Product: Phospho-RSK2 (Ser227) Antibody
Catalog: AF2399
Description: Rabbit polyclonal antibody to Phospho-RSK2 (Ser227)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 90kDa; 84kD(Calculated).
Uniprot: P51812
RRID: AB_2845413

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-RSK2 (Ser227) Antibody detects endogenous levels of RSK2 only when phosphorylated at Ser227.
RRID:
AB_2845413
Cite Format: Affinity Biosciences Cat# AF2399, RRID:AB_2845413.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

90 kDa ribosomal protein S6 kinase 3; CLS; HU 3; HU2; HU3; Insulin stimulated protein kinase 1; Insulin-stimulated protein kinase 1; ISPK-1; ISPK1; KS6A3_HUMAN; MAP kinase activated protein kinase 1b; MAP kinase-activated protein kinase 1b; MAPK activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK 1b; MAPKAPK-1b; MAPKAPK1B; Mental retardation, X linked 19; MRX19; OTTHUMP00000023036; p90 RSK2; p90 RSK3; p90-RSK 3; p90RSK3; pp90RSK2; Ribosomal protein S6 kinase 90kDa polypeptide 3; Ribosomal protein S6 kinase alpha 3; Ribosomal protein S6 kinase alpha-3; Ribosomal protein s6 kinase ii alpha 2; Ribosomal S6 kinase 2; Rps6ka3; RSK; RSK-2; RSK2; S6 kinase 2; S6K alpha3; S6K-alpha-3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P51812 KS6A3_HUMAN:

Expressed in many tissues, highest levels in skeletal muscle.

Sequence:
MPLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEINPQTEEVSIKEIAITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVKKISGSDARQLYAMKVLKKATLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKESIDHEKKAYSFCGTVEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGTLPFQGKDRKETMTMILKAKLGMPQFLSPEAQSLLRMLFKRNPANRLGAGPDGVEEIKRHSFFSTIDWNKLYRREIHPPFKPATGRPEDTFYFDPEFTAKTPKDSPGIPPSANAHQLFRGFSFVAITSDDESQAMQTVGVHSIVQQLHRNSIQFTDGYEVKEDIGVGSYSVCKRCIHKATNMEFAVKIIDKSKRDPTEEIEILLRYGQHPNIITLKDVYDDGKYVYVVTELMKGGELLDKILRQKFFSEREASAVLFTITKTVEYLHAQGVVHRDLKPSNILYVDESGNPESIRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGVLLYTMLTGYTPFANGPDDTPEEILARIGSGKFSLSGGYWNSVSDTAKDLVSKMLHVDPHQRLTAALVLRHPWIVHWDQLPQYQLNRQDAPHLVKGAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKITSTAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51812 As Substrate

Site PTM Type Enzyme
S17 Phosphorylation
S19 Phosphorylation
K57 Ubiquitination
K62 Ubiquitination
K72 Ubiquitination
S78 Phosphorylation
K81 Acetylation
K100 Acetylation
K103 Acetylation
Y144 Phosphorylation
K195 Ubiquitination
S215 Phosphorylation
K216 Ubiquitination
Y226 Phosphorylation
S227 Phosphorylation P51812 (RPS6KA3) , P27361 (MAPK3) , O15530 (PDPK1)
T231 Phosphorylation P51812 (RPS6KA3)
Y234 Phosphorylation
K274 Ubiquitination
T278 Phosphorylation
K322 Ubiquitination
K345 Ubiquitination
Y356 Phosphorylation
T362 Phosphorylation
K364 Ubiquitination
T365 Phosphorylation P51812 (RPS6KA3)
S369 Phosphorylation P51812 (RPS6KA3)
S375 Phosphorylation
S386 Phosphorylation P28482 (MAPK1) , P51812 (RPS6KA3) , P49137 (MAPKAPK2) , O15530 (PDPK1)
T391 Phosphorylation
S392 Phosphorylation
S396 Phosphorylation
S415 Phosphorylation
Y433 Phosphorylation
K437 Ubiquitination
Y470 Phosphorylation P12931 (SRC) , P06241 (FYN)
T478 Phosphorylation
K480 Ubiquitination
Y483 Phosphorylation P06241 (FYN) , P12931 (SRC)
Y488 Phosphorylation P12931 (SRC) , P06241 (FYN) , P28482 (MAPK1) , P22607 (FGFR3)
K504 Ubiquitination
K509 Ubiquitination
S517 Phosphorylation
Y529 Phosphorylation P12931 (SRC) , P28482 (MAPK1) , P22607 (FGFR3) , P06241 (FYN)
K541 Ubiquitination
S556 Phosphorylation
K566 Ubiquitination
T573 Phosphorylation
T577 Phosphorylation P27361 (MAPK3)
Y580 Phosphorylation P06241 (FYN) , P12931 (SRC)
T581 Phosphorylation P51812 (RPS6KA3)
K591 Ubiquitination
T625 Phosphorylation
Y644 Phosphorylation P12931 (SRC) , P06241 (FYN)
K658 Ubiquitination
Y688 Phosphorylation
T706 Phosphorylation
Y707 Phosphorylation P06241 (FYN) , P35916 (FLT4) , P11362 (FGFR1) , P22607 (FGFR3) , P12931 (SRC)
S708 Phosphorylation
S715 Phosphorylation P51812 (RPS6KA3)
R723 Methylation
S737 Phosphorylation

