Phospho-TBC1D1 (Thr590) Antibody - #AF2422
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| Product: | Phospho-TBC1D1 (Thr590) Antibody |
| Catalog: | AF2422 |
| Description: | Rabbit polyclonal antibody to Phospho-TBC1D1 (Thr590) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 160kDa; |
| Uniprot: | Q60949 |
| RRID: | AB_2819092 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-TBC1D1 (Thr590) Antibody detects endogenous levels of TBC1D1 only when phosphorylated at Thr590.
RRID:
AB_2819092
Cite Format: Affinity Biosciences Cat# AF2422, RRID:AB_2819092.
Cite Format: Affinity Biosciences Cat# AF2422, RRID:AB_2819092.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
KIAA1108; TBC 1; TBC; TBC1 (tre 2/USP6, BUB2, cdc16) domain family, member 1; TBC1; TBC1 domain family member 1; Tbc1d1; TBCD1_HUMAN;
Immunogens
Immunogen:
A synthesized peptide derived from mouse TBC1D1 around the phosphorylation site of Thr590.
PTMs - Q60949 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y113 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| S501 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| S519 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| S559 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| S564 | Phosphorylation | Uniprot | |
| S565 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| T590 | Phosphorylation | P31750 (Akt1) | Uniprot |
| S592 | Phosphorylation | Uniprot | |
| S607 | Phosphorylation | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| S612 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| T624 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| K669 | Methylation | Uniprot | |
| S697 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| S699 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| T739 | Phosphorylation | Uniprot | |
| S747 | Phosphorylation | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| S1028 | Phosphorylation | Uniprot | |
| Y1039 | Phosphorylation | Uniprot | |
| K1179 | Ubiquitination | Uniprot | |
| T1218 | Phosphorylation | Uniprot | |
| S1237 | Phosphorylation | Uniprot | |
| S1241 | Phosphorylation | Uniprot | |
| T1242 | Phosphorylation | Uniprot |
References
1). HWL-088, a new highly potent FFAR1 agonist, improves glucolipid metabolism and acts additively with Metformin in ob/ob diabetic mice. BRITISH JOURNAL OF PHARMACOLOGY, 2020
(PubMed: 31971610)
[IF=7.3]
Application: WB Species: mice Sample: liver
FIGURE 4 Effects of HWL-088 on plasma glucose levels (a) and corresponding AUC0–90min (b) of glucose during an OGTT in fasting ob/ob
mice. Tested agents or vehicle was orally administered to ob/ob mice twice daily for 30 days, and the OGTTs were determined on treatment days
1, 15, and 30. After long-term treatment, hepatic expression of genes involved in gluconeogenesis (c) and glycogen synthesis (d) as well as hepatic
expression of GSK3β (e). Effects of HWL-088 on the phosphorylation of AKT, AS160, and TBC1D1 in L6 myoblasts cells (f). Values are
mean ± SD (n = 6). *P ≤ .05 was analysed using a one-way ANOVA with Tukey's multiple-comparison post hoc test
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