Syntaxin 17 Antibody - #DF12483
Product: | Syntaxin 17 Antibody |
Catalog: | DF12483 |
Description: | Rabbit polyclonal antibody to Syntaxin 17 |
Application: | WB IHC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
Mol.Wt.: | 33 kDa; 33kD(Calculated). |
Uniprot: | P56962 |
RRID: | AB_2845288 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12483, RRID:AB_2845288.
Fold/Unfold
FLJ20651; MGC102796; MGC126613; MGC126615; Stx17; STX17_HUMAN; Syntaxin 17; Syntaxin-17;
Immunogens
- P56962 STX17_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEDEEKVKLRRLEPAIQKFIKIVIPTDLERLRKHQINIEKYQRCRIWDKLHEEHINAGRTVQQLRSNIREIEKLCLKVRKDDLVLLKRMIDPVKEEASAATAEFLQLHLESVEELKKQFNDEETLLQPPLTRSMTVGGAFHTTEAEASSQSLTQIYALPEIPQDQNAAESWETLEADLIELSQLVTDFSLLVNSQQEKIDSIADHVNSAAVNVEEGTKNLGKAAKYKLAALPVAGALIGGMVGGPIGLLAGFKVAGIAAALGGGVLGFTGGKLIQRKKQKMMEKLTSSCPDLPSQTDKKCS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P56962 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Acetylation | Uniprot | |
S2 | Phosphorylation | Uniprot | |
K19 | Ubiquitination | Uniprot | |
K34 | Ubiquitination | Uniprot | |
K41 | Acetylation | Uniprot | |
K41 | Ubiquitination | Uniprot | |
K88 | Ubiquitination | Uniprot | |
S99 | Phosphorylation | Uniprot | |
T102 | Phosphorylation | Uniprot | |
K117 | Ubiquitination | Uniprot | |
Y157 | Phosphorylation | P00519 (ABL1) | Uniprot |
S202 | Phosphorylation | Uniprot | |
K219 | Ubiquitination | Uniprot | |
Y227 | Phosphorylation | Uniprot | |
K273 | Ubiquitination | Uniprot | |
T287 | Phosphorylation | Uniprot | |
S289 | Phosphorylation | Uniprot | |
T297 | Phosphorylation | Uniprot |
Research Backgrounds
SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.
Phosphorylated at Tyr-157 probably by ABL1. Dephosphorylation by PTPN2; regulates exit from the endoplasmic reticulum (By similarity).
Endoplasmic reticulum membrane>Multi-pass membrane protein. Smooth endoplasmic reticulum membrane>Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane>Multi-pass membrane protein. Cytoplasmic vesicle>Autophagosome membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein. Cytoplasm>Cytosol.
Note: Has a hairpin-like insertion into membranes. Localizes to the completed autophagosome membrane upon cell starvation (PubMed:23217709). May also localize to the mitochondria according to PubMed:23217709.
Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome. Interacts with VAMP7 and VTI1B. Probably interacts with BET1, SCFD1 and SEC22B (By similarity). Interacts with PTPN2 and ABL1; involved in STX17 phosphorylation. Interacts with COPB1 (By similarity). Interacts with TMED9 and TMED10; the interaction is direct. Interacts with ATG14. Interacts with RUBCNL/PACER; promoting targeting of RUBCNL/PACER to autophagosome.
Belongs to the syntaxin family.
Research Fields
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.
References
Application: WB Species: Mice Sample: CD4 + T cells
Application: WB Species: mouse Sample: CD4+T cells
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