PTMs - P51812 As Enzyme

Substrate Site Source
O14649 (KCNK3) S393 Uniprot
O60825 (PFKFB2) S466 Uniprot
P03372 (ESR1) S167 Uniprot
P04637 (TP53) S15 Uniprot
P04792 (HSPB1) S78 Uniprot
P04792 (HSPB1) S82 Uniprot
P05114 (HMGN1) S7 Uniprot
P05114 (HMGN1) S21 Uniprot
P05114 (HMGN1) S25 Uniprot
P05198 (EIF2S1) S52 Uniprot
P05204 (HMGN2) S25 Uniprot
P05204 (HMGN2) S29 Uniprot
P05783 (KRT18) S53 Uniprot
P07101 (TH) S40 Uniprot
P07101-4 (TH) S44 Uniprot
P07101-2 (TH) S67 Uniprot
P07101 (TH) S71 Uniprot
P10636-6 (MAPT) T154 Uniprot
P10636-8 (MAPT) T212 Uniprot
P11362 (FGFR1) S789 Uniprot
P13796 (LCP1) S5 Uniprot
P16220 (CREB1) S133 Uniprot
P17676 (CEBPB) S321 Uniprot
P18846 (ATF1) S63 Uniprot
P18848 (ATF4) S245 Uniprot
P19525 (EIF2AK2) T451 Uniprot
P22736-1 (NR4A1) S351 Uniprot
P23588 (EIF4B) S422 Uniprot
P25963 (NFKBIA) S32 Uniprot
P29317 (EPHA2) S897 Uniprot
P33778 (HIST1H2BB) S33 Uniprot
P40763 (STAT3) S727 Uniprot
P46527 (CDKN1B) T198 Uniprot
P48764 (SLC9A3) S663 Uniprot
P49815 (TSC2) S1798 Uniprot
P49840 (GSK3A) S21 Uniprot
P49841 (GSK3B) S9 Uniprot
P51812 (RPS6KA3) S227 Uniprot
P51812 (RPS6KA3) T231 Uniprot
P51812 (RPS6KA3) T365 Uniprot
P51812 (RPS6KA3) S369 Uniprot
P51812 (RPS6KA3) S386 Uniprot
P51812 (RPS6KA3) T581 Uniprot
P51812 (RPS6KA3) S715 Uniprot
P53355 (DAPK1) S289 Uniprot
P62753 (RPS6) S235 Uniprot
P62753 (RPS6) S236 Uniprot
P67809 (YBX1) S102 Uniprot
P68431 (HIST1H3J) S11 Uniprot
P84243 (H3F3B) S11 Uniprot
Q04206 (RELA) S536 Uniprot
Q06413 (MEF2C) S192 Uniprot
Q14790 (CASP8) T263 Uniprot
Q14934 (NFATC4) S281 Uniprot
Q14934 (NFATC4) S285 Uniprot
Q14934 (NFATC4) S289 Uniprot
Q14934 (NFATC4) S344 Uniprot
Q14934 (NFATC4) S676 Uniprot
Q15831-1 (STK11) S428 Uniprot
Q16695 (HIST3H3) S11 Uniprot
Q16821 (PPP1R3A) S46 Uniprot
Q16821 (PPP1R3A) S65 Uniprot
Q8IX03 (WWC1) T929 Uniprot
Q8IX03 (WWC1) S947 Uniprot
Q8N122 (RPTOR) S719 Uniprot
Q8N122 (RPTOR) S721 Uniprot
Q8N122 (RPTOR) S722 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S99 Uniprot
Q96RK0 (CIC) S173 Uniprot
Q99683 (MAP3K5) S83 Uniprot
Q99683 (MAP3K5) T1109 Uniprot
Q99683 (MAP3K5) T1326 Uniprot
Q9BSI4 (TINF2) S295 Uniprot
Q9BSI4 (TINF2) S330 Uniprot
Q9H6Z4 (RANBP3) S126 Uniprot
Q9NYV6 (RRN3) S649 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Negatively regulates EGF-induced MAPK1/3 phosphorylation via phosphorylation of SOS1. Phosphorylates SOS1 at 'Ser-1134' and 'Ser-1161' that create YWHAB and YWHAE binding sites and which contribute to the negative regulation of MAPK1/3 phosphorylation (By similarity). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration.

PTMs:

Activated by phosphorylation at Ser-227 by PDPK1. Autophosphorylated on Ser-386, as part of the activation process. May be phosphorylated at Thr-365 and Ser-369 by MAPK1/ERK2 and MAPK3/ERK1. Can also be activated via phosphorylation at Ser-386 by MAPKAPK2.

N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

Subcellular Location:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in many tissues, highest levels in skeletal muscle.

Subunit Structure:

Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). Interacts with NFATC4, ETV1/ER81 and FGFR1.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

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