SUGT1 Antibody - #DF12481

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Product: SUGT1 Antibody
Catalog: DF12481
Description: Rabbit polyclonal antibody to SUGT1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Chicken
Mol.Wt.: 38 kDa and 41 kDa; 41kD(Calculated).
Uniprot: Q9Y2Z0
RRID: AB_2845286

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Sheep(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
SUGT1 Antibody detects endogenous levels of total SUGT1.
RRID:
AB_2845286
Cite Format: Affinity Biosciences Cat# DF12481, RRID:AB_2845286.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Protein 40 6 3; Protein 40-6-3; Protein SGT1 homolog; Putative 40 6 3 protein; Sgt1; SGT1 suppressor of G2 allele of SKP1; SGT1_HUMAN; SGT1B protein; sugt1; Suppressor of G2 allele of SKP1 homolog; Suppressor of G2 allele of SKP1 S. cerevisiae homolog of;

Immunogens

Immunogen:
Uniprot:

Q9Y2Z0(SGT1_HUMAN) >>Visit HPA database.

Gene(ID):
SUGT1(10910)
Sequence:
MAAAAAGTATSQRFFQSFSDALIDEDPQAALEELTKALEQKPDDAQYYCQRAYCHILLGNYCVAVADAKKSLELNPNNSTAMLRKGICEYHEKNYAAALETFTEGQKLDIETGFHRVGQAGLQLLTSSDPPALDSQSAGITGADANFSVWIKRCQEAQNGSESEVWTHQSKIKYDWYQTESQVVITLMIKNVQKNDVNVEFSEKELSALVKLPSGEDYNLKLELLHPIIPEQSTFKVLSTKIEIKLKKPEAVRWEKLEGQGDVPTPKQFVADVKNLYPSSSPYTRNWDKLVGEIKEEEKNEKLEGDAALNRLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKKY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Chicken
92
Zebrafish
83
Xenopus
75
Horse
50
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2Z0 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T8 Phosphorylation
S11 Phosphorylation
S17 Phosphorylation
S19 Phosphorylation
K41 Ubiquitination
Y47 Phosphorylation
Y48 Phosphorylation
C49 S-Nitrosylation
K70 Ubiquitination
K85 Ubiquitination
C88 S-Nitrosylation
K93 Ubiquitination
Y95 Phosphorylation
K171 Ubiquitination
K194 Ubiquitination
K204 Ubiquitination
K211 Ubiquitination
K221 Ubiquitination
K256 Ubiquitination
T265 Phosphorylation
K267 Ubiquitination
K274 Ubiquitination
Y277 Phosphorylation
S279 Phosphorylation
S281 Phosphorylation
Y283 Phosphorylation
T284 Phosphorylation
K289 Ubiquitination
K295 Sumoylation
K299 Ubiquitination
K302 Ubiquitination
Y317 Phosphorylation
S318 Phosphorylation
S321 Phosphorylation
K325 Ubiquitination
K330 Ubiquitination
S331 Phosphorylation P53350 (PLK1)
S335 Phosphorylation
T338 Phosphorylation
S345 Phosphorylation
K349 Ubiquitination
K351 Ubiquitination
Y365 Phosphorylation

Research Backgrounds

Function:

May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.

PTMs:

Phosphorylated at Ser-281 and Ser-331, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocates to the nucleus upon heat shock, requiring S100A6.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Probably associates with SCF (SKP1-CUL1-F-box protein) complex through interaction with SKP1. Interacts with S100A6. Interacts with HSP90.

Family&Domains:

The CS domain mediates interaction with HSP90.

Belongs to the SGT1 family.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

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