MMP24 Antibody - #DF12527

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Product: MMP24 Antibody
Catalog: DF12527
Description: Rabbit polyclonal antibody to MMP24
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 50 kDa; 73kD(Calculated).
Uniprot: Q9Y5R2
RRID: AB_2845489

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
MMP24 Antibody detects endogenous levels of total MMP24.
RRID:
AB_2845489
Cite Format: Affinity Biosciences Cat# DF12527, RRID:AB_2845489.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Matrix metallopeptidase 24 (membrane inserted); Matrix metallopeptidase 24; Membrane type 5 matrix metalloproteinase; Membrane type matrix metalloproteinase 5; Membrane-type matrix metalloproteinase 5; Membrane-type-5 matrix metalloproteinase; MMP 24; MMP 25; MMP-24; Mmp24; MMP24_HUMAN; MMP25; MT MMP5; MT-MMP 5; MT5 MMP; MT5-MMP; MT5MMP; MTMMP5; Processed matrix metalloproteinase-24;

Immunogens

Immunogen:
Uniprot:

Q9Y5R2(MMP24_HUMAN) >>Visit HPA database.

Gene(ID):
MMP24(10893)
Expression:
Q9Y5R2 MMP24_HUMAN:

Predominantly expressed in brain, kidney, pancreas and lung. Overexpressed in a series of brain tumors, including astrocytomas and glioblastomas.

Sequence:
MPRSRGGRAAPGPPPPPPPPGQAPRWSRWRVPGRLLLLLLPALCCLPGAARAAAAAAGAGNRAAVAVAVARADEAEAPFAGQNWLKSYGYLLPYDSRASALHSAKALQSAVSTMQQFYGIPVTGVLDQTTIEWMKKPRCGVPDHPHLSRRRRNKRYALTGQKWRQKHITYSIHNYTPKVGELDTRKAIRQAFDVWQKVTPLTFEEVPYHEIKSDRKEADIMIFFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNANHDGNDLFLVAVHELGHALGLEHSSDPSAIMAPFYQYMETHNFKLPQDDLQGIQKIYGPPAEPLEPTRPLPTLPVRRIHSPSERKHERQPRPPRPPLGDRPSTPGTKPNICDGNFNTVALFRGEMFVFKDRWFWRLRNNRVQEGYPMQIEQFWKGLPARIDAAYERADGRFVFFKGDKYWVFKEVTVEPGYPHSLGELGSCLPREGIDTALRWEPVGKTYFFKGERYWRYSEERRATDPGYPKPITVWKGIPQAPQGAFISKEGYYTYFYKGRDYWKFDNQKLSVEPGYPRNILRDWMGCNQKEVERRKERRLPQDDVDIMVTINDVPGSVNAVAVVIPCILSLCILVLVYTIFQFKNKTGPQPVTYYKRPVQEWV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
82
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5R2 As Substrate

Site PTM Type Enzyme
R8 Methylation
Y175 Phosphorylation
T176 Phosphorylation
Y534 Phosphorylation
Y537 Phosphorylation
T629 Phosphorylation
Y637 Phosphorylation

Research Backgrounds

Function:

Metalloprotease that mediates cleavage of N-cadherin (CDH2) and acts as a regulator of neuro-immune interactions and neural stem cell quiescence. Involved in cell-cell interactions between nociceptive neurites and mast cells, possibly by mediating cleavage of CDH2, thereby acting as a mediator of peripheral thermal nociception and inflammatory hyperalgesia. Key regulator of neural stem cells quiescence by mediating cleavage of CDH2, affecting CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate their quiescence. May play a role in axonal growth. Able to activate progelatinase A. May also be a proteoglycanase involved in degradation of proteoglycans, such as dermatan sulfate and chondroitin sulfate proteoglycans. Cleaves partially fibronectin, but not collagen type I, nor laminin (By similarity).

