Phospho-Calnexin (Ser564) Antibody - #AF2438

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Product: Phospho-Calnexin (Ser564) Antibody
Catalog: AF2438
Description: Rabbit polyclonal antibody to Phospho-Calnexin (Ser564)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 90kDa; 68kD(Calculated).
Uniprot: P27824
RRID: AB_2845451

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(83%), Horse(83%), Sheep(92%), Rabbit(100%), Dog(83%)
Clonality:
Polyclonal
Specificity:
Phospho-Calnexin (Ser564) Antibody detects endogenous levels of Calnexin only when phosphorylated at Ser564.
RRID:
AB_2845451
Cite Format: Affinity Biosciences Cat# AF2438, RRID:AB_2845451.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Calnexin; CALX_HUMAN; CANX; CNX; FLJ26570; Histocompatibility complex class I antigen binding protein p88; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90;

Immunogens

Immunogen:
Uniprot:

P27824(CALX_HUMAN) >>Visit HPA database.

Gene(ID):
CANX(821)
Sequence:
MEGKWLLCMLLVLGTAIVEAHDGHDDDVIDIEDDLDDVIEEVEDSKPDTTAPPSSPKVTYKAPVPTGEVYFADSFDRGTLSGWILSKAKKDDTDDEIAKYDGKWEVEEMKESKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTPELNLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGIYEEKHAKRPDADLKTYFTDKKTHLYTLILNPDNSFEILVDQSVVNSGNLLNDMTPPVNPSREIEDPEDRKPEDWDERPKIPDPEAVKPDDWDEDAPAKIPDEEATKPEGWLDDEPEYVPDPDAEKPEDWDEDMDGEWEAPQIANPRCESAPGCGVWQRPVIDNPNYKGKWKPPMIDNPSYQGIWKPRKIPNPDFFEDLEPFRMTPFSAIGLELWSMTSDIFFDNFIICADRRIVDDWANDGWGLKKAADGAAEPGVVGQMIEAAEERPWLWVVYILTVALPVFLVILFCCSGKKQTSGMEYKKTDAPQPDVKEEEEEKEEEKDKGDEEEEGEEKLEEKQKSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Sheep
92
Horse
83
Bovine
83
Dog
83
Chicken
57
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P27824 As Substrate

Site PTM Type Enzyme
T59 O-Glycosylation
K61 Ubiquitination
T66 O-Glycosylation
Y70 Phosphorylation
S74 Phosphorylation
R77 Methylation
T79 O-Glycosylation
S81 Phosphorylation
S86 Phosphorylation
K87 Ubiquitination
T93 Phosphorylation
K99 Ubiquitination
K103 Acetylation
K103 Ubiquitination
K110 Ubiquitination
K118 Ubiquitination
R125 Methylation
K127 Ubiquitination
K134 Acetylation
K134 Ubiquitination
K137 Acetylation
K137 Ubiquitination
K170 Ubiquitination
K182 Ubiquitination
Y185 Phosphorylation
K193 Ubiquitination
K199 Acetylation
K199 Ubiquitination
K210 Ubiquitination
T211 Phosphorylation
Y214 Phosphorylation
K217 Acetylation
K217 Ubiquitination
K227 Acetylation
K227 Ubiquitination
T228 Phosphorylation
K233 Ubiquitination
R282 Methylation
K283 Ubiquitination
K300 Ubiquitination
S362 Phosphorylation
Y379 Phosphorylation
K380 Ubiquitination
Y393 Phosphorylation
K398 Ubiquitination
K401 Ubiquitination
S431 Phosphorylation
K458 Acetylation
K458 Ubiquitination
K459 Acetylation
K459 Ubiquitination
T509 Phosphorylation
S510 Phosphorylation
Y514 Phosphorylation
K516 Ubiquitination
K525 Ubiquitination
K537 Ubiquitination
K547 Ubiquitination
S554 Phosphorylation
T562 Phosphorylation
S564 Phosphorylation
S583 Phosphorylation P27361 (MAPK3) , P06493 (CDK1)

Research Backgrounds

Function:

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

PTMs:

Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).

Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins.

Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with MAPK3/ERK1 (By similarity). Interacts with KCNH2. Associates with ribosomes (By similarity). Interacts with SGIP1; involved in negative regulation of endocytosis (By similarity). The palmitoylated form interacts with the ribosome-translocon complex component SSR1, promoting efficient folding of glycoproteins. Interacts with SERPINA2P/SERPINA2 and with the S and Z variants of SERPINA1. Interacts with PPIB. Interacts with ZNRF4. Interacts with SMIM22.

(Microbial infection) Interacts with HBV large envelope protein, isoform L.

(Microbial infection) Interacts with HBV large envelope protein, isoform M; this association may be essential for isoform M proper secretion.

Family&Domains:

Belongs to the calreticulin family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

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