Phospho-TNK1 (Tyr277) Antibody - #AF2426

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Product: Phospho-TNK1 (Tyr277) Antibody
Catalog: AF2426
Description: Rabbit polyclonal antibody to Phospho-TNK1 (Tyr277)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 72kDa; 72kD(Calculated).
Uniprot: Q13470
RRID: AB_2845439

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-TNK1 (Tyr277) Antibody detects endogenous levels of TNK1 only when phosphorylated at Tyr277.
RRID:
AB_2845439
Cite Format: Affinity Biosciences Cat# AF2426, RRID:AB_2845439.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD38 negative kinase 1; EC 2.7.10.2; Kinase of embryonic stem cells; Kos 1; Kos1; MGC46193; Non receptor tyrosine protein kinase TNK 1; Non receptor tyrosine protein kinase TNK1; Non-receptor tyrosine-protein kinase TNK1; Tnk 1; Tnk1; TNK1_HUMAN; Tyrosine kinase non receptor 1;

Immunogens

Immunogen:
Uniprot:

Q13470(TNK1_HUMAN) >>Visit HPA database.

Gene(ID):
TNK1(8711)
Expression:
Q13470 TNK1_HUMAN:

Expressed in all umbilical cord blood, bone marrow and adult blood cell sub-populations and in several leukemia cell lines. Highly expressed in fetal blood, brain, lung, liver and kidney. Detected at lower levels in adult prostate, testis, ovary, small intestine and colon. Not expressed in adult lung, liver, kidney or brain.

Sequence:
MLPEAGSLWLLKLLRDIQLAQFYWPILEELNVTRPEHFDFVKPEDLDGIGMGRPAQRRLSEALKRLRSGPKSKNWVYKILGGFAPEHKEPTLPSDSPRHLPEPEGGLKCLIPEGAVCRGELLGSGCFGVVHRGLWTLPSGKSVPVAVKSLRVGPEGPMGTELGDFLREVSVMMNLEHPHVLRLHGLVLGQPLQMVMELAPLGSLHARLTAPAPTPPLLVALLCLFLRQLAGAMAYLGARGLVHRDLATRNLLLASPRTIKVADFGLVRPLGGARGRYVMGGPRPIPYAWCAPESLRHGAFSSASDVWMFGVTLWEMFSGGEEPWAGVPPYLILQRLEDRARLPRPPLCSRALYSLALRCWAPHPADRPSFSHLEGLLQEAGPSEACCVRDVTEPGALRMETGDPITVIEGSSSFHSPDSTIWKGQNGRTFKVGSFPASAVTLADAGGLPATRPVHRGTPARGDQHPGSIDGDRKKANLWDAPPARGQRRNMPLERMKGISRSLESVLSLGPRPTGGGSSPPEIRQARAVPQGPPGLPPRPPLSSSSPQPSQPSRERLPWPKRKPPHNHPMGMPGARKAAALSGGLLSDPELQRKIMEVELSVHGVTHQECQTALGATGGDVVSAIRNLKVDQLFHLSSRSRADCWRILEHYQWDLSAASRYVLARP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
92
Rabbit
92
Zebrafish
67
Sheep
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13470 As Substrate

Site PTM Type Enzyme
S60 Phosphorylation
S68 Phosphorylation
K73 Ubiquitination
Y77 Phosphorylation
K88 Ubiquitination
S94 Phosphorylation
S96 Phosphorylation
K108 Ubiquitination
S139 Phosphorylation
K141 Ubiquitination
T209 Phosphorylation
T214 Phosphorylation
Y235 Phosphorylation
T248 Phosphorylation
S255 Phosphorylation
T258 Phosphorylation
K260 Ubiquitination
Y277 Phosphorylation
Y287 Phosphorylation
Y353 Phosphorylation
S354 Phosphorylation
S468 Phosphorylation
K475 Methylation
S500 Phosphorylation
S502 Phosphorylation O14757 (CHEK1)
S505 Phosphorylation
S508 Phosphorylation
T514 Phosphorylation
S518 Phosphorylation
S519 Phosphorylation
S546 Phosphorylation
S550 Phosphorylation
S553 Phosphorylation
K563 Ubiquitination
K577 Ubiquitination
S582 Phosphorylation
S587 Phosphorylation
T612 Phosphorylation
Y651 Phosphorylation
S659 Phosphorylation
Y661 Phosphorylation

Research Backgrounds

Function:

Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction.

PTMs:

Autophosphorylated on tyrosine residues.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all umbilical cord blood, bone marrow and adult blood cell sub-populations and in several leukemia cell lines. Highly expressed in fetal blood, brain, lung, liver and kidney. Detected at lower levels in adult prostate, testis, ovary, small intestine and colon. Not expressed in adult lung, liver, kidney or brain.

Subunit Structure:

Interacts with the SH3 domain of PLCG1 via its Pro-rich domain.

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family.

Restrictive clause

 

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