ZFYVE16 Antibody - #DF12507

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Product: ZFYVE16 Antibody
Catalog: DF12507
Description: Rabbit polyclonal antibody to ZFYVE16
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 169 kDa,250 kDa; 169kD(Calculated).
Uniprot: Q7Z3T8
RRID: AB_2845312

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(92%), Horse(85%), Sheep(92%), Dog(85%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
ZFYVE16 Antibody detects endogenous levels of total ZFYVE16.
RRID:
AB_2845312
Cite Format: Affinity Biosciences Cat# DF12507, RRID:AB_2845312.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI035632; B130024H06Rik; B130031L15; DKFZp686E13162; Endofin; Endosomal associated FYVE domain protein; Endosome associated FYVE domain protein; Endosome-associated FYVE domain protein; KIAA0305; KIAA0305;; mKIAA0305; OTTMUSP00000029589; RGD1564784; ZFY16_HUMAN; ZFYVE16; Zinc finger FYVE domain containing protein 16; Zinc finger FYVE domain-containing protein 16; Zinc finger, FYVE domain containing 16;

Immunogens

Immunogen:
Uniprot:

Q7Z3T8(ZFY16_HUMAN) >>Visit HPA database.

Gene(ID):
ZFYVE16(9765)
Expression:
Q7Z3T8 ZFY16_HUMAN:

Widely expressed. Highly expressed in kidney, placenta and lung. Expressed at intermediate level in heart, brain, skeletal muscle, spleen and liver. Weakly expressed in colon, thymus and peripheral blood lymphocytes.

