Product: SHMT1 Antibody
Catalog: DF12474
Source: Rabbit
Application: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 53 kD; 53kD(Calculated).
Uniprot: P34896
RRID: AB_2845279

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SHMT1 Antibody detects endogenous levels of total SHMT1.
RRID:
AB_2845279
Cite Format: Affinity Biosciences Cat# DF12474, RRID:AB_2845279.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CSHMT; Cytoplasmic serine hydroxymethyltransferase; cytosolic; GLYC_HUMAN; Glycine hydroxymethyltransferase; Serine hydroxymethyltransferase 1 (soluble); Serine hydroxymethyltransferase; Serine hydroxymethyltransferase, cytosolic; Serine methylase; SHMT; Shmt1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MTMPVNGAHKDADLWSSHDKMLAQPLKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADENGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVKSVDPKTGKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPDF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
92
Chicken
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P34896 As Substrate

Site PTM Type Enzyme
K10 Acetylation
K10 Ubiquitination
K20 Ubiquitination
K27 Ubiquitination
S29 Phosphorylation
Y34 Phosphorylation
K38 Sumoylation
K38 Ubiquitination
K39 Sumoylation
K72 Ubiquitination
Y73 Phosphorylation
K98 Ubiquitination
Y118 Phosphorylation
K157 Acetylation
K157 Ubiquitination
K158 Acetylation
K159 Ubiquitination
S161 Phosphorylation
S169 Phosphorylation
K173 Ubiquitination
Y180 Phosphorylation
Y183 Phosphorylation
K196 Ubiquitination
K271 Acetylation
K274 Acetylation
K279 Acetylation
K375 Acetylation
K375 Ubiquitination
K386 Ubiquitination
T405 Phosphorylation
K416 Ubiquitination
K420 Ubiquitination
K446 Ubiquitination
K449 Ubiquitination
K456 Ubiquitination

Research Backgrounds

Function:

Interconversion of serine and glycine.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer. Identified in complex with ABRAXAS2 and the other subunits of the BRISC complex, at least composed of ABRAXAS2, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1.

Family&Domains:

Belongs to the SHMT family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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