UFD1L Antibody - #DF12494

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Product: UFD1L Antibody
Catalog: DF12494
Description: Rabbit polyclonal antibody to UFD1L
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: Q92890
RRID: AB_2845299

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Rabbit(92%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
UFD1L Antibody detects endogenous levels of total UFD1L.
RRID:
AB_2845299
Cite Format: Affinity Biosciences Cat# DF12494, RRID:AB_2845299.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

UB fusion protein 1; Ubiquitin fusion degradation 1 like (yeast); Ubiquitin fusion degradation 1 like; Ubiquitin fusion degradation protein 1 homolog; UFD1; UFD1_HUMAN; UFD1L;

Immunogens

Immunogen:
Uniprot:

Q92890(UFD1_HUMAN) >>Visit HPA database.

Gene(ID):
UFD1(7353)
Expression:
Q92890 UFD1_HUMAN:

Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.

Sequence:
MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERQVQHEESTEGEADHSGYAGELGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKKVEEDEAGGRFVAFSGEGQSLRKKGRKP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Chicken
100
Rabbit
92
Xenopus
75
Bovine
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92890 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S3 Phosphorylation
S27 Phosphorylation
S29 Phosphorylation
K45 Ubiquitination
K68 Ubiquitination
K72 Ubiquitination
T78 Phosphorylation
K124 Ubiquitination
S129 Phosphorylation
T136 Phosphorylation
K139 Ubiquitination
K165 Ubiquitination
R170 Methylation
K175 Ubiquitination
K178 Ubiquitination
K198 Ubiquitination
S209 Phosphorylation
T210 Phosphorylation
S217 Phosphorylation
Y219 Phosphorylation
S229 Phosphorylation
S231 Phosphorylation
K238 Acetylation
K240 Ubiquitination
S245 Phosphorylation
S247 Phosphorylation
K250 Acetylation
K250 Ubiquitination
K255 Ubiquitination
Y261 Phosphorylation
K264 Ubiquitination
K267 Ubiquitination
K279 Ubiquitination
K280 Ubiquitination
S294 Phosphorylation
S299 Phosphorylation
K302 Ubiquitination

Research Backgrounds

Function:

Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I.

Subcellular Location:

Nucleus. Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.

Subunit Structure:

Heterodimer with NPLOC4, this heterodimer binds VCP and inhibits Golgi membrane fusion. Interacts with USP13. Interacts with ZFAND2B; probably through VCP.

Family&Domains:

Belongs to the UFD1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

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