Product: MDR3 Antibody
Catalog: DF12513
Description: Rabbit polyclonal antibody to MDR3
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Rabbit, Dog
Mol.Wt.: 142 kDa; 142kD(Calculated).
Uniprot: P21439
RRID: AB_2845475

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MDR3 Antibody detects endogenous levels of total MDR3.
RRID:
AB_2845475
Cite Format: Affinity Biosciences Cat# DF12513, RRID:AB_2845475.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABC 21; ABC B4; ABC21; ABCB 4; Abcb4; ABCB4 protein; ATP binding cassette sub family B MDR/TAP member 4; ATP binding cassette sub family B member 4; ATP-binding cassette sub-family B member 4; GBD1; ICP3; MDR 3; MDR2; MDR2/3; MDR3; MDR3 P glycoprotein; MDR3 P gp; MDR3_HUMAN; Multidrug resistance protein 3; Multiple drug resistance 3; P glycoprotein 3; P-glycoprotein 3; PFIC 3; PFIC3; PGY 3; PGY3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MDLEAAKNGTAWRPTSAEGDFELGISSKQKRKKTKTVKMIGVLTLFRYSDWQDKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDKFVDTAGNFSFPVNFSLSLLNPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGNVTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNMQTSGSQIQSEEFELNDEKAATRMAPNGWKSRLFRHSTQKNLKNSQMCQKSLDVETDGLEANVPPVSFLKVLKLNKTEWPYFVVGTVCAIANGGLQPAFSVIFSEIIAIFGPGDDAVKQQKCNIFSLIFLFLGIISFFTFFLQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGHMRFRDVILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVFVDFGFQLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTQNL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Bovine
92
Zebrafish
73
Sheep
70
Horse
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P21439 As Substrate

Site PTM Type Enzyme
T34 Phosphorylation
S121 Phosphorylation
K413 Ubiquitination
T424 Phosphorylation
T524 Phosphorylation
K538 Ubiquitination
Y624 Phosphorylation
K679 Acetylation
K699 Acetylation
K1061 Ubiquitination
K1178 Ubiquitination
K1187 Ubiquitination

Research Backgrounds

Function:

Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts. Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes. Required for proper phospholipid bile formation (By similarity). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner. Promotes biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane. In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts. Does not confer multidrug resistance (By similarity).

PTMs:

Phosphorylated. Phosphorylation on Thr-34 is required for PC efflux activity. Phosphorylation occurs on serine and threonine residues in a protein kinase A- or C-dependent manner. May be phosphorylated on Thr-44 and Ser-49.

Glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Membrane raft. Cytoplasm. Cytoplasmic vesicle>Clathrin-coated vesicle.
Note: Localized at the apical canalicular membrane of the epithelial cells lining the lumen of the bile canaliculi and biliary ductules (By similarity). Transported from the Golgi to the apical bile canalicular membrane in a RACK1-dependent manner (PubMed:19674157). Redistributed into pseudocanaliculi formed between cells in a bezafibrate- or PPARA-dependent manner (PubMed:15258199). Localized preferentially in lipid nonraft domains of canalicular plasma membranes (PubMed:23468132).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

May interact with RACK1. Interacts with HAX1 (By similarity).

Family&Domains:

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

References

1). Apigenin protects mice against 3, 5-diethoxycarbonyl-1, 4-dihydrocollidine-induced cholestasis. Food & Function, 2021 (PubMed: 33620063) [IF=6.1]

Application: WB    Species: mice    Sample: liver tissues

Fig. 3 Apigenin blocks DDC-induced liver hypertrophy, gallbladder atrophy and bile anabolic abnormalities by regulating the FXR pathway. (A) At the end of experiment, liver and gallbladder samples were collected and photographed. (B) Serum and liver TBA levels were determined with an assay kit. (C and D) mRNA expression of ABCG5, BSEP and ABCB4 (C), and FXR, SHP and CYP8B1 (D) in the liver was determined by qRT-PCR. (E) Protein expression of ABCG5, BSEP, ABCB4, FXR, SHP and CYP7A1 in the liver was determined by Western blot, followed by quantitative analysis of band density. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (n ≥ 5); API: apigenin.

2). Novel chimeric TLR2/NOD2 agonist CL429 exhibited significant radioprotective effects in mice. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33609010) [IF=5.3]

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