Phospho-p40 phox (Thr154) Antibody - #AF2376

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Product: Phospho-p40 phox (Thr154) Antibody
Catalog: AF2376
Description: Rabbit polyclonal antibody to Phospho-p40 phox (Thr154)
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 40kDa; 39kD(Calculated).
Uniprot: Q15080
RRID: AB_2845390

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(83%), Horse(82%), Rabbit(100%), Dog(91%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Phospho-p40 phox (Thr154) Antibody detects endogenous levels of p40 phox only when phosphorylated at Thr154.
RRID:
AB_2845390
Cite Format: Affinity Biosciences Cat# AF2376, RRID:AB_2845390.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CGD3; MGC3810; NCF 4; NCF; NCF-4; Ncf4; NCF4_HUMAN; Neutrophil cytosol factor 4; Neutrophil cytosolic factor 4; Neutrophil NADPH oxidase factor 4; p40-phox; p40phox; SH3 and PX domain-containing protein 4; SH3PXD4;

Immunogens

Immunogen:
Uniprot:

Q15080(NCF4_HUMAN) >>Visit HPA database.

Gene(ID):
NCF4(4689)
Expression:
Q15080 NCF4_HUMAN:

Expression is restricted to hematopoietic cells.

Sequence:
MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIAEMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Dog
91
Xenopus
90
Zebrafish
83
Horse
82
Chicken
67
Bovine
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15080 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
S23 Phosphorylation
T47 Phosphorylation
S51 Phosphorylation
Y53 Phosphorylation
T154 Phosphorylation Q05655 (PRKCD) , P17252 (PRKCA)
S161 Phosphorylation
S166 Phosphorylation
K228 Ubiquitination
Y243 Phosphorylation
Y245 Phosphorylation
S299 Phosphorylation
S315 Phosphorylation P17252 (PRKCA)

Research Backgrounds

Function:

Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.

Subcellular Location:

Cytoplasm>Cytosol. Endosome membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expression is restricted to hematopoietic cells.

Subunit Structure:

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. NCF4 interacts primarily with NCF2 to form a complex with NCF1.

Family&Domains:

The PB1 domain mediates the association with NCF2/p67-PHOX.

The PX domain mediates interaction with membranes enriched in phosphatidylnositol 3-phosphate.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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For Research Use Only.
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