Phospho-Atg13 (Ser355) Antibody - #AF2319

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Product: Phospho-Atg13 (Ser355) Antibody
Catalog: AF2319
Description: Rabbit polyclonal antibody to Phospho-Atg13 (Ser355)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 72kDa; 57kD(Calculated).
Uniprot: O75143
RRID: AB_2845333

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
Phospho-Atg13 (Ser355) Antibody detects endogenous levels of Atg13 only when phosphorylated at Ser355.
RRID:
AB_2845333
Cite Format: Affinity Biosciences Cat# AF2319, RRID:AB_2845333.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATG13; ATG13 autophagy related 13 homolog (S. cerevisiae); ATG13_HUMAN; Autophagy related 13; Autophagy related protein 13; Autophagy-related protein 13; FLJ20698; KIAA0652; OTTHUMP00000233321; OTTHUMP00000233322; OTTHUMP00000233323; OTTHUMP00000233324; OTTHUMP00000233325; OTTHUMP00000233326;

Immunogens

Immunogen:
Uniprot:

O75143(ATG13_HUMAN) >>Visit HPA database.

Gene(ID):
ATG13(9776)
Sequence:
METDLNSQDRKDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDWFNLAIKDIPEVTHEAKKALAGQLPAVGRSMCVEISLKTSEGDSMELEIWCLEMNEKCDKEIKVSYTVYNRLSLLLKSLLAITRVTPAYRLSRKQGHEYVILYRIYFGEVQLSGLGEGFQTVRVGTVGTPVGTITLSCAYRINLAFMSTRQFERTPPIMGIIIDHFVDRPYPSSSPMHPCNYRTAGEDTGVIYPSVEDSQEVCTTSFSTSPPSQLSSSRLSYQPAALGVGSADLAYPVVFAAGLNATHPHQLMVPGKEGGVPLAPNQPVHGTQADQERLATCTPSDRTHCAATPSSSEDTETVSNSSEGRASPHDVLETIFVRKVGAFVNKPINQVTLTSLDIPFAMFAPKNLELEDTDPMVNPPDSPETESPLQGSLHSDGSSGGSSGNTHDDFVMIDFKPAFSKDDILPMDLGTFYREFQNPPQLSSLSIDIGAQSMAEDLDSLPEKLAVHEKNVREFDAFVETLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
92
Chicken
90
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75143 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S7 Phosphorylation
T24 Phosphorylation
K56 Ubiquitination
S141 Phosphorylation
K143 Ubiquitination
T170 Phosphorylation
T204 Phosphorylation
T332 Phosphorylation
S334 Phosphorylation
S355 Phosphorylation O75385 (ULK1)
S356 Phosphorylation
S361 Phosphorylation
S389 Phosphorylation
K504 Ubiquitination

Research Backgrounds

Function:

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

PTMs:

Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria.

Subcellular Location:

Cytoplasm>Cytosol. Preautophagosomal structure.
Note: Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Part of a complex consisting of ATG13, ULK1 and RB1CC1. Interacts with ATG101. Interacts with ULK1 (via C-terminus). Interacts with ULK2 (via C-terminus). Interacts (via the LIR motif) with GABARAP, GABARAPL, GABARAPL2. Interacts (via the LIR motif) with MAP1LC3A, MAP1LC3B and MAP1LC3C. Interacts with TAB2 and TAB3. Interacts with C9orf72.

Family&Domains:

The LIR motif (LC3-interacting region) is required for the interaction with the ATG8 family proteins GABARAP, GABARAPL, GABARAPL2, and MAP1LC3A.

Belongs to the ATG13 family. Metazoan subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

References

1). Low selenium and T-2 toxin may be involved in the pathogenesis of Kashin-Beck disease by affecting AMPK/mTOR/ULK1 pathway mediated autophagy. Ecotoxicology and environmental safety, 2024 (PubMed: 38810288) [IF=6.8]

Application: WB    Species: Rat    Sample:

Fig. 2. mRNA and proteins expressions of AMPK/mTOR/ULK1 pathway and autophagy markers in chondrocytes from normal control (NC) and Kashin-beck disease (KBD). A. mRNA expression of AMPK, mTOR, ULK1, and ATG13 in NC and KBD chondrocytes. n≥4. B. mRNA expression of LC3B, Beclin1, p62 in NC and KBD chondrocytes. n≥4. C. Proteins expression of p-AMPK, AMPK, p-mTOR, mTOR, p-ULK1, ULK1, p-ATG13, ATG13 in NC and KBD chondrocytes. D. Proteins expression level of autophagy markers (LC3-II/I, Beclin1, p62) in NC and KBD chondrocytes. n=3. The data are shown as mean ± SD for three independent experiments. *Statistically significant difference between NC and KBD (*P
2). Mechanisms of mTOR and Autophagy in Human Endothelial Cell Infected with Dengue Virus-2. VIRAL IMMUNOLOGY, 2020 (PubMed: 31978319) [IF=2.2]

Application: WB    Species: human    Sample: HUVECs

FIG. 5. |Analysis of expression of LC3-II, the phosphorylation levels of Atg13 (p-Atg13), and the phosphorylation levels of ULK1 (p-ULK1) in HUVECs infected with DENV-2 or treated with rapamycin (RAPA) for 24 h. (A): Expression of the LC3-II, p- Atg13, p-ULK1, and GAPDH protein in infected cells at virus titer (1*104 TCID50) or RAPA treatment analyzed by western blot. Proteins were harvested at 24 h.

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