Phospho-SSH3 (Ser37) Antibody - #AF2413

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Product: Phospho-SSH3 (Ser37) Antibody
Catalog: AF2413
Description: Rabbit polyclonal antibody to Phospho-SSH3 (Ser37)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 90kDa; 73kD(Calculated).
Uniprot: Q8TE77
RRID: AB_2845427

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-SSH3 (Ser37) Antibody detects endogenous levels of SSH3 only when phosphorylated at Ser37.
RRID:
AB_2845427
Cite Format: Affinity Biosciences Cat# AF2413, RRID:AB_2845427.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ10928; FLJ20515; hSSH 3L; hSSH-3L; hSSH3L; Protein phosphatase Slingshot homolog 3; Slingshot 3; Slingshot homolog 3; Slingshot3; SSH 3; SSH 3L; SSH-3L; SSH-like protein 3; Ssh3; SSH3_HUMAN; SSH3L;

Immunogens

Immunogen:
Uniprot:

Q8TE77(SSH3_HUMAN) >>Visit HPA database.

Gene(ID):
SSH3(54961)
Sequence:
MALVTVSRSPPGSGASTPVGPWDQAVQRRSRLQRRQSFAVLRGAVLGLQDGGDNDDAAEASSEPTEKAPSEEELHGDQTDFGQGSQSPQKQEEQRQHLHLMVQLLRPQDDIRLAAQLEAPRPPRLRYLLVVSTREGEGLSQDETVLLGVDFPDSSSPSCTLGLVLPLWSDTQVYLDGDGGFSVTSGGQSRIFKPISIQTMWATLQVLHQACEAALGSGLVPGGSALTWASHYQERLNSEQSCLNEWTAMADLESLRPPSAEPGGSSEQEQMEQAIRAELWKVLDVSDLESVTSKEIRQALELRLGLPLQQYRDFIDNQMLLLVAQRDRASRIFPHLYLGSEWNAANLEELQRNRVTHILNMAREIDNFYPERFTYHNVRLWDEESAQLLPHWKETHRFIEAARAQGTHVLVHCKMGVSRSAATVLAYAMKQYECSLEQALRHVQELRPIARPNPGFLRQLQIYQGILTASRQSHVWEQKVGGVSPEEHPAPEVSTPFPPLPPEPEGGGEEKVVGMEESQAAPKEEPGPRPRINLRGVMRSISLLEPSLELESTSETSDMPEVFSSHESSHEEPLQPFPQLARTKGGQQVDRGPQPALKSRQSVVTLQGSAVVANRTQAFQEQEQGQGQGQGEPCISSTPRFRKVVRQASVHDSGEEGEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TE77 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
S9 Phosphorylation
S13 Phosphorylation
S16 Phosphorylation
T17 Phosphorylation
S37 Phosphorylation Q96GD4 (AURKB)
K67 Acetylation
S70 Phosphorylation
T79 Phosphorylation
S85 Phosphorylation
S87 Phosphorylation
K90 Ubiquitination
S259 Phosphorylation
K281 Ubiquitination
S290 Phosphorylation
T292 Phosphorylation
S293 Phosphorylation
K294 Ubiquitination
S385 Phosphorylation
S484 Phosphorylation
K523 Sumoylation
S547 Phosphorylation
K598 Methylation
S602 Phosphorylation
T605 Phosphorylation
S609 Phosphorylation
S637 Phosphorylation
T638 Phosphorylation
S649 Phosphorylation
S653 Phosphorylation

Research Backgrounds

Function:

Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Does not bind to, or colocalize with, filamentous actin.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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