SAT1 Antibody - #DF12469

Fig. 4: MNT regulates ferroptosis by binding to the SAT1 promoter region and repressing its transcription. a Venn plot showing the intersection of predicted targets of MNT, the 33 key ferroptosis genes were intersected with the results of ChIP-seq, obtaining 12 gene sets that may be target genes regulated by MNT. b qRT-PCR results showed differences in RNA levels of the key molecules (GPX4, SLC7A11, ASCL4 and SAT1) of ferroptosis between the MNT-NC, MNT-OE and MNT-sh of A549 and PC9 cell lines. c Western blot results showed differences in protein levels of GPX4, SLC7A11, ASCL4, and SAT1 of ferroptosis between the MNT-NC, MNT-OE, and MNT-sh of A549 and PC9 cell lines. d Genome-wide data from the ENCODE project and ChIP-seq results show the solid MNT-binding peak in the promoter region close to the TSS of SAT1. e Characteristic sequences predicted by JASPAR for transcriptional binding of MNT. f, g ChIP-qPCR assay depicting the enrichments of MNT binding in A549 and PC9. h Schematic diagram showing the possible binding sites of MNT to the critical region of the SAT1 promoter are mutated. i Dual luciferase activity assays to analyze the fluorescence intensity of MNT-NC and MNT-OE with or without mutations in the SAT1 promoter region. Data were analyzed by Student’s t-test and presented by mean ± SD in triplicate. ns, not significant