Phospho-p130 Cas (Tyr249) Antibody - #AF2375

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Product: Phospho-p130 Cas (Tyr249) Antibody
Catalog: AF2375
Description: Rabbit polyclonal antibody to Phospho-p130 Cas (Tyr249)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 130kDa; 93kD(Calculated).
Uniprot: P56945
RRID: AB_2845389

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Chicken(83%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
Phospho-p130 Cas (Tyr249) Antibody detects endogenous levels of p130 Cas only when phosphorylated at Tyr249.
RRID:
AB_2845389
Cite Format: Affinity Biosciences Cat# AF2375, RRID:AB_2845389.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BCAR 1; Bcar1; BCAR1_HUMAN; Breast cancer anti estrogen resistance 1; Breast cancer anti estrogen resistance 1 protein; Breast cancer anti-estrogen resistance protein 1; CAS; Cas scaffolding protein family member 1; CAS1; Cass1; Crk associated substrate; Crk associated substrate p130Cas; CRK-associated substrate; CRKAS; FLJ12176; FLJ45059; p130cas;

Immunogens

Immunogen:
Uniprot:

P56945(BCAR1_HUMAN) >>Visit HPA database.

Gene(ID):
BCAR1(9564)
Expression:
P56945 BCAR1_HUMAN:

Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.

Sequence:
MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGPGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPSPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPAEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVERVKELGHSTQQFRRVLGQLAAA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Horse
92
Zebrafish
90
Xenopus
89
Chicken
83
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56945 As Substrate

Site PTM Type Enzyme
Phosphorylation
Y12 Phosphorylation P51813 (BMX)
S18 Phosphorylation
Y67 Phosphorylation
S113 Phosphorylation
Y115 Phosphorylation
Y128 Phosphorylation P11362 (FGFR1) , P12931 (SRC)
S134 Phosphorylation
S139 Phosphorylation
K143 Acetylation
Y165 Phosphorylation Q13882 (PTK6) , Q07912 (TNK2)
Y179 Phosphorylation
S184 Phosphorylation
Y192 Phosphorylation
S202 Phosphorylation
Y222 Phosphorylation
Y224 Phosphorylation Q13882 (PTK6)
Y234 Phosphorylation Q13882 (PTK6)
Y249 Phosphorylation Q13882 (PTK6) , P11362 (FGFR1) , P12931 (SRC) , Q07912 (TNK2)
S260 Phosphorylation
Y262 Phosphorylation Q13882 (PTK6)
Y267 Phosphorylation Q13882 (PTK6)
T269 Phosphorylation
Y287 Phosphorylation Q13882 (PTK6)
S292 Phosphorylation
S300 Phosphorylation
Y306 Phosphorylation P11362 (FGFR1) , Q13882 (PTK6)
S311 Phosphorylation
S313 Phosphorylation
T326 Phosphorylation
Y327 Phosphorylation P11362 (FGFR1) , Q13882 (PTK6)
S355 Phosphorylation
Y362 Phosphorylation Q13882 (PTK6)
Y372 Phosphorylation
T385 Phosphorylation
Y387 Phosphorylation Q13882 (PTK6)
S407 Phosphorylation
Y410 Phosphorylation Q05397 (PTK2) , P11362 (FGFR1) , Q07912 (TNK2)
S428 Phosphorylation
S430 Phosphorylation
S431 Phosphorylation
T432 Phosphorylation
S434 Phosphorylation
T435 Phosphorylation
S437 Phosphorylation
S438 Phosphorylation
S440 Phosphorylation
S602 Phosphorylation
S639 Phosphorylation
S648 Phosphorylation
Y653 Phosphorylation P12931 (SRC)
Y664 Phosphorylation Q13882 (PTK6) , P12931 (SRC)
Y666 Phosphorylation P12931 (SRC)
K693 Ubiquitination
S694 Phosphorylation
K702 Ubiquitination
Y815 Phosphorylation
K831 Ubiquitination
K851 Ubiquitination

Research Backgrounds

Function:

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

PTMs:

PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.

Dephosphorylated by PTPN14 at Tyr-128.

Subcellular Location:

Cell junction>Focal adhesion. Cytoplasm.
Note: Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.

Subunit Structure:

Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9. Interacts with BCAR3, the interaction regulates adhesion-dependent serine phosphorylation. Interacts with NPHP1 and SH2D3C. Interacts with activated CSPG4. Interacts with BMX, INPPL1/SHIP2 and PEAK1. Part of a collagen-stimulated complex involved in cell migration made of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with TNK2 via SH3 domains. Interacts with PTK2B/PYK2.

Family&Domains:

Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9 (By similarity).

A serine-rich region promotes activation of the serum response element (SRE).

The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.

Belongs to the CAS family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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