TSHR Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from Hela, using TSHR Antibody.

DF12492 at 1/100 staining Mouse testis tissue by IHC-P.

Product:	TSHR Antibody
Catalog:	DF12492
Description:	Rabbit polyclonal antibody to TSHR
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	62-70 kDa; 87kD(Calculated).
Uniprot:	P16473
RRID:	AB_2845297

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TSHR Antibody detects endogenous levels of total TSHR.

RRID:

AB_2845297
Cite Format: Affinity Biosciences Cat# DF12492, RRID:AB_2845297.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CHNG1; hTSHR I; hTSHRI; LGR 3; LGR3; MGC75129; Seven transmembrane helix receptor; Thyroid adenoma hyperfunctioning; Thyroid carcinoma with thyrotoxicosis; Thyroid stimulating hormone receptor; Thyroid stimulating hormone receptor, isoform 2; Thyroid-stimulating hormone receptor; Thyrotropin receptor; Thyrotropin receptor I; Thyrotropin receptor I, hTSHR I; TSH R; TSH-R; TSHR; TSHR_HUMAN;

Immunogens

Immunogen:

Uniprot:

P16473(TSHR_HUMAN) >>Visit HPA database.

Gene(ID):

TSHR(7253)

Expression:

P16473 TSHR_HUMAN:

Expressed in thyroide cells (at protein level) (PubMed:11847099). Expressed in the thyroid (PubMed:2610690).

Sequence:

P16473 TSHR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRPADLLQLVLLLDLPRDLGGMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSMQSLRQRKSVNALNSPLHQEYEENLGDSIVGYKEKSKFQDTHNNAHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTICGDSEDMVCTPKSDEFNPCEDIMGYKFLRIVVWFVSLLALLGNVFVLLILLTSHYKLNVPRFLMCNLAFADFCMGMYLLLIASVDLYTHSEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWYAITFAMRLDRKIRLRHACAIMVGGWVCCFLLALLPLVGISSYAKVSICLPMDTETPLALAYIVFVLTLNIVAFVIVCCCYVKIYITVRNPQYNPGDKDTKIAKRMAVLIFTDFICMAPISFYALSAILNKPLITVSNSKILLVLFYPLNSCANPFLYAIFTKAFQRDVFILLSKFGICKRQAQAYRGQRVPPKNSTDIQVQKVTHDMRQGLHNMEDVYELIENSHLTPKKQGQISEEYMQTVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16473 As Substrate

P16473

Site	PTM Type	Source
K42	Ubiquitination	Uniprot
K58	Ubiquitination	Uniprot
N77	N-Glycosylation	Uniprot
N99	N-Glycosylation	Uniprot
N113	N-Glycosylation	Uniprot
N177	N-Glycosylation	Uniprot
N198	N-Glycosylation	Uniprot
S268	Phosphorylation	Uniprot
S298	Phosphorylation	Uniprot
N302	N-Glycosylation	Uniprot
S308	Phosphorylation	Uniprot
S425	Phosphorylation	Uniprot
Y706	Phosphorylation	Uniprot
Y739	Phosphorylation	Uniprot
S745	Phosphorylation	Uniprot
T748	Phosphorylation	Uniprot

Research Backgrounds

P16473_TSHR_HUMAN

Function:

Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism (By similarity).

PTMs:

Glycosylated.

Sulfated. Sulfation on Tyr-385 plays a role in thyrotropin receptor binding and activation.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Basolateral cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed in thyroide cells (at protein level). Expressed in the thyroid.

Subunit Structure:

Interacts with heterodimer GPHA2:GPHB5; this interaction stimulates cAMP production. Interacts (via the PDZ-binding motif) with SCRIB; regulates TSHR trafficking and function.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Immune diseases > Autoimmune thyroid disease.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

References

1). Thyrotropin induces atherosclerosis by upregulating large conductance Ca2+-activated K+ channel subunits. Molecular and cellular endocrinology, 2024 (PubMed: 38184154) [IF=4.1]

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TSHR Antibody - #DF12492