SRP54 Antibody - #DF12479

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Product: SRP54 Antibody
Catalog: DF12479
Description: Rabbit polyclonal antibody to SRP54
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 54 kDa; 56kD(Calculated).
Uniprot: P61011
RRID: AB_2845284

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
SRP54 Antibody detects endogenous levels of total SRP54.
RRID:
AB_2845284
Cite Format: Affinity Biosciences Cat# DF12479, RRID:AB_2845284.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Signal recognition particle 54 kDa protein; Signal recognition particle 54kDa; SRP 54; SRP54; SRP54_HUMAN;

Immunogens

Immunogen:
Uniprot:

P61011(SRP54_HUMAN) >>Visit HPA database.

Gene(ID):
SRP54(6729)
Sequence:
MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61011 As Substrate

Site PTM Type Enzyme
R8 Methylation
T11 Phosphorylation
S12 Phosphorylation
S18 Phosphorylation
T21 Phosphorylation
K33 Acetylation
K50 Acetylation
K50 Ubiquitination
S58 Phosphorylation
K71 Ubiquitination
K73 Ubiquitination
K81 Acetylation
K81 Ubiquitination
K85 Ubiquitination
K92 Ubiquitination
K98 Ubiquitination
K149 Ubiquitination
S192 Phosphorylation
K306 Ubiquitination
K311 Ubiquitination
K321 Ubiquitination
K323 Ubiquitination
K398 Ubiquitination
K426 Ubiquitination
K438 Ubiquitination
K442 Ubiquitination
K448 Ubiquitination
S451 Phosphorylation
S453 Phosphorylation
K457 Ubiquitination
K464 Ubiquitination
K496 Methylation
K496 Ubiquitination

Research Backgrounds

Function:

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

Subcellular Location:

Nucleus speckle. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. Interacts with RNPS1.

Family&Domains:

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.

Belongs to the GTP-binding SRP family. SRP54 subfamily.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein export.

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