Phospho-GRK2 (Ser29) Antibody | Affinity Biosciences

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Product:	Phospho-GRK2 (Ser29) Antibody
Catalog:	AF2309
Description:	Rabbit polyclonal antibody to Phospho-GRK2 (Ser29)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Dog, Chicken
Mol.Wt.:	80kDa; 80kD(Calculated).
Uniprot:	P25098
RRID:	AB_2845323

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Dog(100%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

Phospho-GRK2 (Ser29) Antibody detects endogenous levels of GRK2 only when phosphorylated at Ser29.

RRID:

AB_2845323
Cite Format: Affinity Biosciences Cat# AF2309, RRID:AB_2845323.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADRBK1; Adrenergic beta receptor kinase 1; ARBK1_HUMAN; BARK; BARK1; Beta adrenergic receptor kinase 1; Beta ARK 1; Beta ARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; FLJ16718; G protein coupled receptor kinase 2; G-protein coupled receptor kinase 2; GRK2;

Immunogens

Immunogen:

Uniprot:

P25098(ARBK1_HUMAN) >>Visit HPA database.

Gene(ID):

GRK2(156)

Expression:

P25098 ARBK1_HUMAN:

Expressed in peripheral blood leukocytes.

Sequence:

P25098 ARBK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Dog

100

Chicken

Pig

Horse

Sheep

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25098 As Substrate

P25098

Site	PTM Type	Enzyme	Source
Y13	Phosphorylation	P00533 (EGFR) , P12931 (SRC)	Uniprot
S29	Phosphorylation	P17252 (PRKCA) , P05129 (PRKCG) , Q05655 (PRKCD)	Uniprot
K31	Ubiquitination		Uniprot
K45	Ubiquitination		Uniprot
R50	Methylation		Uniprot
T54	Phosphorylation		Uniprot
K57	Ubiquitination		Uniprot
K62	Ubiquitination		Uniprot
Y86	Phosphorylation	P00533 (EGFR) , P12931 (SRC)	Uniprot
Y92	Phosphorylation	P12931 (SRC) , P00533 (EGFR)	Uniprot
K94	Ubiquitination		Uniprot
K115	Ubiquitination		Uniprot
K126	Ubiquitination		Uniprot
K138	Ubiquitination		Uniprot
K139	Ubiquitination		Uniprot
K164	Ubiquitination		Uniprot
K170	Ubiquitination		Uniprot
Y206	Phosphorylation		Uniprot
T213	Phosphorylation		Uniprot
K215	Ubiquitination		Uniprot
K220	Ubiquitination		Uniprot
K230	Ubiquitination		Uniprot
S247	Phosphorylation		Uniprot
T248	Phosphorylation		Uniprot
T263	Phosphorylation		Uniprot
K319	Ubiquitination		Uniprot
K344	Ubiquitination		Uniprot
K345	Ubiquitination		Uniprot
K346	Ubiquitination		Uniprot
S350	Phosphorylation		Uniprot
T353	Phosphorylation		Uniprot
Y356	Phosphorylation		Uniprot
S416	Phosphorylation		Uniprot
S423	Phosphorylation		Uniprot
K448	Ubiquitination		Uniprot
S487	Phosphorylation		Uniprot
K494	Ubiquitination		Uniprot
K497	Ubiquitination		Uniprot
S501	Phosphorylation		Uniprot
Y506	Phosphorylation		Uniprot
K545	Ubiquitination		Uniprot
K557	Ubiquitination		Uniprot
K628	Ubiquitination		Uniprot
K644	Acetylation		Uniprot
K644	Ubiquitination		Uniprot
S670	Phosphorylation	P27361 (MAPK3) , P24941 (CDK2)	Uniprot
S676	Phosphorylation		Uniprot
K677	Ubiquitination		Uniprot
R683	Methylation		Uniprot
S685	Phosphorylation	P17612 (PRKACA)	Uniprot

