Product: Seladin 1 Antibody
Catalog: DF12472
Description: Rabbit polyclonal antibody to Seladin 1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 60 kDa; 60kD(Calculated).
Uniprot: Q15392
RRID: AB_2845277

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Seladin 1 Antibody detects endogenous levels of total Seladin 1.
RRID:
AB_2845277
Cite Format: Affinity Biosciences Cat# DF12472, RRID:AB_2845277.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

24 dehydrocholesterol reductase; 24-dehydrocholesterol reductase; 3 beta hydroxysterol delta 24 reductase; 3-beta-hydroxysterol delta-24-reductase; DCE; Delta(24)-sterol reductase; Desmosterol to cholesterol enzyme; DHC24_HUMAN; Dhcr24; Diminuto dwarf1 homolog; Diminuto/dwarf1 homolog; KIAA0018; Nbla03646; Seladin-1; SELADIN1; Selective AD indicator 1; Selective Alzheimer's Disease Indicator 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q15392 DHC24_HUMAN:

Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus, hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression in the inferior temporal lobe is substantially lower than in the frontal cortex.

Sequence:
MEPAVSLAVCALLFLLWVRLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKTHKNIMINLMDILEVDTKKQIVRVEPLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRCTPSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQRQENHFVEGLLYSLDEAVIMTGVMTDEAEPSKLNSIGNYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFILPSQPGLVHPKGNEAELYIDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAARH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Zebrafish
80
Chicken
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15392 As Substrate

Site PTM Type Enzyme
K76 Ubiquitination
K82 Ubiquitination
K87 Ubiquitination
T110 Phosphorylation
Y180 Phosphorylation
T188 Phosphorylation
Y190 Phosphorylation
S198 Phosphorylation
K254 Ubiquitination
Y299 Phosphorylation
Y300 Phosphorylation
K301 Ubiquitination
K313 Methylation
K313 Ubiquitination
Y321 Phosphorylation
K367 Ubiquitination
T373 Phosphorylation
K376 Ubiquitination
K427 Ubiquitination
K446 Sumoylation
K446 Ubiquitination
K459 Ubiquitination
K492 Ubiquitination
K496 Ubiquitination
Y507 Phosphorylation
K509 Ubiquitination
K512 Ubiquitination

Research Backgrounds

Function:

Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. In addition to its cholesterol-synthesizing activity, can protects cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Golgi apparatus membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus, hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression in the inferior temporal lobe is substantially lower than in the frontal cortex.

Subunit Structure:

Interacts with DHCR7; this interaction regulates DHCR7 activity.

Family&Domains:

Belongs to the FAD-binding oxidoreductase/transferase type 4 family.

Research Fields

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Effects of Fdft 1 gene silencing and VD3 intervention on lung injury in hypoxia-stressed rats. Genes & Genomics, 2022 (PubMed: 35947298) [IF=2.1]

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