KLF4 Antibody - #AF2737

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Product: KLF4 Antibody
Catalog: AF2737
Description: Rabbit polyclonal antibody to KLF4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit, Dog
Mol.Wt.: 50-60kDa; 55kD(Calculated).
Uniprot: O43474
RRID: AB_2845457

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
KLF4 Antibody detects endogenous levels of total KLF4.
RRID:
AB_2845457
Cite Format: Affinity Biosciences Cat# AF2737, RRID:AB_2845457.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Endothelial Kruppel like zinc finger protein; Epithelial zinc finger protein EZF; EZF; GKLF; gut Kruppel-like factor; Gut-enriched krueppel-like factor; KLF; KLF4; KLF4_HUMAN; Krueppel-like factor 4; Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor); Kruppel like factor 4 (gut);

Immunogens

Immunogen:
Uniprot:

O43474(KLF4_HUMAN) >>Visit HPA database.

Gene(ID):
KLF4(9314)
Sequence:
MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQGQSRGFVARAGEPCVCWPHFGTHGMMLTPPSSPLELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Pig
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43474 As Substrate

Site PTM Type Enzyme
K32 Ubiquitination
S234 Phosphorylation
S236 Phosphorylation
S240 Phosphorylation
S244 Phosphorylation
S254 Phosphorylation
S258 Phosphorylation
K278 Sumoylation
T316 Phosphorylation
S327 Phosphorylation
T429 Phosphorylation
S444 Phosphorylation
S445 Phosphorylation
K448 Sumoylation
K448 Ubiquitination
T453 Phosphorylation
T455 Phosphorylation

Research Backgrounds

Function:

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with POU5F1/OCT4 and SOX2 (By similarity). Interacts with MUC1 (via the C-terminal domain). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a. Interacts with ZNF296 (By similarity). Interacts with GLIS1.

Family&Domains:

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the krueppel C2H2-type zinc-finger protein family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

References

1). Artesunate inhibits macrophage-like phenotype switching of vascular smooth muscle cells and attenuates vascular inflammatory injury in atherosclerosis via NLRP3. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38325261) [IF=7.5]

Application: WB    Species: Human    Sample: HVSMCs

Fig. 7. Artesunate inhibits the inflammatory phenotypic transformation of HVSMCs and is related to NLRP3 inhibition. (A) Oil Red O staining of HVSMCs in vitro. (B) Immunofluorescence assay to determine the levels of α-SMA and CD68 in vitro. (C) Relative protein expression of KLF4 in vitro. (D) Immunofluorescence for α-SMA and CD68 expression in the aorta. (E) Immunohistochemistry for KLF4 expression in the aorta. ***P 
2). A Potent Protective Effect of Baicalein on Liver Injury by Regulating Mitochondria-Related Apoptosis. APOPTOSIS, 2020 (PubMed: 32409930) [IF=7.2]

Application: WB    Species: human    Sample: L02 cells

Fig. 5 |The protective mechanism of baicalein on oxidative stress induced hepatotoxicity. L02 cells were exposed to 100 μM H­2O2 for diferent indicated time. a The expression level of Drp1 and KLF4 were detected by western blotting and densitometry.
3). Succinate pretreatment attenuates intestinal ischemia-reperfusion injury by inhibiting necroptosis and inflammation via upregulating Klf4. International Immunopharmacology, 2023 (PubMed: 37285681) [IF=5.6]
4). Baicalein inhibits mitochondrial apoptosis induced by oxidative stress in cardiomyocytes by stabilizing MARCH5 expression. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020 (PubMed: 31880404) [IF=5.3]

Application: WB    Species: mouse    Sample: H9C2 cells

FIGURE 6|The cardioprotective pathway regulated by baicalein. H9C2 cells were exposed to 100μM H2O2 for 24 h after pre-treated with 50 μM baicalein for 4 h, transfected with MARCH5-cDNA or MARCH5-siRNA for 24 h, respectively. KLF4 and Drp1 proteins were detected by Western blotting. (A) The expression level of KLF4 and Drp1 in different time groups after treatment with H2O2

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