Phospho-Myt1 (Ser83) Antibody | Affinity Biosciences

pElisa

1/1

Phospho-Myt1 (Ser83) Antibody - peptide-ELISA analysis of AF2369.

Product:	Phospho-Myt1 (Ser83) Antibody
Catalog:	AF2369
Description:	Rabbit polyclonal antibody to Phospho-Myt1 (Ser83)
Application:	WB IHC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	70kDa; 55kD(Calculated).
Uniprot:	Q99640
RRID:	AB_2845383

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(92%), Bovine(80%), Horse(92%), Rabbit(83%), Dog(92%)

Clonality:

Polyclonal

Specificity:

Phospho-Myt1 (Ser83) Antibody detects endogenous levels of Myt1 only when phosphorylated at Ser83.

RRID:

AB_2845383
Cite Format: Affinity Biosciences Cat# AF2369, RRID:AB_2845383.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

cdc2 inhibitory kinase; DKFZp547K1610; FLJ20093; Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; PPP1R126; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; Protein phosphatase 1 regulatory subunit 126;

Immunogens

Immunogen:

A synthesized peptide derived from human Myt1 around the phosphorylation site of Ser83.

Uniprot:

Q99640(PMYT1_HUMAN) >>Visit HPA database.

Gene(ID):

PKMYT1(9088)

Sequence:

Q99640 PMYT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLERPPALAMPMPTEGTPPPLSGTPIPVPAYFRHAEPGFSLKRPRGLSRSLPPPPPAKGSIPISRLFPPRTPGWHQLQPRRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRSMSPFRGPKDRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQVWGYLRDTLLALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELLQGSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRATAEALLALPVLRQPRAWGVLWCMAAEALSRGWALWQALLALLCWLWHGLAHPASWLQPLGPPATPPGSPPCSLLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLSLFEDTLDPT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Rabbit

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q99640_PMYT1_HUMAN

Function:

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

PTMs:

Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein.

Family&Domains:

The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-Myt1 (Ser83) Antibody - #AF2369