RAB14 Antibody - #DF12460

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Product: RAB14 Antibody
Catalog: DF12460
Description: Rabbit polyclonal antibody to RAB14
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 24 kDa; 24kD(Calculated).
Uniprot: P61106
RRID: AB_2845265

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RAB14 Antibody detects endogenous levels of total RAB14.
RRID:
AB_2845265
Cite Format: Affinity Biosciences Cat# DF12460, RRID:AB_2845265.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bA165P4.3; F protein binding protein 1; FBP; GTPase Rab14; RAB 14; RAB14; RAB14 member RAS oncogene family; RAB14_HUMAN; Ras related protein Rab 14; Ras-related protein Rab-14; RP11 165P4.4; Small GTP binding protein RAB14;

Immunogens

Immunogen:
Uniprot:

P61106(RAB14_HUMAN) >>Visit HPA database.

Gene(ID):
RAB14(51552)
Sequence:
MATAPYNYSYIFKYIIIGDMGVGKSCLLHQFTEKKFMADCPHTIGVEFGTRIIEVSGQKIKLQIWDTAGQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGENVEDAFLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQREGCGC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
64
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61106 As Substrate

Site PTM Type Enzyme
A2 Acetylation
Y8 Phosphorylation
Y14 Phosphorylation
C26 S-Nitrosylation
K34 Ubiquitination
K35 Ubiquitination
S56 Phosphorylation
K59 Ubiquitination
K61 Ubiquitination
T67 Phosphorylation
Y80 Phosphorylation
Y81 Phosphorylation
Y91 Phosphorylation
S97 Phosphorylation
Y99 Phosphorylation
S103 Phosphorylation
T107 Phosphorylation
K125 Ubiquitination
K140 Ubiquitination
K156 Ubiquitination
K170 Ubiquitination
K171 Ubiquitination
S180 Phosphorylation
K193 Ubiquitination

Research Backgrounds

Function:

Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes (By similarity). Regulates, together with its guanine nucleotide exchange factor DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion.

Subcellular Location:

Recycling endosome. Early endosome membrane>Lipid-anchor>Cytoplasmic side. Golgi apparatus membrane>Lipid-anchor>Cytoplasmic side. Golgi apparatus>trans-Golgi network membrane>Lipid-anchor>Cytoplasmic side. Cytoplasmic vesicle>Phagosome.
Note: Recruited to recycling endosomes by DENND6A (PubMed:22595670). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with KIF16B (By similarity). Interacts with ZFYVE20.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

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