Phospho-GAB1 (Tyr307) Antibody - #AF2345

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Product: Phospho-GAB1 (Tyr307) Antibody
Catalog: AF2345
Description: Rabbit polyclonal antibody to Phospho-GAB1 (Tyr307)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 115kDa; 77kD(Calculated).
Uniprot: Q13480
RRID: AB_2845359

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
Phospho-GAB1 (Tyr307) Antibody detects endogenous levels of GAB1 only when phosphorylated at Tyr307.
RRID:
AB_2845359
Cite Format: Affinity Biosciences Cat# AF2345, RRID:AB_2845359.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gab 1; GAB1; GAB1_HUMAN; GRB 2 associated binder 1; GRB 2 associated binding protein 1; GRB2 associated binding protein 1 isoform a; GRB2 associated binding protein 1 isoform b; GRB2-associated binder 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1;

Immunogens

Immunogen:
Uniprot:

Q13480(GAB1_HUMAN) >>Visit HPA database.

Gene(ID):
GAB1(2549)
Sequence:
MSGGEVVCSGWLRKSPPEKKLKRYAWKRRWFVLRSGRLTGDPDVLEYYKNDHAKKPIRIIDLNLCQQVDAGLTFNKKEFENSYIFDINTIDRIFYLVADSEEEMNKWVRCICDICGFNPTEEDPVKPPGSSLQAPADLPLAINTAPPSTQADSSSATLPPPYQLINVPPHLETLGIQEDPQDYLLLINCQSKKPEPTRTHADSAKSTSSETDCNDNVPSHKNPASSQSKHGMNGFFQQQMIYDSPPSRAPSASVDSSLYNLPRSYSHDVLPKVSPSSTEADGELYVFNTPSGTSSVETQMRHVSISYDIPPTPGNTYQIPRTFPEGTLGQTSKLDTIPDIPPPRPPKPHPAHDRSPVETCSIPRTASDTDSSYCIPTAGMSPSRSNTISTVDLNKLRKDASSQDCYDIPRAFPSDRSSSLEGFHNHFKVKNVLTVGSVSSEELDENYVPMNPNSPPRQHSSSFTEPIQEANYVPMTPGTFDFSSFGMQVPPPAHMGFRSSPKTPPRRPVPVADCEPPPVDRNLKPDRKVKPAPLEIKPLPEWEELQAPVRSPITRSFARDSSRFPMSPRPDSVHSTTSSSDSHDSEENYVPMNPNLSSEDPNLFGSNSLDGGSSPMIKPKGDKQVEYLDLDLDSGKSTPPRKQKSSGSGSSVADERVDYVVVDQQKTLALKSTREAWTDGRQSTESETPAKSVK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
92
Chicken
92
Rabbit
92
Xenopus
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13480 As Substrate

Site PTM Type Enzyme
S2 Acetylation
Y47 Phosphorylation
Y48 Phosphorylation
Y83 Phosphorylation
Y183 Phosphorylation
K205 Ubiquitination
C213 S-Nitrosylation
K221 Ubiquitination
Y242 Phosphorylation P12931 (SRC) , P06213 (INSR)
S244 Phosphorylation
S251 Phosphorylation
S253 Phosphorylation
S256 Phosphorylation
S257 Phosphorylation
Y259 Phosphorylation P12931 (SRC)
Y265 Phosphorylation
S266 Phosphorylation
S274 Phosphorylation
Y285 Phosphorylation P00533 (EGFR) , P06213 (INSR) , P08581 (MET)
S304 Phosphorylation
S306 Phosphorylation
Y307 Phosphorylation P00533 (EGFR) , P08581 (MET)
T312 Phosphorylation P28482 (MAPK1)
T316 Phosphorylation
Y317 Phosphorylation P12931 (SRC)
S355 Phosphorylation
T365 Phosphorylation
S367 Phosphorylation
T369 Phosphorylation
S371 Phosphorylation
S372 Phosphorylation
Y373 Phosphorylation P08581 (MET) , P06213 (INSR) , P00533 (EGFR) , P12931 (SRC)
S381 Phosphorylation P28482 (MAPK1)
S383 Phosphorylation
T387 Phosphorylation
S389 Phosphorylation
S401 Phosphorylation
S402 Phosphorylation
Y406 Phosphorylation P00533 (EGFR) , P08581 (MET)
S417 Phosphorylation
S418 Phosphorylation
S419 Phosphorylation
Y447 Phosphorylation P06213 (INSR) , P08581 (MET) , P00533 (EGFR)
S454 Phosphorylation P28482 (MAPK1)
Y472 Phosphorylation P00533 (EGFR) , P06213 (INSR) , P08581 (MET)
T476 Phosphorylation P28482 (MAPK1)
S500 Phosphorylation
T503 Phosphorylation
S551 Phosphorylation
S582 Phosphorylation P28482 (MAPK1)
Y589 Phosphorylation P06213 (INSR) , P00533 (EGFR) , P08581 (MET)
S597 Phosphorylation P28482 (MAPK1)
Y627 Phosphorylation P06213 (INSR) , P12931 (SRC) , P08581 (MET) , P09619 (PDGFRB) , P00533 (EGFR)
S634 Phosphorylation
S637 Phosphorylation
T638 Phosphorylation
S645 Phosphorylation
S651 Phosphorylation
Y659 Phosphorylation P08581 (MET) , P06213 (INSR) , P00533 (EGFR) , P09619 (PDGFRB)
T673 Phosphorylation
S683 Phosphorylation
T684 Phosphorylation

Research Backgrounds

Function:

Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway.

PTMs:

Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.

Subunit Structure:

Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK. Part of a tripartite complex containing GAB1, METTL13 and SPRY2. Interacts with METTL13.

Family&Domains:

Belongs to the GAB family.

Restrictive clause

 

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