Product: Phospho-PTP alpha (Tyr798) Antibody
Catalog: AF2392
Description: Rabbit polyclonal antibody to Phospho-PTP alpha (Tyr798)
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 145kDa; 91kD(Calculated).
Uniprot: P18433
RRID: AB_2845406

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-PTP alpha (Tyr798) Antibody detects endogenous levels of PTP alpha only when phosphorylated at Tyr798.
RRID:
AB_2845406
Cite Format: Affinity Biosciences Cat# AF2392, RRID:AB_2845406.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HEPTP; HLPR; HPTP alpha; HPTPA; HPTPalpha; Leukocyte common antigen related peptide; LRP; Protein tyrosine phosphatase alpha; Protein tyrosine phosphatase receptor type A; Protein tyrosine phosphatase receptor type alpha polypeptide; Protein tyrosine phosphate; Protein-tyrosine phosphatase alpha; PTPA; PTPalpha; PTPase alpha; PTPLCA related phosphatase; Ptpra; PTPRA_HUMAN; PTPRL 2; PTPRL2; PTRA; R PTP alpha; R-PTP-alpha; Receptor type tyrosine protein phosphatase alpha; Receptor-type tyrosine-protein phosphatase alpha; RPTPA; Tyrosine phosphatase alpha;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MDSWFILVLLGSGLICVSANNATTVAPSVGITRLINSSTAEPVKEEAKTSNPTSSLTSLSVAPTFSPNITLGPTYLTTVNSSDSDNGTTRTASTNSIGITISPNGTWLPDNQFTDARTEPWEGNSSTAATTPETFPPSGNSDSKDRRDETPIIAVMVALSSLLVIVFIIIVLYMLRFKKYKQAGSHSNSFRLSNGRTEDVEPQSVPLLARSPSTNRKYPPLPVDKLEEEINRRMADDNKLFREEFNALPACPIQATCEAASKEENKEKNRYVNILPYDHSRVHLTPVEGVPDSDYINASFINGYQEKNKFIAAQGPKEETVNDFWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNIRVSVEDVTVLVDYTVRKFCIQQVGDMTNRKPQRLITQFHFTSWPDFGVPFTPIGMLKFLKKVKACNPQYAGAIVVHCSAGVGRTGTFVVIDAMLDMMHTERKVDVYGFVSRIRAQRCQMVQTDMQYVFIYQALLEHYLYGDTELEVTSLETHLQKIYNKIPGTSNNGLEEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSYIASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERGQEKCAQYWPSDGLVSYGDITVELKKEEECESYTVRDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGMISIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18433 As Substrate

Site PTM Type Enzyme
S18 Phosphorylation
S187 Phosphorylation
S189 Phosphorylation Q05655 (PRKCD)
S204 Phosphorylation Q9UQM7 (CAMK2A) , Q05655 (PRKCD)
S211 Phosphorylation
S213 Phosphorylation Q05655 (PRKCD)
T214 Phosphorylation
Y218 Phosphorylation
K225 Ubiquitination
Y271 Phosphorylation
S293 Phosphorylation
K307 Ubiquitination
S407 Phosphorylation
Y522 Phosphorylation
K524 Ubiquitination
S542 Phosphorylation
T552 Phosphorylation
K560 Ubiquitination
Y588 Phosphorylation
K765 Acetylation
S766 Phosphorylation
Y791 Phosphorylation
S796 Phosphorylation
Y798 Phosphorylation P12931 (SRC)

Research Backgrounds

Function:

Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation (By similarity). Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42 (By similarity).

PTMs:

Integrin binding to extracellular matrix induces phosphorylation at Tyr-798 which induces PTPRA localization and recruitment of BCAR3, BCAR1 and CRK to focal adhesions.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell junction>Focal adhesion.
Note: Localizes to focal adhesion sites following integrin engagement.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Part of a complex comprised of PTPRA, BCAR1, BCAR3 (via SH2 domain), and SRC. Within the complex, interacts (when phosphorylated on Tyr-798) with BCAR3 (via SH2 domain). Interacts with GRB2.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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