Phospho-Cytokeratin 20 (Ser13) Antibody - #AF2356

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Product: Phospho-Cytokeratin 20 (Ser13) Antibody
Catalog: AF2356
Description: Rabbit polyclonal antibody to Phospho-Cytokeratin 20 (Ser13)
Application: WB IHC
Reactivity: Human
Prediction: Bovine, Sheep, Dog
Mol.Wt.: 48kDa; 48kD(Calculated).
Uniprot: P35900
RRID: AB_2845370

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(100%), Sheep(83%), Dog(80%)
Clonality:
Polyclonal
Specificity:
Phospho-Cytokeratin 20 (Ser13) Antibody detects endogenous levels of Cytokeratin 20 only when phosphorylated at Ser13.
RRID:
AB_2845370
Cite Format: Affinity Biosciences Cat# AF2356, RRID:AB_2845370.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD20; CK 20; CK-20; CK20; Cytokeratin-20; Cytokeratin20; K1C20_HUMAN; K20; KA20; Keratin 20; keratin 20, type I; keratin 21, rat, homolog of; Keratin; Keratin type I cytoskeletal 20; Keratin-20; Keratin20; KRT 20; KRT 21; KRT20; KRT21; MGC35423; OTTHUMP00000164518; Protein IT; type I cytoskeletal 20;

Immunogens

Immunogen:
Uniprot:

P35900(K1C20_HUMAN) >>Visit HPA database.

Gene(ID):
KRT20(54474)
Expression:
P35900 K1C20_HUMAN:

Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.

Sequence:
MDFSRRSFHRSLSSSLQAPVVSTVGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMAMQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
83
Dog
80
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35900 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
S13 Phosphorylation P49137 (MAPKAPK2) , P17252 (PRKCA)
S34 Phosphorylation
Y36 Phosphorylation
R42 Methylation
Y84 Phosphorylation
S94 Phosphorylation
S118 Phosphorylation
Y120 Phosphorylation
Y121 Phosphorylation
K149 Ubiquitination
T168 Phosphorylation
T369 Phosphorylation
Y370 Phosphorylation
Y384 Phosphorylation
S387 Phosphorylation
T402 Phosphorylation

Research Backgrounds

Function:

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).

PTMs:

Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).

Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Associates with KRT8.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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