PTMs:

Cleaved by a furin endopeptidase in the trans-Golgi network.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Golgi apparatus>trans-Golgi network membrane>Single-pass type I membrane protein.
Note: Recycled back to the plasma membrane through the trans-Golgi network via interaction with APBA3.

Secreted>Extracellular space>Extracellular matrix.
Note: Also shed from cell surface as soluble proteinase, by a proteolytic cleavage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in brain, kidney, pancreas and lung. Overexpressed in a series of brain tumors, including astrocytomas and glioblastomas.

Subunit Structure:

Interacts (via PDZ-binding motif) with APBA3 (via PDZ domain). Interacts with GRIP1 and GRIP2 (By similarity).

Family&Domains:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

The PDZ-binding motif (also named EWV motif) is required for interaction with PDZ domains of APBA3 and recycling through the trans-Golgi network.

Belongs to the peptidase M10A family.

References

1). MMP24 Contributes to Neuropathic Pain in an FTO-Dependent Manner in the Spinal Cord Neurons. Frontiers in Pharmacology, 2021 (PubMed: 33995105) [IF=5.6]

Application: IF/ICC    Species: mouse    Sample: spinal cord

FIGURE1 | (D–F). Representative images of MMP24 immunofluorescence in the L4 dorsal horn. MMP24 immunoreactivity was low in naïve mice, but increased in the ipsilateral dorsal horn compared with the contralateral dorsal horn 7 days after SNL. Scale bar: 50 μm; 25 μm (insets).

Application: WB    Species: mouse    Sample: spinal cord

FIGURE1 |(C). Expression of MMP24 protein in the spinal cord after sham surgery. n 3 mice/time point.

Application: WB    Species: Mice    Sample: spinal cord cells

FIGURE 1 MMP24 protein is increased in the spinal cord after SNL (A). Expression of Mmp24 mRNA after SNL. n = 4 mice/group/time point. (B). Expression of MMP24 protein in the spinal cord after SNL surgery. n = 3–6 mice/time point. One-way ANOVA followed by post hoc Tukey test. Ftime (3, 8) = 29.00. * p < 0.05, ** p < 0.01 vs. the corresponding control group (0 day). (C). Expression of MMP24 protein in the spinal cord after sham surgery. n = 3 mice/time point. (D–F). Representative images of MMP24 immunofluorescence in the L4 dorsal horn. MMP24 immunoreactivity was low in naïve mice, but increased in the ipsilateral dorsal horn compared with the contralateral dorsal horn 7 days after SNL. Scale bar: 50 μm; 25 μm (insets). (G–K). Double staining of MMP24 and markers of neuron, astrocytes, and microglia on day 14 after SNL. Scale bar: 50 μm.

Application: IF/ICC    Species: Mice    Sample: spinal cord cells

FIGURE 1 MMP24 protein is increased in the spinal cord after SNL (A). Expression of Mmp24 mRNA after SNL. n = 4 mice/group/time point. (B). Expression of MMP24 protein in the spinal cord after SNL surgery. n = 3–6 mice/time point. One-way ANOVA followed by post hoc Tukey test. Ftime (3, 8) = 29.00. * p < 0.05, ** p < 0.01 vs. the corresponding control group (0 day). (C). Expression of MMP24 protein in the spinal cord after sham surgery. n = 3 mice/time point. (D–F). Representative images of MMP24 immunofluorescence in the L4 dorsal horn. MMP24 immunoreactivity was low in naïve mice, but increased in the ipsilateral dorsal horn compared with the contralateral dorsal horn 7 days after SNL. Scale bar: 50 μm; 25 μm (insets). (G–K). Double staining of MMP24 and markers of neuron, astrocytes, and microglia on day 14 after SNL. Scale bar: 50 μm.
2). PCSK6 exacerbates Alzheimer's disease pathogenesis by promoting MT5-MMP maturation. Experimental neurology, 2024 (PubMed: 38216110) [IF=4.6]

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