Syntaxin 17 Antibody | Affinity Biosciences

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Syntaxin 17 Antibody - Western blot analysis of extracts from mouse testis tissue, using Syntaxin 17 Antibody.

Product:	Syntaxin 17 Antibody
Catalog:	DF12483
Description:	Rabbit polyclonal antibody to Syntaxin 17
Application:	WB IHC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	33 kDa; 33kD(Calculated).
Uniprot:	P56962
RRID:	AB_2845288

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(82%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Syntaxin 17 Antibody detects endogenous levels of total Syntaxin 17.

RRID:

AB_2845288
Cite Format: Affinity Biosciences Cat# DF12483, RRID:AB_2845288.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ20651; MGC102796; MGC126613; MGC126615; Stx17; STX17_HUMAN; Syntaxin 17; Syntaxin-17;

Immunogens

Immunogen:

A synthesized peptide derived from human Syntaxin 17, corresponding to a region within C-terminal amino acids.

Uniprot:

P56962(STX17_HUMAN) >>Visit HPA database.

Gene(ID):

STX17(55014)

Sequence:

P56962 STX17_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSEDEEKVKLRRLEPAIQKFIKIVIPTDLERLRKHQINIEKYQRCRIWDKLHEEHINAGRTVQQLRSNIREIEKLCLKVRKDDLVLLKRMIDPVKEEASAATAEFLQLHLESVEELKKQFNDEETLLQPPLTRSMTVGGAFHTTEAEASSQSLTQIYALPEIPQDQNAAESWETLEADLIELSQLVTDFSLLVNSQQEKIDSIADHVNSAAVNVEEGTKNLGKAAKYKLAALPVAGALIGGMVGGPIGLLAGFKVAGIAAALGGGVLGFTGGKLIQRKKQKMMEKLTSSCPDLPSQTDKKCS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Rabbit

100

Horse

Sheep

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P56962_STX17_HUMAN

Function:

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.

PTMs:

Phosphorylated at Tyr-157 probably by ABL1. Dephosphorylation by PTPN2; regulates exit from the endoplasmic reticulum (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Smooth endoplasmic reticulum membrane>Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane>Multi-pass membrane protein. Cytoplasmic vesicle>Autophagosome membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein. Cytoplasm>Cytosol.
Note: Has a hairpin-like insertion into membranes. Localizes to the completed autophagosome membrane upon cell starvation (PubMed:23217709). May also localize to the mitochondria according to PubMed:23217709.

Family&Domains:

Belongs to the syntaxin family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

References

1). mTOR deletion ameliorates CD4+ T cell apoptosis during sepsis by improving autophagosome-lysosome fusion. APOPTOSIS, 2022 (PubMed: 35435531) [IF=6.1]

Application: WB Species: Mice Sample: CD4 + T cells

Fig. 3 Transcription factor EB (TFEB) phosphorylation and SNARE expression in septic mice. (A, a–e) Protein levels of phospho (p)-TFEB, syntaxin 17 (STX17), vesicle associated membrane protein 8 (VAMP8), and synaptosomal-associated protein 29 (SNAP29) in CD4 + T cells were quantified by western blotting in wild-type (WT) sham-treated mice, WT CLP mice, mammalian target of rapamycin (mTOR) knockout via Cre expression under the control of lymphocyte-specific protein tyrosine kinase (Lck) (Lck-mTOR) CLP mice, and tuberous sclerosis complex 1 (TSC1) knockout (Lck-TSC1) CLP mice. The amount of each protein level was normalized to that of β-actin. Means ± standard deviations (SDs) of four mice per group are shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Application: WB Species: mouse Sample: CD4+T cells

Fig. 3| Transcription factor EB (TFEB) phosphorylation and SNARE expression in septic mice. (A, a–e) Protein levels of phospho (p)-TFEB, syntaxin 17 (STX17), vesicle associated membrane protein 8 (VAMP8), and synaptosomal-associated protein 29 (SNAP29) in CD4+T cells were quantifed by western blotting in wild-type (WT)sham-treated mice, WT CLP mice, mammalian target of rapamycin(mTOR) knockout via Cre expression under the control of lymphocyte-specifc protein tyrosine kinase (Lck) (Lck-mTOR) CLP mice,and tuberous sclerosis complex 1 (TSC1) knockout (Lck-TSC1) CLP mice.

2). Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis. Nutrients, 2022 (PubMed: 35277017) [IF=5.9]

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Syntaxin 17 Antibody - #DF12483