Phospho-SHP1 (Tyr564) Antibody | Affinity Biosciences

WB
IHC
IF/ICC
pElisa

1/4

Western blot analysis of extracts from PC12 cells(LPS 4h treatment), using Phospho-SHP1 (Tyr564) Antibody.

AF2406 at 1/100 staining Mouse liver tissue by IHC-P.

AF2406 staining heat-shock treated Hela cells by IF/ICC.

peptide-ELISA analysis of AF2406.

Product:	Phospho-SHP1 (Tyr564) Antibody
Catalog:	AF2406
Description:	Rabbit polyclonal antibody to Phospho-SHP1 (Tyr564)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog
Mol.Wt.:	68kDa; 68kD(Calculated).
Uniprot:	P29350
RRID:	AB_2845420

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(82%), Zebrafish(100%), Bovine(80%), Horse(91%), Sheep(82%), Dog(82%)

Clonality:

Polyclonal

Specificity:

Phospho-SHP1 (Tyr564) Antibody detects endogenous levels of SHP1 only when phosphorylated at Tyr564.

RRID:

AB_2845420
Cite Format: Affinity Biosciences Cat# AF2406, RRID:AB_2845420.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

70Z-SHP; EC 3.1.3.48; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP1; Protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP 1; Protein-tyrosine phosphatase SHP-1; PTN6_HUMAN; PTP 1C; PTP-1C; PTP1C; Ptpn6; SH PTP 1; SH PTP1; SH-PTP1; SHP 1; SHP 1L; SHP1; SHP1L; tyrosine protein phosphatase non receptor type 6; Tyrosine-protein phosphatase non-receptor type 6;

Immunogens

Immunogen:

Uniprot:

P29350(PTN6_HUMAN) >>Visit HPA database.

Gene(ID):

PTPN6(5777)

Expression:

P29350 PTN6_HUMAN:

Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.

Sequence:

P29350 PTN6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVRWFHRDLSGLDAETLLKGRGVHGSFLARPSRKNQGDFSLSVRVGDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGVLQDRDGTIIHLKYPLNCSDPTSERWYHGHMSGGQAETLLQAKGEPWTFLVRESLSQPGDFVLSVLSDQPKAGPGSPLRVTHIKVMCEGGRYTVGGLETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Zebrafish

100

Horse

Pig

Sheep

Dog

Bovine

Xenopus

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29350 As Substrate

P29350

Site	PTM Type	Enzyme	Source
S10	Phosphorylation		Uniprot
K19	Ubiquitination		Uniprot
S26	Phosphorylation		Uniprot
K34	Ubiquitination		Uniprot
S57	Phosphorylation		Uniprot
Y61	Phosphorylation		Uniprot
Y64	Phosphorylation		Uniprot
K97	Ubiquitination		Uniprot
Y98	Phosphorylation		Uniprot
Y111	Phosphorylation		Uniprot
S140	Phosphorylation		Uniprot
K155	Ubiquitination		Uniprot
K168	Ubiquitination		Uniprot
Y176	Phosphorylation		Uniprot
T177	Phosphorylation		Uniprot
S186	Phosphorylation		Uniprot
T188	Phosphorylation		Uniprot
K195	Ubiquitination		Uniprot
K196	Ubiquitination		Uniprot
Y208	Phosphorylation		Uniprot
Y213	Phosphorylation		Uniprot
Y214	Phosphorylation		Uniprot
K232	Ubiquitination		Uniprot
K241	Ubiquitination		Uniprot
S250	Phosphorylation		Uniprot
K253	Ubiquitination		Uniprot
K257	Ubiquitination		Uniprot
K271	Ubiquitination		Uniprot
Y276	Phosphorylation		Uniprot
K277	Acetylation		Uniprot
K277	Ubiquitination		Uniprot
S285	Phosphorylation		Uniprot
R286	Methylation		Uniprot
S299	Phosphorylation		Uniprot
Y301	Phosphorylation		Uniprot
Y306	Phosphorylation		Uniprot
K308	Ubiquitination		Uniprot
K360	Ubiquitination		Uniprot
Y364	Phosphorylation		Uniprot
Y374	Phosphorylation		Uniprot
Y377	Phosphorylation		Uniprot
S378	Phosphorylation		Uniprot
K391	Ubiquitination		Uniprot
T394	Phosphorylation		Uniprot
S398	Phosphorylation		Uniprot
C453	S-Nitrosylation		Uniprot
K486	Ubiquitination		Uniprot
S496	Phosphorylation		Uniprot
Y509	Phosphorylation		Uniprot
K521	Ubiquitination		Uniprot
K522	Ubiquitination		Uniprot
S528	Phosphorylation		Uniprot
K530	Ubiquitination		Uniprot
S534	Phosphorylation		Uniprot
Y536	Phosphorylation	P06213 (INSR) , P00519 (ABL1) , P12931 (SRC) , A0A173G4P4 (Abl fusion) , P06239 (LCK) , P07948 (LYN)	Uniprot
T540	Phosphorylation		Uniprot
Y541	Phosphorylation		Uniprot
K546	Ubiquitination		Uniprot
S553	Phosphorylation		Uniprot
T555	Phosphorylation		Uniprot
S556	Phosphorylation		Uniprot
S557	Phosphorylation		Uniprot
K560	Ubiquitination		Uniprot
Y564	Phosphorylation	A0A173G4P4 (Abl fusion) , P00519 (ABL1) , P07948 (LYN) , P06239 (LCK) , P12931 (SRC)	Uniprot
K570	Ubiquitination		Uniprot
S582	Phosphorylation		Uniprot
S588	Phosphorylation		Uniprot
S591	Phosphorylation	P17252 (PRKCA) , P24941 (CDK2)	Uniprot

Research Backgrounds

P29350_PTN6_HUMAN

Function:

Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.

PTMs:

Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation (By similarity). Phosphorylation at Tyr-564 enhances phosphatase activity.

Subcellular Location:

Cytoplasm. Nucleus.
Note: In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1.

Tissue Specificity:

Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.

Subunit Structure:

Monomer. Interacts with MTUS1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated) (By similarity). Interacts with KIT (By similarity). Interacts with SIRPA/PTPNS1. Interacts with LILRB1 and LILRB2. Interacts with FCRL2 and FCRL4. Interacts with FCRL3 and FCRL6 (tyrosine phosphorylated form). Interacts with CD84. Interacts with CD300LF. Interacts with CDK2. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1. Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity). Interacts with MPIG6B (via ITIM motif). Interacts with moesin/MSN.

Family&Domains:

The N-terminal SH2 domain functions as an auto-inhibitory domain, blocking the catalytic domain in the ligand-free close conformation.

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)

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Phospho-SHP1 (Tyr564) Antibody - #AF2406