Phospho-DARPP32 (Ser97)[Ser102] Antibody

WB
pElisa

1/2

Western blot analysis of extracts from Serum-starved EC304 cells, using Phospho-DARPP-32 (Ser97) Antibody.

Product:	Phospho-DARPP32 (Ser97)[Ser102] Antibody
Catalog:	AF2336
Description:	Rabbit polyclonal antibody to Phospho-DARPP32 (Ser97)[Ser102]
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	32kDa; 23kD(Calculated).
Uniprot:	Q9UD71
RRID:	AB_2845350

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%)

Clonality:

Polyclonal

Specificity:

Phospho-DARPP32 (Ser97) Antibody detects endogenous levels of DARPP32 only when phosphorylated at Ser102, which site historically referenced as Ser97.

RRID:

AB_2845350
Cite Format: Affinity Biosciences Cat# AF2336, RRID:AB_2845350.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DARPP 32; DARPP-32; Dopamine and cAMP regulated neuronal phosphoprotein 32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32; Dopamine and cAMP regulated phosphoprotein DARPP32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; PPR1B_HUMAN; Protein phosphatase 1 regulatory (inhibitor) subunit 1B; Protein phosphatase 1 regulatory inhibitor subunit 1B; Protein phosphatase 1 regulatory subunit 1B;

Immunogens

Immunogen:

Uniprot:

Q9UD71(PPR1B_HUMAN) >>Visit HPA database.

Gene(ID):

PPP1R1B(84152)

Sequence:

Q9UD71 PPR1B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPKDRKKIQFSVPAPPSQLDPRQVEMIRRRRPTPAMLFRLSEHSSPEEEASPHQRASGEGHHLKSKRPNPCAYTPPSLKAVQRIAESHLQSISNLNENQASEEEDELGELRELGYPREEDEEEEEDDEEEEEEEDSQAEVLKVIRQSAGQKTTCGQGLEGPWERPPPLDESERDGGSEDQVEDPALSEPGEEPQRPSPSEPGT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Bovine

Sheep

Dog

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UD71 As Substrate

Q9UD71

Site	PTM Type	Enzyme	Source
S12	Phosphorylation		Uniprot
S18	Phosphorylation		Uniprot
T34	Phosphorylation	P17612 (PRKACA)	Uniprot
S45	Phosphorylation		Uniprot
S46	Phosphorylation		Uniprot
S52	Phosphorylation		Uniprot
S58	Phosphorylation		Uniprot
Y74	Phosphorylation		Uniprot
T75	Phosphorylation	Q00535 (CDK5)	Uniprot
S78	Phosphorylation		Uniprot
S102	Phosphorylation		Uniprot
Y116	Phosphorylation		Uniprot
S137	Phosphorylation		Uniprot
S148	Phosphorylation		Uniprot
S172	Phosphorylation		Uniprot
S178	Phosphorylation		Uniprot
S188	Phosphorylation		Uniprot

Research Backgrounds

Q9UD71_PPR1B_HUMAN

Function:

Inhibitor of protein-phosphatase 1.

PTMs:

Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.

Phosphorylation of Thr-34 is required for activity.

Subcellular Location:

Cytoplasm.

Family&Domains:

Belongs to the protein phosphatase inhibitor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Nervous system > Dopaminergic synapse.

Restrictive clause

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For Research Use Only.
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Phospho-DARPP32 (Ser97)[Ser102] Antibody - #AF2336