Phospho-EIF3B (Ser154) Antibody | Affinity Biosciences

IHC
pElisa

1/4

AF2352 at 1/100 staining Mouse spleen tissue by IHC-P.

AF2352 at 1/100 staining Mouse brain tissue by IHC-P.

AF2352 at 1/100 staining Rat colorectal tissue by IHC-P.

peptide-ELISA analysis of AF2352.

Product:	Phospho-EIF3B (Ser154) Antibody
Catalog:	AF2352
Description:	Rabbit polyclonal antibody to Phospho-EIF3B (Ser154)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	116 kDa; 92kD(Calculated).
Uniprot:	P55884
RRID:	AB_2845366

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

Phospho-EIF3B (Ser154) Antibody detects endogenous levels of EIF3B only when phosphorylated at Ser154.

RRID:

AB_2845366
Cite Format: Affinity Biosciences Cat# AF2352, RRID:AB_2845366.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

eIF-3-eta; eIF3 eta; eIF3 p110; eIF3 p116; EIF3-ETA; EIF3-P110; EIF3-P116; eIF3b; EIF3B_HUMAN; EIF3S9; EIF3S9, formerly; Eukaryotic translation initiation factor 3, subunit B; Eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3 subunit 9 eta; Eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD); eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa; eukaryotic translation initiation factor 3, subunit 9, formerly; hPrt1; Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of; PRT1; Prt1 homolog;

Immunogens

Immunogen:

Uniprot:

P55884(EIF3B_HUMAN) >>Visit HPA database.

Gene(ID):

EIF3B(8662)

Sequence:

P55884 EIF3B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQDAENVAVPEAAEERAEPGQQQPAAEPPPAEGLLRPAGPGAPEAAGTEASSEEVGIAEAGPESEVRTEPAAEAEAASGPSESPSPPAAEELPGSHAEPPVPAQGEAPGEQARDERSDSRAQAVSEDAGGNEGRAAEAEPRALENGDADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKIFSKFGKITNDFYPEEDGKTKGYIFLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWDIPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLATFHQRGIALWGGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGHKKRGFHCESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIPARVTLMQLPTRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRTPKGTQGVVTNFEIFRMREKQVPVDVVEMKETIIAFAWEPNGSKFAVLHGEAPRISVSFYHVKNNGKIELIKMFDKQQANTIFWSPQGQFVVLAGLRSMNGALAFVDTSDCTVMNIAEHYMASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFCQLLWRPRPPTLLSQEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRKYRKMAQELYMEQKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEIIPLGNQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Horse

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55884 As Substrate

P55884

Site	PTM Type	Source
M1	Acetylation	Uniprot
T68	Phosphorylation	Uniprot
S78	Phosphorylation	Uniprot
S81	Phosphorylation	Uniprot
S83	Phosphorylation	Uniprot
S85	Phosphorylation	Uniprot
S95	Phosphorylation	Uniprot
S117	Phosphorylation	Uniprot
S119	Phosphorylation	Uniprot
S125	Phosphorylation	Uniprot
S152	Phosphorylation	Uniprot
S154	Phosphorylation	Uniprot
S164	Phosphorylation	Uniprot
K202	Ubiquitination	Uniprot
K204	Ubiquitination	Uniprot
K209	Acetylation	Uniprot
K209	Ubiquitination	Uniprot
K213	Acetylation	Uniprot
K213	Ubiquitination	Uniprot
K216	Ubiquitination	Uniprot
T218	Phosphorylation	Uniprot
K228	Ubiquitination	Uniprot
T229	Phosphorylation	Uniprot
K230	Sumoylation	Uniprot
K230	Ubiquitination	Uniprot
Y232	Phosphorylation	Uniprot
Y237	Phosphorylation	Uniprot
S239	Phosphorylation	Uniprot
K248	Ubiquitination	Uniprot
Y253	Phosphorylation	Uniprot
K254	Ubiquitination	Uniprot
K257	Ubiquitination	Uniprot
T267	Phosphorylation	Uniprot
Y272	Phosphorylation	Uniprot
K284	Ubiquitination	Uniprot
K288	Acetylation	Uniprot
K288	Ubiquitination	Uniprot
S312	Phosphorylation	Uniprot
K324	Ubiquitination	Uniprot
Y339	Phosphorylation	Uniprot
K345	Ubiquitination	Uniprot
K364	Acetylation	Uniprot
K364	Ubiquitination	Uniprot
T390	Phosphorylation	Uniprot
S392	Phosphorylation	Uniprot
T397	Phosphorylation	Uniprot
R416	Methylation	Uniprot
K436	Acetylation	Uniprot
S447	Phosphorylation	Uniprot
Y449	Phosphorylation	Uniprot
K459	Ubiquitination	Uniprot
K460	Ubiquitination	Uniprot
S461	Phosphorylation	Uniprot
K463	Ubiquitination	Uniprot
S465	Phosphorylation	Uniprot
S473	Phosphorylation	Uniprot
K487	Ubiquitination	Uniprot
T500	Phosphorylation	Uniprot
K516	Ubiquitination	Uniprot
K521	Ubiquitination	Uniprot
Y525	Phosphorylation	Uniprot
K529	Ubiquitination	Uniprot
K535	Ubiquitination	Uniprot
T537	Phosphorylation	Uniprot
K552	Ubiquitination	Uniprot
S575	Phosphorylation	Uniprot
K599	Ubiquitination	Uniprot
K604	Ubiquitination	Uniprot
K608	Ubiquitination	Uniprot
K693	Ubiquitination	Uniprot
T710	Phosphorylation	Uniprot
S713	Phosphorylation	Uniprot
K718	Ubiquitination	Uniprot
K726	Ubiquitination	Uniprot
S728	Phosphorylation	Uniprot
K729	Acetylation	Uniprot
K729	Ubiquitination	Uniprot
K734	Ubiquitination	Uniprot
S738	Phosphorylation	Uniprot
K741	Ubiquitination	Uniprot
K744	Acetylation	Uniprot
K744	Ubiquitination	Uniprot
T752	Phosphorylation	Uniprot
R758	Methylation	Uniprot
K762	Ubiquitination	Uniprot
Y768	Phosphorylation	Uniprot
K772	Ubiquitination	Uniprot

Research Backgrounds

P55884_EIF3B_HUMAN

Function:

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

(Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.

PTMs:

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with UPF2 and HNRPD. Interacts with METTL3.

Family&Domains:

The RRM domain mediates interaction with EIF3J.

Belongs to the eIF-3 subunit B family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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Phospho-EIF3B (Ser154) Antibody - #AF2352