Phospho-BAP1 (Ser592) Antibody - #AF2321

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Product: Phospho-BAP1 (Ser592) Antibody
Catalog: AF2321
Description: Rabbit polyclonal antibody to Phospho-BAP1 (Ser592)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Bovine, Horse, Sheep
Mol.Wt.: 95kDa; 80kD(Calculated).
Uniprot: Q92560
RRID: AB_2845335

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Bovine(83%), Horse(80%), Sheep(83%)
Clonality:
Polyclonal
Specificity:
Phospho-BAP1 (Ser592) Antibody detects endogenous levels of BAP1 only when phosphorylated at Ser592.
RRID:
AB_2845335
Cite Format: Affinity Biosciences Cat# AF2321, RRID:AB_2845335.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BAP 1; Bap1; BAP1_HUMAN; BRCA 1 associated protein 1; BRCA1 associated protein 1; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); BRCA1-associated protein 1; Cerebral protein 13; Cerebral protein 6; DKFZp686N04275; FLJ35406; FLJ37180; HUCEP 13; Hucep 6; HUCEP13; Hucep6; KIAA0272; TPDS; Ubiquitin carboxy terminal hydrolase; Ubiquitin carboxyl terminal hydrolase BAP 1; Ubiquitin carboxyl terminal hydrolase BAP1; Ubiquitin carboxyl-terminal hydrolase BAP1; UCHL2;

Immunogens

Immunogen:
Uniprot:

Q92560(BAP1_HUMAN) >>Visit HPA database.

Gene(ID):
BAP1(8314)
Expression:
Q92560 BAP1_HUMAN:

Highly expressed in testis, placenta and ovary. Expressed in breast.

Sequence:
MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDTSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSSVDLGPTLSRMKDFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMERIGLATAGEPYHDIRFNLMAVVPDRRIKYEARLHVLKVNRQTVLEALQQLIRVTQPELIQTHKSQESQLPEESKSASNKSPLVLEANRAPAASEGNHTDGAEEAAGSCAQAPSHSPPNKPKLVVKPPGSSLNGVHPNPTPIVQRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRVPVRPPQQYSDDEDDYEDDEEDDVQNTNSALRYKGKGTGKPGALSGSADGQLSVLQPNTINVLAEKLKESQKDLSIPLSIKTSSGAGSPAVAVPTHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHISKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGLLHLAEDGVLSPLALTEGGKGSSPSIRPIQGSQGSSSPVEKEVVEATDSREKTGMVRPGEPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
83
Sheep
83
Horse
80
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92560 As Substrate

Site PTM Type Enzyme
K120 Ubiquitination
S123 Phosphorylation
K127 Ubiquitination
K139 Ubiquitination
K155 Acetylation
K155 Ubiquitination
Y223 Phosphorylation
Y241 Phosphorylation
T273 Phosphorylation
K275 Ubiquitination
S276 Phosphorylation
S287 Phosphorylation
S289 Phosphorylation
S292 Phosphorylation
S325 Phosphorylation
S327 Phosphorylation
S369 Phosphorylation
S384 Phosphorylation
Y394 Phosphorylation
S395 Phosphorylation
Y401 Phosphorylation
S460 Phosphorylation
S464 Phosphorylation
S485 Phosphorylation
T487 Phosphorylation
S489 Phosphorylation
T493 Phosphorylation
S505 Phosphorylation
S509 Phosphorylation
S513 Phosphorylation
T517 Phosphorylation
S520 Phosphorylation
S521 Phosphorylation
T524 Phosphorylation
S525 Phosphorylation
S537 Phosphorylation
S571 Phosphorylation
S582 Phosphorylation
S583 Phosphorylation
S585 Phosphorylation
S592 Phosphorylation
S595 Phosphorylation
S596 Phosphorylation
S597 Phosphorylation
S609 Phosphorylation
K630 Ubiquitination
K656 Methylation
K658 Methylation
K659 Methylation
S705 Phosphorylation

Research Backgrounds

Function:

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor.

PTMs:

Ubiquitinated: monoubiquitinated at multiple site of its nuclear localization signal (NLS) BY UBE2O, leading to cytoplasmic retention. Able to mediate autodeubiquitination via intramolecular interactions to couteract cytoplasmic retention.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O (PubMed:24703950).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis, placenta and ovary. Expressed in breast.

Subunit Structure:

Component of the PR-DUB complex, at least composed of BAP1 and ASXL1. Interacts with BRCA1 (via the RING finger). Interacts (via HBM-like motif) with HCFC1. Interacts (when phosphorylated at Thr-493) with FOXK1. Interacts (when phosphorylated at Thr-493) with FOXK2; leading to recruit the PR-DUB complex and repress FOXK2 target genes.

Family&Domains:

Belongs to the peptidase C12 family. BAP1 subfamily.

Restrictive clause

 

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