Phospho-AS160 (Ser588) Antibody - #AF2318

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Product: Phospho-AS160 (Ser588) Antibody
Catalog: AF2318
Description: Rabbit polyclonal antibody to Phospho-AS160 (Ser588)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 160kDa; 147kD(Calculated).
Uniprot: O60343
RRID: AB_2845332

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(92%), Rabbit(100%), Dog(92%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
Phospho-AS160 (Ser588) Antibody detects endogenous levels of AS160 only when phosphorylated at Ser588.
RRID:
AB_2845332
Cite Format: Affinity Biosciences Cat# AF2318, RRID:AB_2845332.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acrg embryonic lethality (mouse) minimal region ortholog; Acrg embryonic lethality minimal region ortholog; Acrg embryonic lethality mouse minimal region ortholog; Akt substrate of 160 kDa; AS 160; AS160; BUB2; CDC16; KIAA0603; NIDDM5; TBC (Tre 2 BUB2 CDC16) domain containing protein; TBC Tre 2 BUB2 CDC16 domain containing protein; TBC1 D4; TBC1 domain family member 4; Tbc1d4; TBCD4_HUMAN; Tre-2;

Immunogens

Immunogen:
Uniprot:

O60343(TBCD4_HUMAN) >>Visit HPA database.

Gene(ID):
TBC1D4(9882)
Expression:
O60343 TBCD4_HUMAN:

Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.

Sequence:
MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPSTISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKESNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENEIRQDTASESSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVVFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Chicken
100
Rabbit
100
Horse
92
Dog
92
Xenopus
92
Pig
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60343 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S44 Phosphorylation
S66 Phosphorylation
K68 Ubiquitination
S102 Phosphorylation
K228 Ubiquitination
K238 Ubiquitination
S262 Phosphorylation
T274 Phosphorylation
S285 Phosphorylation
S304 Phosphorylation
S314 Phosphorylation
S317 Phosphorylation
S318 Phosphorylation O00141 (SGK1) , P31749 (AKT1)
T320 Phosphorylation
S330 Phosphorylation
S341 Phosphorylation Q15418 (RPS6KA1)
S344 Phosphorylation
S349 Phosphorylation
S351 Phosphorylation
T356 Phosphorylation
S370 Phosphorylation
K383 Ubiquitination
K477 Acetylation
S485 Phosphorylation O14757 (CHEK1)
S486 Phosphorylation
T488 Phosphorylation
S551 Phosphorylation
K555 Ubiquitination
K560 Ubiquitination
S566 Phosphorylation
T568 Phosphorylation O00141 (SGK1)
S569 Phosphorylation
S570 Phosphorylation
S576 Phosphorylation
S588 Phosphorylation O14757 (CHEK1) , P31749 (AKT1) , O00141 (SGK1)
S591 Phosphorylation
S595 Phosphorylation
S597 Phosphorylation
S600 Phosphorylation
S609 Phosphorylation
T613 Phosphorylation
S617 Phosphorylation
S621 Phosphorylation
S633 Phosphorylation
T642 Phosphorylation O00141 (SGK1) , P31749 (AKT1)
S644 Phosphorylation
S648 Phosphorylation
S649 Phosphorylation
S666 Phosphorylation
S672 Phosphorylation
S673 Phosphorylation
S678 Phosphorylation
T701 Phosphorylation
S704 Phosphorylation
S707 Phosphorylation
T749 Phosphorylation
S750 Phosphorylation
S751 Phosphorylation O00141 (SGK1) , Q15418 (RPS6KA1)
T752 Phosphorylation
S754 Phosphorylation
S757 Phosphorylation
S759 Phosphorylation
T763 Phosphorylation
S764 Phosphorylation
T766 Phosphorylation
S782 Phosphorylation
S787 Phosphorylation
S789 Phosphorylation
S806 Phosphorylation
S809 Phosphorylation
Y1191 Phosphorylation
S1207 Phosphorylation
S1246 Phosphorylation
K1255 Ubiquitination
K1267 Ubiquitination

Research Backgrounds

Function:

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

PTMs:

Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.

Subcellular Location:

Cytoplasm.
Note: Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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