
N139 N-Glycosylation
S191 Phosphorylation
N323 N-Glycosylation
N355 N-Glycosylation
S357 Phosphorylation
N391 N-Glycosylation
T428 Phosphorylation
S432 Phosphorylation
Y500 Phosphorylation
Y516 Phosphorylation
S528 Phosphorylation
K625 Sumoylation
S720 Phosphorylation
Y723 Phosphorylation
N729 N-Glycosylation

Research Backgrounds

Function:

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Subcellular Location:

Lysosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain.

Family&Domains:

Belongs to the peroxidase family. XPO subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

References

1). Liver proteomic analysis reveals acute liver failure induced by lipopolysaccharide/D-galactosamine in rats involved in neutrophil extracellular trap formation. European Journal of Inflammation, 2022 [IF=0.7]

Application: WB    Species: Rat    Sample: Liver

Figure 4. Lipopolysaccharide (LPS)/D-galactosamine (D-Gal)-induced acute liver failure (ALF) in rats involved in neutrophil extracellular trap (NET) formation. (a) Validation of selected differentially expressed proteins of cathelicidin antimicrobial peptide (CAMP), myeloperoxidase (MPO), and fibrinogen gamma chain (FGG) in the liver samples ofrats after LPS/D-Gal administration for different time periods using western blotting analysis in the validation cohort. (b) Serum MPO-DNA levels, (c) citrullination of histone H3 (Cit-H3) levels, (d) tumour necrosis factor-α (TNF-α) levels, and (e) interleukin-6 (IL-6) levels were measured in rats with LPS/D-Gal administration for different time periods, respectively.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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