Phospho-Atg13 (Ser355) Antibody | Affinity Biosciences

WB
IHC
pElisa
Cites

1/5

Western blot analysis of extracts from Serum-starved EC304 cells, using Phospho-Atg13 (Ser355) Antibody.

Western blot analysis of extracts from Mouse testis, using Phospho-Atg13 (Ser355) Antibody at 1/1000 dilution.

AF2319 at 1/100 staining Mouse spleen tissue by IHC-P.

peptide-ELISA analysis of AF2319.

FIG.

Product:	Phospho-Atg13 (Ser355) Antibody
Catalog:	AF2319
Description:	Rabbit polyclonal antibody to Phospho-Atg13 (Ser355)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	72kDa; 57kD(Calculated).
Uniprot:	O75143
RRID:	AB_2845333

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(90%)

Clonality:

Polyclonal

Specificity:

Phospho-Atg13 (Ser355) Antibody detects endogenous levels of Atg13 only when phosphorylated at Ser355.

RRID:

AB_2845333
Cite Format: Affinity Biosciences Cat# AF2319, RRID:AB_2845333.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATG13; ATG13 autophagy related 13 homolog (S. cerevisiae); ATG13_HUMAN; Autophagy related 13; Autophagy related protein 13; Autophagy-related protein 13; FLJ20698; KIAA0652; OTTHUMP00000233321; OTTHUMP00000233322; OTTHUMP00000233323; OTTHUMP00000233324; OTTHUMP00000233325; OTTHUMP00000233326;

Immunogens

Immunogen:

Uniprot:

O75143(ATG13_HUMAN) >>Visit HPA database.

Gene(ID):

ATG13(9776)

Sequence:

O75143 ATG13_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

METDLNSQDRKDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDWFNLAIKDIPEVTHEAKKALAGQLPAVGRSMCVEISLKTSEGDSMELEIWCLEMNEKCDKEIKVSYTVYNRLSLLLKSLLAITRVTPAYRLSRKQGHEYVILYRIYFGEVQLSGLGEGFQTVRVGTVGTPVGTITLSCAYRINLAFMSTRQFERTPPIMGIIIDHFVDRPYPSSSPMHPCNYRTAGEDTGVIYPSVEDSQEVCTTSFSTSPPSQLSSSRLSYQPAALGVGSADLAYPVVFAAGLNATHPHQLMVPGKEGGVPLAPNQPVHGTQADQERLATCTPSDRTHCAATPSSSEDTETVSNSSEGRASPHDVLETIFVRKVGAFVNKPINQVTLTSLDIPFAMFAPKNLELEDTDPMVNPPDSPETESPLQGSLHSDGSSGGSSGNTHDDFVMIDFKPAFSKDDILPMDLGTFYREFQNPPQLSSLSIDIGAQSMAEDLDSLPEKLAVHEKNVREFDAFVETLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Rabbit

100

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75143 As Substrate

O75143

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S7	Phosphorylation		Uniprot
T24	Phosphorylation		Uniprot
K56	Ubiquitination		Uniprot
S141	Phosphorylation		Uniprot
K143	Ubiquitination		Uniprot
T170	Phosphorylation		Uniprot
T204	Phosphorylation		Uniprot
T332	Phosphorylation		Uniprot
S334	Phosphorylation		Uniprot
S355	Phosphorylation	O75385 (ULK1)	Uniprot
S356	Phosphorylation		Uniprot
S361	Phosphorylation		Uniprot
S389	Phosphorylation		Uniprot
K504	Ubiquitination		Uniprot

Research Backgrounds

O75143_ATG13_HUMAN

Function:

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

PTMs:

Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria.

Subcellular Location:

Cytoplasm>Cytosol. Preautophagosomal structure.
Note: Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.

Subunit Structure:

Part of a complex consisting of ATG13, ULK1 and RB1CC1. Interacts with ATG101. Interacts with ULK1 (via C-terminus). Interacts with ULK2 (via C-terminus). Interacts (via the LIR motif) with GABARAP, GABARAPL, GABARAPL2. Interacts (via the LIR motif) with MAP1LC3A, MAP1LC3B and MAP1LC3C. Interacts with TAB2 and TAB3. Interacts with C9orf72.

Family&Domains:

The LIR motif (LC3-interacting region) is required for the interaction with the ATG8 family proteins GABARAP, GABARAPL, GABARAPL2, and MAP1LC3A.

Belongs to the ATG13 family. Metazoan subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

References

1). Mechanisms of mTOR and Autophagy in Human Endothelial Cell Infected with Dengue Virus-2. VIRAL IMMUNOLOGY (PubMed: 31978319) [IF=2.2]

Application: WB Species: human Sample: HUVECs

FIG. 5. |Analysis of expression of LC3-II, the phosphorylation levels of Atg13 (p-Atg13), and the phosphorylation levels of ULK1 (p-ULK1) in HUVECs infected with DENV-2 or treated with rapamycin (RAPA) for 24 h. (A): Expression of the LC3-II, p- Atg13, p-ULK1, and GAPDH protein in infected cells at virus titer (1*104 TCID50) or RAPA treatment analyzed by western blot. Proteins were harvested at 24 h.

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Phospho-Atg13 (Ser355) Antibody - #AF2319