Sequence:
MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQECVNSCASSETSYGTNESSLNEKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMGRCSKPICDLISDMGNLVHATNSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDHDSDTVREQQNDISSELQNREIGGIKELGIKVDTTLSDSYNYSGTENLKDKKIFNQLESIVDFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKEEVDVAVITAAECLKEEGKTSALTCSLPKNEDLCLNDSNSRDENFKLPDFSFQEDKTVIKQSAQEDSKSLDLKDNDVIQDSSSALHVSSKDVPSSLSCLPASGSMCGSLIESKARGDFLPQHEHKDNIQDAVTIHEEIQNSVVLGGEPFKENDLLKQEKCKSILLQSLIEGMEDRKIDPDQTVIRAESLDGGDTSSTVVESQEGLSGTHVPESSDCCEGFINTFSSNDMDGQDLDYFNIDEGAKSGPLISDAELDAFLTEQYLQTTNIKSFEENVNDSKSQMNQIDMKGLDDGNINNIYFNAEAGAIGESHGINIICEIVDKQNTIENGLSLGEKSTIPVQQGLPTSKSEITNQLSVSDINSQSVGGARPKQLFSLPSRTRSSKDLNKPDVPDTIESEPSTADTVVPITCAIDSTADPQVSFNSNYIDIESNSEGGSSFVTANEDSVPENTCKEGLVLGQKQPTWVPDSEAPNCMNCQVKFTFTKRRHHCRACGKVFCGVCCNRKCKLQYLEKEARVCVVCYETISKAQAFERMMSPTGSNLKSNHSDECTTVQPPQENQTSSIPSPATLPVSALKQPGVEGLCSKEQKRVWFADGILPNGEVADTTKLSSGSKRCSEDFSPLSPDVPMTVNTVDHSHSTTVEKPNNETGDITRNEIIQSPISQVPSVEKLSMNTGNEGLPTSGSFTLDDDVFAETEEPSSPTGVLVNSNLPIASISDYRLLCDINKYVCNKISLLPNDEDSLPPLLVASGEKGSVPVVEEHPSHEQIILLLEGESFHPVTFVLNANLLVNVKFIFYSSDKYWYFSTNGLHGLGQAEIIILLLCLPNEDTIPKDIFRLFITIYKDALKGKYIENLDNITFTESFLSSKDHGGFLFITPTFQKLDDLSLPSNPFLCGILIQKLEIPWAKVFPMRLMLRLGAEYKAYPAPLTSIRGRKPLFGEIGHTIMNLLVDLRNYQYTLHNIDQLLIHMEMGKSCIKIPRKKYSDVMKVLNSSNEHVISIGASFSTEADSHLVCIQNDGIYETQANSATGHPRKVTGASFVVFNGALKTSSGFLAKSSIVEDGLMVQITPETMNGLRLALREQKDFKITCGKVDAVDLREYVDICWVDAEEKGNKGVISSVDGISLQGFPSEKIKLEADFETDEKIVKCTEVFYFLKDQDLSILSTSYQFAKEIAMACSAALCPHLKTLKSNGMNKIGLRVSIDTDMVEFQAGSEGQLLPQHYLNDLDSALIPVIHGGTSNSSLPLEIELVFFIIEHLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
92
Sheep
92
Horse
85
Dog
85
Chicken
83
Pig
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z3T8 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
Y28 Phosphorylation
Y36 Phosphorylation
Y73 Phosphorylation
K86 Ubiquitination
T89 Phosphorylation
S90 Phosphorylation
K95 Ubiquitination
Y118 Phosphorylation
K124 Ubiquitination
T140 Phosphorylation
S142 Phosphorylation
K148 Ubiquitination
S160 Phosphorylation
S167 Phosphorylation
S168 Phosphorylation
S170 Phosphorylation
K205 Ubiquitination
S216 Phosphorylation
S218 Phosphorylation
Y219 Phosphorylation
Y221 Phosphorylation
K228 Acetylation
K228 Ubiquitination
S253 Phosphorylation
K265 Ubiquitination
S305 Phosphorylation
S317 Phosphorylation
S319 Phosphorylation
K325 Ubiquitination
S330 Phosphorylation
K335 Ubiquitination
S348 Phosphorylation
S367 Phosphorylation
S376 Phosphorylation
S391 Phosphorylation
K392 Ubiquitination
S441 Phosphorylation
S446 Phosphorylation
K455 Ubiquitination
Y515 Phosphorylation
S529 Phosphorylation
Y541 Phosphorylation
K548 Ubiquitination
S549 Phosphorylation
S557 Phosphorylation
K558 Ubiquitination
Y578 Phosphorylation
K614 Ubiquitination
T616 Phosphorylation
K627 Ubiquitination
S635 Phosphorylation
S643 Phosphorylation
K650 Ubiquitination
S654 Phosphorylation
S661 Phosphorylation
K740 Ubiquitination
Y789 Phosphorylation
S815 Phosphorylation
S845 Phosphorylation
T848 Phosphorylation
K855 Ubiquitination
K865 Ubiquitination
S896 Phosphorylation
S903 Phosphorylation
T928 Phosphorylation
T932 Phosphorylation
S939 Phosphorylation
S942 Phosphorylation
S946 Phosphorylation
K1006 Ubiquitination
K1011 Ubiquitination
S1029 Phosphorylation
T1120 Phosphorylation
K1147 Ubiquitination
T1156 Phosphorylation
Y1201 Phosphorylation
K1202 Ubiquitination
Y1204 Phosphorylation
T1209 Phosphorylation
S1210 Phosphorylation
K1257 Ubiquitination
K1336 Ubiquitination
S1337 Phosphorylation
K1364 Ubiquitination
K1367 Ubiquitination
K1372 Ubiquitination
Y1381 Phosphorylation
K1392 Ubiquitination
K1395 Ubiquitination
K1413 Ubiquitination
K1452 Acetylation

Research Backgrounds

Function:

May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.

Subcellular Location:

Cytoplasm. Early endosome membrane>Peripheral membrane protein.
Note: Localized to early endosomes. Membrane-associated, probably via its association with phosphatidylinositol 3-phosphate (PI3P).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Highly expressed in kidney, placenta and lung. Expressed at intermediate level in heart, brain, skeletal muscle, spleen and liver. Weakly expressed in colon, thymus and peripheral blood lymphocytes.

Subunit Structure:

Interacts with the C-terminus of TOM1. Does not interact with TOM1L1 or TOM1L2.

Family&Domains:

The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

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