Phospho-Myt1 (Ser83) Antibody | Affinity Biosciences

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Product:	Phospho-Myt1 (Ser83) Antibody
Catalog:	AF2369
Description:	Rabbit polyclonal antibody to Phospho-Myt1 (Ser83)
Application:	WB IHC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	70kDa; 55kD(Calculated).
Uniprot:	Q99640
RRID:	AB_2845383

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(92%), Bovine(80%), Horse(92%), Rabbit(83%), Dog(92%)

Clonality:

Polyclonal

Specificity:

Phospho-Myt1 (Ser83) Antibody detects endogenous levels of Myt1 only when phosphorylated at Ser83.

RRID:

AB_2845383
Cite Format: Affinity Biosciences Cat# AF2369, RRID:AB_2845383.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

cdc2 inhibitory kinase; DKFZp547K1610; FLJ20093; Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; PPP1R126; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; Protein phosphatase 1 regulatory subunit 126;

Immunogens

Immunogen:

Uniprot:

Q99640(PMYT1_HUMAN) >>Visit HPA database.

Gene(ID):

PKMYT1(9088)

Sequence:

Q99640 PMYT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLERPPALAMPMPTEGTPPPLSGTPIPVPAYFRHAEPGFSLKRPRGLSRSLPPPPPAKGSIPISRLFPPRTPGWHQLQPRRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRSMSPFRGPKDRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQVWGYLRDTLLALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELLQGSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRATAEALLALPVLRQPRAWGVLWCMAAEALSRGWALWQALLALLCWLWHGLAHPASWLQPLGPPATPPGSPPCSLLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLSLFEDTLDPT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Rabbit

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99640 As Substrate

Q99640

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
M1	Acetylation		Uniprot
T17	Phosphorylation		Uniprot
T24	Phosphorylation		Uniprot
S40	Phosphorylation		Uniprot
K42	Methylation		Uniprot
K42	Ubiquitination		Uniprot
S48	Phosphorylation		Uniprot
S50	Phosphorylation		Uniprot
K58	Ubiquitination		Uniprot
S60	Phosphorylation		Uniprot
S64	Phosphorylation		Uniprot
T71	Phosphorylation		Uniprot
S83	Phosphorylation	P31749 (AKT1) , P45983 (MAPK8)	Uniprot
S89	Phosphorylation		Uniprot
S94	Phosphorylation		Uniprot
Y97	Phosphorylation		Uniprot
S109	Phosphorylation		Uniprot
S120	Phosphorylation		Uniprot
K126	Methylation		Uniprot
K126	Ubiquitination		Uniprot
K139	Ubiquitination		Uniprot
S141	Phosphorylation		Uniprot
S143	Phosphorylation		Uniprot
K149	Ubiquitination		Uniprot
K154	Ubiquitination		Uniprot
S160	Phosphorylation		Uniprot
K163	Ubiquitination		Uniprot
K345	Ubiquitination		Uniprot
T412	Phosphorylation		Uniprot
S416	Phosphorylation		Uniprot
S426	Phosphorylation	P53350 (PLK1)	Uniprot
S441	Phosphorylation		Uniprot
T453	Phosphorylation		Uniprot
S454	Phosphorylation		Uniprot
T455	Phosphorylation		Uniprot
S458	Phosphorylation		Uniprot
T461	Phosphorylation		Uniprot
S469	Phosphorylation		Uniprot
S473	Phosphorylation		Uniprot
S479	Phosphorylation		Uniprot
S482	Phosphorylation		Uniprot
T495	Phosphorylation	P53350 (PLK1)	Uniprot

PTMs - Q99640 As Enzyme

Q99640

Substrate	Site	Source
P06493 (CDK1)	T14	Uniprot
P06493 (CDK1)	Y15	Uniprot

Research Backgrounds

Q99640_PMYT1_HUMAN

Function:

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

PTMs:

Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein.

Subunit Structure:

Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1.

Family&Domains:

The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

Restrictive clause

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For Research Use Only.
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Phospho-Myt1 (Ser83) Antibody - #AF2369