Phospho-Caveolin 1 (Tyr14) Antibody

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EPG inhibited apoptosis and decreased STIM via p-caveolin-1 against MI injury in myocardium of rats (n = 4-6/group).

Product:	Phospho-Caveolin 1 (Tyr14) Antibody
Catalog:	AF3386
Description:	Rabbit polyclonal antibody to Phospho-Caveolin 1 (Tyr14)
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	23kDa; 20kD(Calculated).
Uniprot:	Q03135
RRID:	AB_2834817

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-Caveolin 1 (Tyr14) Antibody detects endogenous levels of Caveolin 1 only when phosphorylated at Tyrosine 14.

RRID:

AB_2834817
Cite Format: Affinity Biosciences Cat# AF3386, RRID:AB_2834817.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BSCL3; CAV; CAV1; CAV1_HUMAN; caveolae protein, 22 kD; caveolin 1 alpha isoform; caveolin 1 beta isoform; Caveolin 1 caveolae protein 22kDa; Caveolin-1; Caveolin1; cell growth-inhibiting protein 32; CGL3; LCCNS; MSTP085; OTTHUMP00000025031; PPH3; VIP 21; VIP21;

Immunogens

Immunogen:

Uniprot:

Q03135(CAV1_HUMAN) >>Visit HPA database.

Gene(ID):

CAV1(857)

Expression:

Q03135 CAV1_HUMAN:

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

Description:

caveolin-1 May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation.

Sequence:

Q03135 CAV1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGGKYVDSEGHLYTVPIREQGNIYKPNNKAMADELSEKQVYDAHTKEIDLVNRDPKHLNDDVVKIDFEDVIAEPEGTHSFDGIWKASFTTFTVTKYWFYRLLSALFGIPMALIWGIYFAILSFLHIWAVVPCIKSFLIEIQCISRVYSIYVHTVCDPLFEAVGKIFSNVRINLQKEI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03135 As Substrate

Q03135

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
S2	Phosphorylation		Uniprot
K5	Acetylation		Uniprot
K5	Ubiquitination		Uniprot
Y6	Phosphorylation		Uniprot
S9	Phosphorylation		Uniprot
Y14	Phosphorylation	P12931 (SRC) , P06241 (FYN) , P00519 (ABL1)	Uniprot
T15	Phosphorylation		Uniprot
Y25	Phosphorylation		Uniprot
K26	Sumoylation		Uniprot
K26	Ubiquitination		Uniprot
K30	Ubiquitination		Uniprot
S37	Phosphorylation		Uniprot
K39	Ubiquitination		Uniprot
Y42	Phosphorylation		Uniprot
T46	Phosphorylation		Uniprot
K47	Ubiquitination		Uniprot
K57	Acetylation		Uniprot
K57	Ubiquitination		Uniprot
K65	Ubiquitination		Uniprot
S80	Phosphorylation		Uniprot
S88	Phosphorylation		Uniprot
S168	Phosphorylation		Uniprot
K176	Ubiquitination		Uniprot

Research Backgrounds

Q03135_CAV1_HUMAN

Function:

May act as a scaffolding protein within caveolar membranes. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation.

PTMs:

Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation.

The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.

Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

Subcellular Location:

Golgi apparatus membrane>Peripheral membrane protein. Cell membrane>Peripheral membrane protein. Membrane>Caveola>Peripheral membrane protein. Membrane raft. Golgi apparatus>trans-Golgi network.
Note: Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.

Tissue Specificity:

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

Subunit Structure:

Homooligomer. Interacts with GLIPR2. Interacts with NOSTRIN. Interacts with SNAP25 and STX1A (By similarity). Interacts (via the N-terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with PACSIN2; this interaction induces membrane tubulation (By similarity). Interacts with SLC7A9 (By similarity). Interacts with BMX and BTK. Interacts with TGFBR1. Interacts with CAVIN3 (via leucine-zipper domain) in a cholesterol-sensitive manner. Interacts with CAVIN1. Interacts with EHD2 in a cholesterol-dependent manner. Forms a ternary complex with UBXN6 and VCP; mediates CAV1 targeting to lysosomes for degradation. Interacts with ABCG1; this interaction regulates ABCG1-mediated cholesterol efflux.

(Microbial infection) Interacts with rotavirus A NSP4.

Family&Domains:

Belongs to the caveolin family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

References

1). The purified extract of steamed Panax ginseng protects cardiomyocyte from ischemic injury via caveolin-1 phosphorylation-mediating calcium influx. Journal of Ginseng Research, 2023 (PubMed: 38107394) [IF=6.3]

Application: IF/ICC Species: Rat Sample:

EPG inhibited apoptosis and decreased STIM via p-caveolin-1 against MI injury in myocardium of rats (n = 4-6/group). (A) TUNEL staining, apoptotic nuclei (green) and DAPI-positive normal nuclei (blue). (B) Immuno-histochemical staining of p-caveolin-1 and STIM1. (C) Apoptotic rate (%). (D, E) Quantitative mean fluorescence intensity (MFI). ###P < 0.001 vs. sham, ∗∗∗P < 0.001 vs. model, & P < 0.05, &&&P < 0.001 vs. EPG.

Application: WB Species: Rat Sample:

Fig. 5 P-Caveolin-1/SOCE pathway mediated EPG-induced protection against MI injury in rats (n = 4-6/group). (A) Western blot bands. Quantitative analysis of protein expression (B–F) and mRNA expression (G-J).

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For Research Use Only.
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Phospho-Caveolin 1 (Tyr14) Antibody - #AF3386