Phospho-REPS1 (Ser709) Antibody - #AF2396

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Product: Phospho-REPS1 (Ser709) Antibody
Catalog: AF2396
Description: Rabbit polyclonal antibody to Phospho-REPS1 (Ser709)
Application: WB IHC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 80~110kD; 87kD(Calculated).
Uniprot: Q96D71
RRID: AB_2845410

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-REPS1 (Ser709) Antibody detects endogenous levels of REPS1 only when phosphorylated at Ser709.
RRID:
AB_2845410
Cite Format: Affinity Biosciences Cat# AF2396, RRID:AB_2845410.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

RALBP1; RalBP1 associated Eps domain containing protein 1; RalBP1 interacting protein 1; RalBP1-associated Eps domain-containing protein 1; RalBP1-interacting protein 1; REPS1; REPS1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q96D71(REPS1_HUMAN) >>Visit HPA database.

Gene(ID):
REPS1(85021)
Expression:
Q96D71 REPS1_HUMAN:

Widely expressed with highest levels in heart and testis.

Sequence:
MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQESRHAASYSSDSENQGSYSGVIPPPPGRGQVKKGSVSHDTVQPRTSADAQEPASPVVSPQQSPPTSPHTWRKHSRHPSGGNSERPLAGPGPFWSPFGEAQSGSSAGDAVWSGHSPPPPQENWVSFADTPPTSTLLTMHPASVQDQTTVRTVASATTAIEIRRQSSSYDDPWKITDEQRQYYVNQFKTIQPDLNGFIPGSAAKEFFTKSKLPILELSHIWELSDFDKDGALTLDEFCAAFHLVVARKNGYDLPEKLPESLMPKLIDLEDSADVGDQPGEVGYSGSPAEAPPSKSPSMPSLNQTWPELNQSSEQWETFSERSSSSQTLTQFDSNIAPADPDTAIVHPVPIRMTPSKIHMQEMELKRTGSDHTNPTSPLLVKPSDLLEENKINSSVKFASGNTVADGYSSSDSFTSDPEQIGSNVTRQRSHSGTSPDNTAPPPPPPRPQPSHSRSSSLDMNRTFTVTTGQQQAGVVAHPPAVPPRPQPSQAPGPAVHRPVDADGLITHTSTSPQQIPEQPNFADFSQFEVFAASNVNDEQDDEAEKHPEVLPAEKASDPASSLRVAKTDSKTEEKTAASAPANVSKGTTPLAPPPKPVRRRLKSEDELRPEVDEHTQKTGVLAAVLASQPSIPRSVGKDKKAIQASIRRNKETNTVLARLNSELQQQLKDVLEERISLEVQLEQLRPFSHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96D71 As Substrate

Site PTM Type Enzyme
K27 Ubiquitination
S81 Phosphorylation
S104 Phosphorylation
S110 Phosphorylation
S115 Phosphorylation
Y116 Phosphorylation
S117 Phosphorylation
S118 Phosphorylation
S120 Phosphorylation
S125 Phosphorylation
Y126 Phosphorylation
S127 Phosphorylation
S143 Phosphorylation
S145 Phosphorylation
T148 Phosphorylation
T153 Phosphorylation
S162 Phosphorylation
S166 Phosphorylation
S170 Phosphorylation
T173 Phosphorylation
S174 Phosphorylation
T177 Phosphorylation
S186 Phosphorylation
S190 Phosphorylation
S261 Phosphorylation
T263 Phosphorylation
T264 Phosphorylation
S272 Phosphorylation
S273 Phosphorylation
S274 Phosphorylation
Y275 Phosphorylation
S307 Phosphorylation
S377 Phosphorylation
Y389 Phosphorylation
S390 Phosphorylation
S392 Phosphorylation
S399 Phosphorylation
S401 Phosphorylation
S429 Phosphorylation
S430 Phosphorylation
S431 Phosphorylation
T459 Phosphorylation
S461 Phosphorylation
K462 Ubiquitination
K471 Methylation
T473 Phosphorylation
S475 Phosphorylation
T478 Phosphorylation
T481 Phosphorylation
S482 Phosphorylation
S489 Phosphorylation
S500 Phosphorylation
S515 Phosphorylation
S518 Phosphorylation
S535 Phosphorylation
S537 Phosphorylation
T539 Phosphorylation
S540 Phosphorylation
T544 Phosphorylation
S556 Phosphorylation
S558 Phosphorylation
S560 Phosphorylation
S561 Phosphorylation
S562 Phosphorylation
S667 Phosphorylation
T694 Phosphorylation
K708 Ubiquitination
S709 Phosphorylation
T721 Phosphorylation
S736 Phosphorylation
S740 Phosphorylation
S751 Phosphorylation
T758 Phosphorylation
S767 Phosphorylation
S794 Phosphorylation

Research Backgrounds

Function:

May coordinate the cellular actions of activated EGF receptors and Ral-GTPases.

PTMs:

EGF stimulates phosphorylation on Tyr-residues.

Subcellular Location:

Membrane>Clathrin-coated pit.
Note: Colocalize with ITSN1 at the plasma membrane in structures that are most probably clathrin-coated pits.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with highest levels in heart and testis.

Subunit Structure:

Homodimer (Potential). Interacts with RAB11FIP2. Interacts with RALBP1, CRK and GRB2. Binding to RALBP1 does not affect its Ral-binding activity. Forms a complex with the SH3 domains of CRK and GRB2 which may link it to an EGF-responsive tyrosine kinase (By similarity). Interacts with AMPH, ITSN1 (via SH3 domains) and SGIP1; may be involved in clathrin-mediated endocytosis.

Restrictive clause

 

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