PERM Antibody | Affinity Biosciences

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Western blot analysis of extracts from HL-60 cells, using PERM Antibody.

Product:	PERM Antibody
Catalog:	DF12528
Description:	Rabbit polyclonal antibody to PERM
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	84 kDa; 84kD(Calculated).
Uniprot:	P05164
RRID:	AB_2845490

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Bovine(91%), Sheep(91%), Rabbit(91%), Dog(100%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

PERM Antibody detects endogenous levels of total PERM.

RRID:

AB_2845490
Cite Format: Affinity Biosciences Cat# DF12528, RRID:AB_2845490.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

84 kDa myeloperoxidase; 89 kDa myeloperoxidase; EC 1.11.1.7; EC1.11.2.2; fj80f04; MPO; mpx; myeloid-specific peroxidase; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain; PERM_HUMAN; wu:fj80f04;

Immunogens

Immunogen:

Uniprot:

P05164(PERM_HUMAN) >>Visit HPA database.

Gene(ID):

MPO(4353)

Sequence:

P05164 PERM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGVPFFSSLRCMVDLGPCWAGGLTAEMKLLLALAGLLAILATPQPSEGAAPAVLGEVDTSLVLSSMEEAKQLVDKAYKERRESIKQRLRSGSASPMELLSYFKQPVAATRTAVRAADYLHVALDLLERKLRSLWRRPFNVTDVLTPAQLNVLSKSSGCAYQDVGVTCPEQDKYRTITGMCNNRRSPTLGASNRAFVRWLPAEYEDGFSLPYGWTPGVKRNGFPVALARAVSNEIVRFPTDQLTPDQERSLMFMQWGQLLDHDLDFTPEPAARASFVTGVNCETSCVQQPPCFPLKIPPNDPRIKNQADCIPFFRSCPACPGSNITIRNQINALTSFVDASMVYGSEEPLARNLRNMSNQLGLLAVNQRFQDNGRALLPFDNLHDDPCLLTNRSARIPCFLAGDTRSSEMPELTSMHTLLLREHNRLATELKSLNPRWDGERLYQEARKIVGAMVQIITYRDYLPLVLGPTAMRKYLPTYRSYNDSVDPRIANVFTNAFRYGHTLIQPFMFRLDNRYQPMEPNPRVPLSRVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIAVDEIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPETVGQLGTVLRNLKLARKLMEQYGTPNNIDIWMGGVSEPLKRKGRVGPLLACIIGTQFRKLRDGDRFWWENEGVFSMQQRQALAQISLPRIICDNTGITTVSKNNIFMSNSYPRDFVNCSTLPALNLASWREAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Bovine

Sheep

Rabbit

Xenopus

Horse

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05164 As Substrate

P05164

Site	PTM Type	Source
K103	Acetylation	Uniprot
N139	N-Glycosylation	Uniprot
S191	Phosphorylation	Uniprot
N323	N-Glycosylation	Uniprot
N355	N-Glycosylation	Uniprot
S357	Phosphorylation	Uniprot
N391	N-Glycosylation	Uniprot
T428	Phosphorylation	Uniprot
S432	Phosphorylation	Uniprot
Y500	Phosphorylation	Uniprot
Y516	Phosphorylation	Uniprot
S528	Phosphorylation	Uniprot
K625	Sumoylation	Uniprot
S720	Phosphorylation	Uniprot
Y723	Phosphorylation	Uniprot
N729	N-Glycosylation	Uniprot

Research Backgrounds

P05164_PERM_HUMAN

Function:

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Subcellular Location:

Lysosome.

Subunit Structure:

Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain.

Family&Domains:

Belongs to the peroxidase family. XPO subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

References

1). Liver proteomic analysis reveals acute liver failure induced by lipopolysaccharide/D-galactosamine in rats involved in neutrophil extracellular trap formation. European Journal of Inflammation [IF=0.7]

Application: WB Species: Rat Sample: Liver

Figure 4. Lipopolysaccharide (LPS)/D-galactosamine (D-Gal)-induced acute liver failure (ALF) in rats involved in neutrophil extracellular trap (NET) formation. (a) Validation of selected differentially expressed proteins of cathelicidin antimicrobial peptide (CAMP), myeloperoxidase (MPO), and fibrinogen gamma chain (FGG) in the liver samples ofrats after LPS/D-Gal administration for different time periods using western blotting analysis in the validation cohort. (b) Serum MPO-DNA levels, (c) citrullination of histone H3 (Cit-H3) levels, (d) tumour necrosis factor-α (TNF-α) levels, and (e) interleukin-6 (IL-6) levels were measured in rats with LPS/D-Gal administration for different time periods, respectively.

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PERM Antibody - #DF12528