PTMs - P25098 As Enzyme

P25098

Substrate	Site	Source
P05387 (RPLP2)	S102	Uniprot
P05387 (RPLP2)	S105	Uniprot
P07550 (ADRB2)	S355	Uniprot
P07550 (ADRB2)	S356	Uniprot
P07550 (ADRB2)	T384	Uniprot
P07550 (ADRB2)	S396	Uniprot
P07550 (ADRB2)	S401	Uniprot
P07550 (ADRB2)	S407	Uniprot
P07550 (ADRB2)	S411	Uniprot
P08069 (IGF1R)	S1278	Uniprot
P08172 (CHRM2)	S232	Uniprot
P08172 (CHRM2)	S234	Uniprot
P08172 (CHRM2)	S250	Uniprot
P08172 (CHRM2)	S251	Uniprot
P08172 (CHRM2)	T271	Uniprot
P08172 (CHRM2)	S282	Uniprot
P08172 (CHRM2)	S283	Uniprot
P08172 (CHRM2)	S286	Uniprot
P08172 (CHRM2)	T287	Uniprot
P08172 (CHRM2)	S288	Uniprot
P08172 (CHRM2)	S290	Uniprot
P08172 (CHRM2)	S294	Uniprot
P08172 (CHRM2)	T302	Uniprot
P08172 (CHRM2)	T307	Uniprot
P08172 (CHRM2)	S309	Uniprot
P08172 (CHRM2)	T310	Uniprot
P08172 (CHRM2)	S311	Uniprot
P09497 (CLTB)	S205	Uniprot
P15311 (EZR)	T567	Uniprot
P18545 (PDE6G)	T62	Uniprot
P21731-2 (TBXA2R)	S239	Uniprot
P21731-2 (TBXA2R)	S357	Uniprot
P26038 (MSN)	T558	Uniprot
P30411 (BDKRB2)	S366	Uniprot
P30411 (BDKRB2)	S373	Uniprot
P30411 (BDKRB2)	S375	Uniprot
P32245 (MC4R)	T312	Uniprot
P32245 (MC4R)	S329	Uniprot
P32245 (MC4R)	S330	Uniprot
P35241 (RDX)	T564	Uniprot
P35367 (HRH1)	S398	Uniprot
P35372 (OPRM1)	T356	Uniprot
P35372 (OPRM1)	S357	Uniprot
P35372 (OPRM1)	S358	Uniprot
P35372 (OPRM1)	S377	Uniprot
P37840 (SNCA)	S129	Uniprot
P41143 (OPRD1)	T358	Uniprot
P41143 (OPRD1)	S363	Uniprot
Q14940 (SLC9A5)	S702	Uniprot
Q14940 (SLC9A5)	S709	Uniprot
Q14940 (SLC9A5)	S711	Uniprot
Q14940 (SLC9A5)	S712	Uniprot
Q14940 (SLC9A5)	T714	Uniprot
Q15796 (SMAD2)	T197	Uniprot
Q16539 (MAPK14)	T123	Uniprot
Q96A54 (ADIPOR1)	S7	Uniprot
Q96A54 (ADIPOR1)	T24	Uniprot
Q96A54 (ADIPOR1)	T53	Uniprot
Q9Y2W7 (KCNIP3)	S95	Uniprot

Research Backgrounds

P25098_ARBK1_HUMAN

Function:

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity).

Subcellular Location:

Cytoplasm. Cell membrane.

Tissue Specificity:

Expressed in peripheral blood leukocytes.

Subunit Structure:

Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1. Interacts with LPAR1 and LPAR2. Interacts with RALA in response to LPAR1 activation. ADRBK1 and RALA mutually inhibit each other's binding to LPAR1. Interacts with ADRB2.

Family&Domains:

The PH domain binds anionic phospholipids and helps recruiting ADRBK1 from the cytoplasm to plasma membrane close to activated receptors. It mediates binding to G protein beta and gamma subunits, competing with G-alpha subunits and other G-betagamma effectors.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-GRK2 (Ser29) Antibody - #AF2309