Phospho-WIPI2 (Ser413) Antibody | Affinity Biosciences

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Product:	Phospho-WIPI2 (Ser413) Antibody
Catalog:	AF2434
Description:	Rabbit polyclonal antibody to Phospho-WIPI2 (Ser413)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	49kDa; 49kD(Calculated).
Uniprot:	Q9Y4P8
RRID:	AB_2845447

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Sheep(82%), Rabbit(91%), Dog(92%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

Phospho-WIPI2 (Ser413) Antibody detects endogenous levels of WIPI2 only when phosphorylated at Ser413.

RRID:

AB_2845447
Cite Format: Affinity Biosciences Cat# AF2434, RRID:AB_2845447.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATG18B; Atg21; CGI 50; DKFZp434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain, phosphoinositide interacting 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI 2; WIPI-2; WIPI2; WIPI2_HUMAN; WIPI49 like protein 2; WIPI49-like protein 2;

Immunogens

Immunogen:

Uniprot:

Q9Y4P8(WIPI2_HUMAN) >>Visit HPA database.

Gene(ID):

WIPI2(26100)

Expression:

Q9Y4P8 WIPI2_HUMAN:

Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.

Sequence:

Q9Y4P8 WIPI2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNLASQSGEAGAGQLLFANFNQDNTEVKGASRAAGLGRRAVVWSLAVGSKSGYKFFSLSSVDKLEQIYECTDTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLETVKEKPPEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATIQKIPRLLVGAADGYLYMYNLDPQEGGECALMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKGTYVPSSPTRLAYTDDLGAVGGACLEDEASALRLDEDSEHPPMILRTD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Chicken

Rabbit

Pig

Sheep

Xenopus

Bovine

Horse

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y4P8 As Substrate

Q9Y4P8

Site	PTM Type	Source
K28	Ubiquitination	Uniprot
K146	Ubiquitination	Uniprot
K216	Ubiquitination	Uniprot
K223	Acetylation	Uniprot
K223	Ubiquitination	Uniprot
K237	Ubiquitination	Uniprot
K283	Ubiquitination	Uniprot
T292	Phosphorylation	Uniprot
S308	Phosphorylation	Uniprot
K331	Ubiquitination	Uniprot
K341	Ubiquitination	Uniprot
K371	Ubiquitination	Uniprot
S378	Phosphorylation	Uniprot
S412	Phosphorylation	Uniprot
S413	Phosphorylation	Uniprot
T415	Phosphorylation	Uniprot

Research Backgrounds

Q9Y4P8_WIPI2_HUMAN

Function:

Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.

Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella.

Subcellular Location:

Preautophagosomal structure membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Localizes to omegasomes membranes which are endoplasmic reticulum connected structures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.

Tissue Specificity:

Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.

Subunit Structure:

Interacts with TECPR1. Interacts with ATG16L1. Interacts with ATG5. Interacts with WIPI1. Interacts with WDR45. May interact with NUDC.

Family&Domains:

The FRRG motif is required for recruitment to PtdIns3P.

Belongs to the WD repeat SVP1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

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Phospho-WIPI2 (Ser413) Antibody - #AF2434